RESUMO
Laparoscopes can suffer from fogging and contamination difficulties, resulting in a reduced field of view during surgery. A series of diamond-like carbon films, doped with SiO, were produced by pulsed laser deposition for evaluation as biocompatible, antifogging coatings. DLC films doped with SiO demonstrated hydrophilic properties with water contact angles under 40°. Samples subjected to plasma cleaning had improved contact angle results, with values under 5°. Doping the DLC films with SiO led to an average 40% decrease in modulus and 60% decrease in hardness. Hardness of the doped films, 12.0 - 13.2 GPa, was greater than that of the uncoated fused silica substrate, 9.2 GPa. The biocompatibility was assessed through CellTiter-Glo assays, with the films demonstrating statistically similar levels of cell viability when compared to the control media. The absence of ATP released by blood platelets in contact with the DLC coatings suggests in vivo hemocompatibility. The SiO doped films displayed improved transparency levels in comparison to undoped films, achieving up to an average of 80% transmission over the visible spectrum and an attenuation coefficient of 1.1 × 104 cm-1 at the 450 nm wavelength. The SiO doped DLC films show promise as a method of fog prevention for laparoscopes.
Assuntos
Anestésicos/efeitos adversos , Halotano/efeitos adversos , Hipertermia Maligna/induzido quimicamente , Acidose/tratamento farmacológico , Animais , Anuros , Bicarbonatos/farmacologia , Cafeína/farmacologia , Cálcio/metabolismo , Temperatura Baixa , Modelos Animais de Doenças , Eletrólitos/sangue , Enzimas/sangue , Halotano/farmacologia , Manitol/farmacologia , Músculos/efeitos dos fármacos , Procaína/farmacologia , Ratos , Retículo Sarcoplasmático/metabolismo , Succinilcolina/farmacologia , Suínos , Trometamina/farmacologiaRESUMO
Multiple exposures to halothane have been shown to have delayed effects on bromsulphthalein (B.S.P.) clearance. Rats were exposed to repeated halothane anaesthetics, and their livers were subsequently isolated and perfused. B.S.P. retention in the perfusate of these isolated livers was greatly increased one to three weeks after the last halothane exposure. In similarly pretreated animals at the same time period the B.S.P.-glutathione conjugating enzyme activity in homogenates of the livers was found to be depressed. These findings did not occur after multiple diethyl ether exposures or following a single exposure to halothane.