RESUMO
Factor IX antigen and activity were assayed in a group of patients with liver disease and in a group of patients in coumarin therapy. In patients with liver disease there was a similar decrease in activity and antigen. On the other hand factor IX activity is decreased in coumarin treatment with factor IX antigen remaining normal. Factor IX electrophoretic mobility in liver disease is normal.
Assuntos
Transtornos da Coagulação Sanguínea/diagnóstico , Fator IX/análise , Hepatopatias/diagnóstico , Cumarínicos/uso terapêutico , Hemofilia B/diagnóstico , Humanos , Imunoensaio , Vitamina KAssuntos
Deficiência do Fator XIII/genética , Adulto , Testes de Coagulação Sanguínea , Fator XIII/análise , Deficiência do Fator XIII/complicações , Deficiência do Fator XIII/diagnóstico , Feminino , Imunofluorescência , Hemorragia/etiologia , Transtornos Hemorrágicos/etiologia , Humanos , Imunoeletroforese , Imunoeletroforese Bidimensional , Linhagem , Hemorragia Pós-Parto/etiologia , GravidezAssuntos
Antígenos/análise , Fator IX/imunologia , Hemofilia B/imunologia , Epitopos , Humanos , MétodosAssuntos
Transtornos da Coagulação Sanguínea/genética , Fator VII/genética , Adolescente , Adulto , Antígenos/análise , Transtornos da Coagulação Sanguínea/sangue , Reações Cruzadas , Fator VII/imunologia , Feminino , Heterozigoto , Humanos , Linhagem , Tempo de Protrombina , Tromboplastina/metabolismoRESUMO
Rabbit raised anti-alpha-1-antitrypsin or anti alpha-2-macroglobulin antisera at dilution of less than 1:80 yielded non-specific staining on human platelets by indirect immunofluorescent technique. A similar pattern was in fact obtained by using normal rabbit sera at the same dilution and was due to the presence of smooth muscle autoantibodies. This indicates that human platelets do not contain significant quantities of these antigens. In agreement with the above, only microamounts of alpha-1-antitrypsin and alpha-2-macroglobulin were found to be present in human platelets by means of the electroimmunoassay.
Assuntos
Plaquetas/análise , alfa 1-Antitripsina/sangue , alfa-Macroglobulinas/sangue , Imunofluorescência , HumanosRESUMO
An excess of factor IX antigen or protein with respect to factor IX activity is present in coumarin treated patients. The average factor IX antigen value found in a group of 16 patients was 96.2 (S.D. = 24.46) whereas the average clotting activity was 19 (S.D. = 4.54) (p less than 0.001). In the electroimmunoassay system a normal peak or precipitate were seen in every instance. In haemophilia B--no peak or precipitate were seen. The coumarin induced abnormal factor IX shows a more anodic migration in the bidimensional immunoelectrophoresis system as compared with the normal counterpart. On the contrary, the factor IX protein present in haemophilia BM or in haemophilie B+ migrates as normal factor IX.
Assuntos
Antígenos/análise , Cumarínicos/efeitos adversos , Fator IX/imunologia , Fator IX/fisiologia , Humanos , Soros ImunesRESUMO
Immunological and immunofluorescent studies carried out on plasma and platelets of three cases of congenital factor XIII deficiency are reported. Two of these patients were originally thought to have normal factor XIII subunit S and no subunit A. However, repeated assays carried out using different lots of antiserum showed that in reality the patients lacked both subunit S and subunit A. The false positive finding was due to the presence of a anti-factor VIII contaminant in the antiserum originally used. The third patient had a normal subunit S and no subunit A. No factor XIII antigen was found by the indirect immunofluorescent technique in normal, factor XIII deficiency and von Willebrand's disease platelets. On the contrary, by using the non-monospecific antiserum a fluorescent pattern similar to that observed by using an anti-factor VIII antiserum, had been noted. On the basis of the data presented in this paper a tentative classification of factor XIII deficiency in two groups is proposed: Type I is characterized by the lack of both factor XIII subunit S and A. Type II is characterized by a normal subunit S and no subunit A. The need for a re-evaluation of published case of factor XIII deficiency by means of monospecific antisera is indicated.
Assuntos
Antígenos/análise , Deficiência do Fator XIII/imunologia , Fator XIII/imunologia , Plaquetas/imunologia , Deficiência do Fator XIII/classificação , Feminino , Humanos , Soros Imunes , Doenças de von Willebrand/imunologiaRESUMO
Two sisters born from a nonconsanguineous marriage were found to have congenital factor XIII deficiency. In the electroimmunoassay system, using an anti-subunit S antiserum, two distinct peaks or rockets were seen in normal plasma and serum whereas only one peak was present in the propositae plasma or serum. In the bidimensional immunoelectrophoresis system, using the anti-subunit S antiserum, two major peaks were seen in normal plasma whereas only one peak was seen in the propositae plasma. Using an anti-subunit A antiserum no peak or precipitate was seen in our propositae in the electroimmunoassay or in the bidimensional immunoelectrophoresis systems. Both the parents and the children of our two propositae showed a normal coagulation pattern. Therefore, the heredity appears to be autosomal recessive. These data indicate that the defect is characterized by a normal factor XIII subunit S (support) and a lack of factor XIII subunit A (activity).
Assuntos
Deficiência do Fator XIII/congênito , Adulto , Testes de Coagulação Sanguínea , Deficiência do Fator XIII/genética , Feminino , Humanos , Imunoensaio , ImunoeletroforeseRESUMO
23 patients with hemophilia B have been investigated by means of several immunological methods. 16 patients (69.9%) had no detectable factor XI antigen. Five had a normal factor IX antigen and the electrophoretic mobility of this abnormal factor IX was similar to that of its normal counterpart. One of these five patients had hemophilia Bm, since ox brain thromboplastin clotting time was severely prolonged. The remaining two patients had reduced or decreased factor IX antigen. Several patients showed a slight protongation of ox brain thromboplastin time due to an associated slight factor VII deficiency. On the basis of these results, a tentative classification of hemophilia B into five variants is proposed, namely: hemctor IX antigen; hemophilia Bra, or with reduced factor IX antigen; hemophilia Bm, or with normal factor IX antigen and severely prolonged ox brain thromboplastin; hemophilia B patients is feasible only by means of a battery of tests, namely:factor IX activity assay, factor IX antigen determination, ox brain thromboplastin clotting time, factor VII activity assay.
Assuntos
Hemofilia B/imunologia , Fator IX/análise , Fator VII/análise , Fator XI/análise , Variação Genética , Hemofilia B/classificação , Humanos , Isoantígenos/análiseRESUMO
Activation of coumarin plasmas in glass tubes for 60 min resulted in a clear shortening of Thrombotest clotting times. Normotest clotting times were shortened too but to a much lesser extent. As a consequence the Normotest (NT)/Thrombotest (TT) discrepancy which was quite large at 0 time, became progressively smaller. This phenomenon was observed both in undiluted and 6:10 diluted plasma. After ellagic acid activation a similar phenomenon was noted even though a less pronounced shortening of Thrombotest was noted. These data suggest that Thrombotest is very sensitive to contact phase and to factor VII activation and not to any coumarin-induced inhibitors.
Assuntos
Testes de Coagulação Sanguínea , Cumarínicos , Ácido Elágico/farmacologia , Fator VII , Vidro , Humanos , Fatores de TempoRESUMO
Tranexamic acid in a dose of 50 mg/kg b.w. was unable to alter the ellagic acid induced hypercoagulable state. No change in the hypercoagulability pattern was observed regardless of the time of administration of the compound (before or after the ellagic infusion). The silicone clotting times after the infusion of ellagic acid were markedly shortened and remained so for about 60 minutes. The results observed in two control groups treated with saline were similar. The euglobulin lysis times were clearly prolonged after the administration of tranexamic acid, whereas no changes were observed after the administration of saline. These results indicate that tranexamic acid has no anti-factor XII activity.
Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Ácidos Cicloexanocarboxílicos/farmacologia , Ácido Tranexâmico/farmacologia , Animais , Cães , Ácido Elágico , Fator XII/antagonistas & inibidores , Feminino , Fibrinólise , Humanos , MasculinoRESUMO
A patient with a combined hereditary deficiency of factors VII and VIII is presented together with a family study. The main bleeding manifestations were easy bruising and bleeding after tooth extractions. No hemarthrosis was ever observed. The main laboratory features consisted in a mild prolongation of prothrombin time and of partial thromboplastin time. TG test was abnormal and was corrected by the addition of adsorbed normal plasma. Specific assays revealed a moderate defect of factors VII and VIII. All other clotting factors were within normal limits. The factor VII antigen in the propositus was normal or nearly normal. The factor-VIII-associated antigen was also normal. Five additional family members presented the same coagulation pattern and were variably symptomatic. The hereditary transmission pattern seems to be autosomal dominant. The defect appears to be due to a structural abnormality of a gene controlling factors VII and VIII activation.