RESUMO
Different methods of laboratory diagnostics were comparatively analyzed in examining 25 patients at the early infection stage. Sera were measured by using various serological reactions. Specific antibodies were determined by using the reaction of indirect immune-fluorescence (RIIF), the immune-enzyme analysis (IEA) and the complement-binding reaction (CBR) in 83.1%, 54.4% and 12.5% of cases, respectively. Essential differences in sensitivity were detected between the above methods. RIIF was proven to be a reliable and sufficiently sensitive method in the laboratory diagnostics of Lyma's disease. While the use of two methods, i.e. RIIF and IEA, ensures the highest percentage of detection of antibodies to the causative agent of Lyma's disease.
Assuntos
Doença de Lyme/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antibacterianos/sangue , Grupo Borrelia Burgdorferi/imunologia , Testes de Fixação de Complemento , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Técnicas Imunoenzimáticas , Doença de Lyme/sangue , Doença de Lyme/microbiologia , Masculino , Pessoa de Meia-Idade , Sensibilidade e EspecificidadeRESUMO
During examination of 60 children aged 3-15 years with manifestations of acute bronchitis Chlamydia pneumoniae antigen was detected in their nasal and pharynx smears in 11.7% of cases with the use of enzyme immunoassay and indirect immunofluorescence test. In 10% of cases chlamydial pneumonia was diagnosed serologically. In the course of complex laboratory diagnostics C. pneumoniae was detected in 13.3% of cases. The most complete information could be obtained by a combination of different methods of the antigen detection as well as specific IgG and IgM antibodies.
Assuntos
Bronquite/microbiologia , Chlamydophila pneumoniae , Doença Aguda , Adolescente , Bronquite/epidemiologia , Criança , Pré-Escolar , Chlamydophila pneumoniae/imunologia , Humanos , Imunoglobulina A/sangue , Imunoglobulina G/sangueRESUMO
175 children with acute bronchopulmonary pathology were examined for the presence of the pneumotropic infective agents by serological and bacteriological methods. In most children microbial associations with the prevalence of mycoplasmas, pneumocycts and, to a lesser extent, chlamydiae were detected. The considerable activation of cytomegalovirus (CMV) infection in children with pneumonia and bronchitis was noted. Taking into account the results of the examination and the clinico-anamnestic data, the character of infections could be established: chlamydial and CMV infections were mainly persisting; pneumococcal infection was mainly acute, hemophilic and pneumocyst infections were mixed. The clinical picture of acute pneumonia cases had characteristic features determined by the supposed etiological agent, but this picture could change under the influence of pneumotropic infective agents.