Designing a new scoring system (QualyP Score) correlating the management of cardiopulmonary bypass to postoperative outcomes.

AIM: The aim of this study was to ascertain if a score, directly derived from CPB records, could correlate to major postoperative outcomes. METHODS: An additive score (QualyP Score) was created from 10 parameters: peak lactate value during CPB, peak VCO(2)i, lowest DO(2)i/VCO(2)i, peak respiratory quotient, CPB time, cross-clamp time, lowest CPB temperature, circulatory arrest, ultrafiltration during CPB, number of packed red cells transfused intraoperatively. The PerfSCORE was calculated, as well. Multivariable logistic regression models were built to detect the independent predictors of: peak lactate >3 mmol/L during the first three postoperative days; the incidence of acute kidney injury network (AKIN) 1-2-3; respiratory insufficiency; mortality. RESULTS: The mean score was 4.8±2.6 (0-10). A QualyP Score ≥1 was predictive of postoperative acidosis (OR=1.595). A score ≥2 was predictive of AKIN 2 (OR=1.268) and respiratory insufficiency (OR=1.526). A score ≥5 was predictive of AKIN 3 (OR=1.848) and mortality (OR=1.497). CONCLUSIONS: QualyP Score may help to provide a quality marker of perfusion, emphasizing the need for goal-directed perfusion strategies.

The RNA-binding protein Sam68 contributes to proliferation and survival of human prostate cancer cells.

The tyrosine kinase Src is frequently activated in advanced human prostate carcinomas and its activation correlates with tyrosine phosphorylation of the RNA-binding protein Sam68. Herein, we have investigated the expression and function of Sam68 in human prostate cancer cells. Analysis of specimens obtained from 20 patients revealed that Sam68 is upregulated at the protein level in 35% of the samples. Real-time polymerase chain reaction confirmed the results at the mRNA level in most patients. Downregulation of Sam68 by RNAi in LNCaP prostate cancer cells delayed cell cycle progression and reduced the proliferation rate. Moreover, depletion of Sam68 sensitized cells to apoptosis induced by DNA-damaging agents. Similarly, stable cell lines expressing a truncated GFP-Sam68(GSG) protein displayed reduced growth rates and higher sensitivity to cisplatin-induced apoptosis. Microarray analyses revealed that a subset of genes involved in proliferation and apoptosis were altered when Sam68 was knocked down in LNCaP cells. Our results indicate that Sam68 expression supports prostate cancer cells proliferation and survival to cytotoxic agents.

Neovascularized bone grafts: experimental investigation.

Vascularized bone grafts are standardized procedures in reconstructive surgery but there are some disadvantages: donor site morbidity, limited number of "natural" donor sites, and complex technique. In this study, we test the possibility of creating a "neovascularized" bone graft utilizing a vascular implantation procedure in a rabbit model. Sixteen New Zealand adult white rabbits were used. In each animal, two iliac crest bone grafts (7 x 7 x 10 mm) were harvested. Vascular implantation of the right superficial femoral vessels was performed in one of the two grafts, which was wrapped in a silicone envelope to avoid neovascularization from the surrounding tissues and positioned in a subcutaneous pocket in the right medial thigh. On the left side, the bone block, wrapped in the silicone envelope, was buried subcutaneously without vascular implantation. The operated animals were divided into two groups: Group I included eight rabbits explanted 4 weeks postoperatively and Group II included eight rabbits explanted 8 weeks postoperatively. Tetracycline injection was performed 72 hours preexplantation to evaluate new bone formation. Selective colloidal ink injection in the axial artery was performed to investigate the neovascularization before inclusion in poly-methyl-methacrylate (PMMA). Histological examination was performed in all explanted specimens comparatively. Histological examination 8 weeks after surgery showed a marked neovascularization, with normal bone cells. Tetracycline labeling showed new bone formation with a normal pattern. In all nonvascularized specimens, no viable cells or neovascularization and no bone formation were found. The vascular implantation procedure can induce a good neovascularization with new bone formation in a small bone graft. The possibility of neovascularization induction by the simple vascular implantation procedure has several clinical implications in reconstructive surgery.

The heterodigital reversed flow neurovascular island flap for fingertip injuries.

We report the results of pulp reconstruction with a new heterodigital reverse flow island flap. A dorsolateral flap from the middle phalanx, based on the digital artery is raised from the adjacent uninjured finger. The common digital artery, between the injured finger and the donor finger, is ligated and transected just before its bifurcation. The two converging branches of the digital arteries can be entirely mobilized as a continuous vascular pedicle for the flap. Thus the vascularization is now supplied by reverse flow through the proximal transverse digital palmar arch of the injured finger. To provide sensation the dorsal branch of the proper digital nerve from the donor finger can be included in the flap. Six reverse heterodigital island flaps were used in patients. In five patients the flap was used for pulp reconstruction and in one case for covering a dorsal digital defect. In one case mild venous congestion occurred. Good skin coverage with supple and well-vascularized skin was obtained in each patient. The static two-point discrimination over the flap was between 6 and 15 mm. This new procedure is indicated for extensive pulp defects in fingers in which reconstruction cannot be done using other flaps and as an alternative to microsurgical reconstruction.

Divergent dislocation of the fourth and fifth carpometacarpal joints.

A case of divergent dislocation of the fourth and fifth carpometacarpal joints is reported. The injury resulted from a high-energy trauma and was treated by closed reduction and cast immobilization for 4 weeks. Evaluation at the 26-month follow-up visit showed good radiographic and functional results.

Replantation of degloved skin of the hand.

The treatment of a degloving injury is one of the most difficult problems in hand surgery. Various reconstructive procedures have been adopted in the past years, all with poor results. Between 1988 and 1995, nine patients with degloving injuries of the hand and fingers were treated by microsurgical replantation. The injury involved the thumb in three patients, the ring finger in three patients, the little finger in one patient, and multiple fingers in two patients. Successful complete revascularization was obtained in seven patients. In one case a superficial necrosis of the replanted thumb skin occurred with good preservation of the subcutaneous layer. In one patient with a degloving injury involving multiple fingers, revascularization was achieved only in the middle finger, and the first ray was secondarily resurfaced by a free flap from the foot. In our experience revascularization of the degloved skin does represent the best solution and must be managed as an emergency procedure. Coverage obtained in this way offers the best cosmetic result and allows early mobilization with good recovery of joint movement. Reestablishing sensibility is more difficult. It is not always possible to suture the nerves damaged by the trauma, and even when a careful primary nerve anastomosis is performed, the results often are unsatisfactory, probably because of the avulsive mechanism of nerve injury.

Complex traumatic lesions of the hand. Definition and surgical treatment.

Complex traumatic lesions of the hand constitute a frequent problem in surgical reconstruction. Often young subjects of full work age and with high functional requirements are affected. Thus, microsurgery has changed the quoad functionem prognosis of these lesions. In addition to presenting a modern definition of complex lesions, the authors expose and discuss a rational method of surgical treatment in light of the most recent possibilities of reconstruction offered by microsurgery of flaps and reimplantations. The conclusion is that only the constant presence of an expert microsurgical team permits a correct approach to this type of lesion.

Resorbable poly-L-lactic acid mini-staples for the fixation of Akin osteotomies.

The authors present a new fixation system constituted by reabsorbable mini-staples in poly-L-lactic acid, the use of which is proposed for Akin osteotomies. An initial cohort of 25 cases (23 patients), with a mean follow-up of 16 months (minimum 6 months, maximum 30 months) is presented. In 24 cases union of the osteotomy was found within 30 days of the surgical treatment; however, in one case it took longer (12 weeks). Neither pseudoarthrosis, malunion, nor breakage of the mini-staples was found. No adverse tissue reactions were observed. In all cases good deformity correction was achieved, with patient satisfaction reported in about 96% of the cases. In this type of surgery and in selected cases (without osteoporosis and/or severe arthritis of the first metatarsophalangeal joint), this fixation device is an interesting alternative to the traditional nonabsorbable types.

Microsurgical approach for a venous malformation of the thumb.

We report a case of a low-flow venous malformation of the thumb treated by microsurgical excision of the mass and a free toe pulp flap. Forty-two months after surgery the patient had a good cosmetic and functional results without any recurrence of the tumour.

Homodigital neurovascular island flaps with "direct flow" vascularization.

Pulp loss of the fingers is frequently observed. When local advancement flaps are not sufficient to repair the defect, the homodigital monopedicled island flaps with "direct flow" are a simple and current reconstructive solution. Between 1991 and 1995, 32 homodigital neurovascular direct flow island flaps were performed for fingertip defects. We used a modified triangular Venkataswami flap for oblique amputations (26 patients) and the Mouchet-Gilbert island flap for tangential defects (6 patients). Our results (with excellent skin quality and good sensory recovery) lead us to consider these flaps as the first-choice reconstructive solution in patients with extensive but not complete pulp defects (< 2 cm) of the fingers.

Austin/chevron osteotomy fixed with bioabsorbable poly-L-lactic acid single screw.

The authors propose a new bioabsorbable fixation device to be applied to V-shaped transverse osteotomies of the head of the first metatarsal (Austin-chevron osteotomies) for hallux valgus surgery. Namely, a 3.3-mm. diameter cortical screw made of poly-L-lactic acid. This screw was applied in 30 patients with an average age of 44 years (range: 20 to 61). A total of 35 osteotomies was performed. The study refers to the period between 1992 and 1994 with a mean follow-up of 18 months (range: 12 to 36 months). The results of the study demonstrate good stability of the synthesis, with normal union (mean: 4 weeks), no adverse tissue reactions or osteolytic phenomena were noted. One patient developed avascular necrosis of the metatarsal head, which in the opinion of the authors, was not due to the device implanted. Over 90% of the patients were satisfied with both the aesthetic and functional results. The authors conclude that the poly-L-lactic acid cortical screw is a promising bioabsorbable fixation device for this corrective osteotomy in selected patients less than 50 years old, with good first metatarsal bone stock.

Osteosarcoma of the scaphoid. A case report and review of the literature.

We describe a case of osteosarcoma of the scaphoid bone, which to our knowledge is only the second reported case of osteosarcoma in the carpus. A 38-year-old man complained of intense pain in the right wrist and had curettage and a bone graft for a lesion in the scaphoid. Histological examination showed this to be an osteosarcoma. Below-elbow amputation was performed and adjuvant chemotherapy given. There has been no evidence of recurrence or metastases at 33 months after amputation.

Ring avulsion injuries: microsurgical management.

Microsurgical treatment of ring avulsion injuries is often difficult, due to widespread intimal damage of the digital vascular bundles. Thirty-one patients with such injuries in varying degrees were treated during the period 1986 to 1992: of these, 15 underwent microsurgical revascularization. In addition to the traditional procedures applied in seven patients (arteriolysis, direct vessel suture, vein grafts), a technique consisting of vessel transfer from the middle finger was also employed. In five patients, the ulnar digital artery of the middle finger was transferred to the ring finger. In four patients, at least one vein was transferred from the dorsal aspect of the middle finger. Twelve of 15 microreconstructions were successful: with an average follow-up of 48.3 months, these patients showed very good functional (mean total active range of motion: 234 degrees and mean s2PD: 9.8mm) and cosmetic recovery. These results lead to the conclusion that, except for cases characterized by proximal amputation at the flexor superficialis tendon insertion, microsurgical treatment should always be carried out.

Acute posterior interosseous nerve palsy caused by a synovial haemangioma of the elbow joint.

A case is described of spontaneous posterior interosseous nerve palsy of acute onset. The initial symptoms made it difficult to distinguish the condition from tendon rupture. The diagnosis was established using ultrasound, nerve conduction studies and MRI. The patient underwent surgery to decompress the posterior interosseous nerve and the histological examination identified the tumour as a synovial haemangioma. 12 months after the operation, the patient had made a complete recovery, confirmed by EMG.

Reverse neurovascular homodigital island flap.

We describe a homodigital island flap with a reverse vascular pedicle based on the anastomoses between the radial and ulnar digital arteries. These anastomotic branches lie between the posterior wall of the tendon sheath and the periosteum to form the three digitopalmar arches. The vascularization of the reverse homodigital island flap is obtained using the middle transverse palmar arch. This flap was performed in 11 patients as a neurovascular "sensitive" flap, including the digital nerve in the pedicle, which was then sutured to the contralateral one at the defect. This technique achieves cover of the tactile pad in one operative stage and provides well-vascularized skin, allowing early mobilization. The quality of skin cover was confirmed in all the patients at follow-up ranging from 7 to 43 months postoperatively. We believe that the benefits of the procedure outweigh the disadvantages related to the section of the digital nerve and artery.

Transfer of vessels in the management of thumb and ring avulsion injuries.

The main problem in avulsion or degloving injury is the extensive damage to long segments of vessels which makes direct suture of the structures difficult. Various techniques have been proposed over the years to bypass the segment of an injured vessel. Many of these are difficult and often require the execution of several vascular anastomoses. The transfer of vessels from adjacent fingers, as proposed by Doi, is a valid alternative. During the 5-year period from 1988 to 1993, vessel transfer from adjacent fingers was carried out on 15 patients. Three patients suffered incomplete amputation and 5 patients suffered complete amputation of the thumb. In 5 cases these were severe crush injuries and in 3 cases they were avulsion injuries. Seven patients suffered ring avulsion injuries of various degrees. The simplicity and reliability of the method (one case was unsuccessful) make this an important technique in the treatment of crush and avulsion injuries of the thumb as well as of ring avulsion injuries.

Kiloh-Nevin syndrome: a clinical case of compression of the anterior interosseous nerve.

Anterior interosseous nerve syndrome is a rare occurrence. One particular case, in terms of its etiopathogenesis, and its clinical findings that involved isolated lesion of the long flexor muscle of the thumb is reported. Healing occurred after approximately 10 months. Medical and physiotherapeutic treatment were carried out.

[A reverse vascular autograft finger island flap. A review of 15 cases and of the literature]. / A propos du lambeau en îlot homodigital à contre-courant. Révision de 15 cas et de la littérature.

The authors discuss the indications for homodigital island flap with a reverse vascular pedicle. This flap is based on the anastomoses between the radial and ulnar digital arteries. These anastomotic branches lie between the posterior wall of the tendon sheath and the periosteum to form an arch and are named the "digitopalmar arches". The vascularization of the reverse homodigital island flap is derived by using the middle transverse palmar arch. This flap was performed successfully in 14 patients involving 15 fingers to resurface amputation of the distal phalanx. In 6 cases the flap was used as an "artery" flap, and in 9 cases as a "sensitive" homodigital island flap. The pedicle in these cases was neurovascular also containing the digital nerve. The sensitivity of the flap was obtained by neurorraphy between the transposed digital nerve of the flap and the receiving digital nerve of the recipient finger. This technique achieves cover of the tactile pad in one operative stage and provides well vascularized skin allowing early mobilization. Sensation of the flap can be restored rapidly when neurorraphy of the transposed digital nerve is performed.

The "kite flap" for dorsal thumb reconstruction.

Skin cover for losses of substance on the dorsum of the thumb is often difficult. The "kite flap", introduced by Foucher, is ideal for this purpose. In the period between 1988 and 1993, this flap was used in five patients presenting with skin defects on the dorsum of the thumb. The results obtained have convinced us to consider this technique as the first method of choice in this area of reconstructive surgery.