RESUMO
To detect the presence of variability in the tandemly repeated sequences of the Epstein-Barr virus latent origin of replication, we analyzed the length of the family of repeats in 14 lymphoblastoid and Burkitt's lymphoma cell lines by PCR amplification. The gel electrophoresis analysis of the PCR products revealed a broad banding pattern, characteristic of each line, consisting of several fragments, sometimes smeared, of variable length. This finding was interpreted as a result of the hairpin-like structures generated by the palindrome within the family of repeats, able to originate artefacts. Since the banding pattern was different only in strictly non-correlated cell lines, we supposed that the sequence of the repeat units was polymorphic. We therefore sequenced the family of repeats in three healthy bone marrow derived lymphoblastoid cell lines carrying an endogenous EBV as well as in a B95-8 infected cell line as control. The sequence analysis revealed that each line is different both in the number and in the sequence of repeats. At the 3' end of the family of repeats the B95-8 virus was found to have a 252 bp region missing in the GenBank standard sequence. This one is probably a partial sequence since it was shorter than the control specimens obtained from different sources of B95-8 DNA analyzed by Southern blot hybridization. The length analysis of the family of repeats can be used to characterize EBV strains by PCR.
Assuntos
DNA Viral/genética , Herpesvirus Humano 4/genética , Sequências Repetitivas de Ácido Nucleico/genética , Origem de Replicação , Sequência de Bases , Southern Blotting , Linhagem Celular , Heterogeneidade Genética , Humanos , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Alinhamento de Sequência , Homologia de Sequência do Ácido NucleicoRESUMO
The occurrence, upon differentiation, of a transient DNA hypomethylation has been observed in Friend erythroleukemia cells. Treatment with hexamethylenebisacetamide (HMBA) induces within 24 h a 20% hypomethylation of newly synthesized DNA, that is followed by re-methylation before completion of the differentiative process, as measured by the appearance of benzidine-positive cells. We examined a series of mutant clones which continue to grow in the presence of an inducer. Methylcytosine content of DNA was measured by HPLC, after cell labeling with [3H]uridine. We found that one of these continuously growing clones, which was still capable of hemoglobin synthesis, showed the same degree of hypomethylation as the parental one. The re-methylation process did not occur, however, unless erythroid differentiation was reverted by the removal of the inducer. In another clone which had lost the capacity to synthesize hemoglobin, no DNA hypomethylation was detectable. These experiments show that DNA hypomethylation is an early event strictly related to cell differentiation but not to cell growth arrest.
Assuntos
DNA/metabolismo , Vírus da Leucemia Murina de Friend , Leucemia Eritroblástica Aguda/genética , Acetamidas/farmacologia , Animais , Diferenciação Celular/efeitos dos fármacos , Células Clonais , Citosina/análogos & derivados , Citosina/análise , DNA/biossíntese , Hemoglobinas/biossíntese , Leucemia Eritroblástica Aguda/metabolismo , Metilação/efeitos dos fármacos , Camundongos , TrítioRESUMO
The extents of thialysine and selenalysine incorporation into cell proteins were compared in E. coli KL16 and in a mutant able to grow equally well in the presence or in the absence of both lysine analogs. The mutant differs from the parental strain in the repression of aspartokinase III (AKIII), the first enzyme of the lysine biosynthetic pathway. No analog incorporation into proteins was observed in mutant cells grown in the presence of either analog, whereas a marked analog incorporation was observed in the parental strain, where up to 17% and 12% of protein lysine can be substituted by thialysine and selenalysine respectively. In the parental strain grown in media containing either analog at different concentration the extent of analog incorporation into proteins is related to the extent of AKIII repression.
Assuntos
Aspartato Quinase/biossíntese , Escherichia coli/metabolismo , Lisina/análogos & derivados , Lisina/metabolismo , Compostos Organosselênicos , Biossíntese de Proteínas , Aspartato Quinase/genética , Cisteína/análogos & derivados , Cisteína/metabolismo , Repressão Enzimática , Escherichia coli/genética , Isoenzimas , Mutação , Selênio/metabolismoRESUMO
The extent of protein lysine substitution by thialysine in E. coli cells grown in media containing the analog depends on the time interval the cells are grown in the presence of analog and on the analog concentration in the medium. By calculating the percent of lysine substitution in newly synthesized proteins it was shown that this reaches, after one cell doubling in the presence of analog, a maximum which is 17% in the cells grown with 0.1 or 0.2 mM thialysine and 8% in cells grown with 0.05 mM thialysine. Proteins synthesized in the presence of analog in the concentration range 0.05-0.2 mM show similar stability to those synthesized in the absence of analog. The extent of analog incorporation into newly synthesized proteins, as regards both the time course and the dependence on analog concentration in the medium, is strictly related to the extent of the repression of AK III, the first enzyme of lysine biosynthetic pathway.
Assuntos
Aspartato Quinase/metabolismo , Proteínas de Bactérias/biossíntese , Cisteína/análogos & derivados , Escherichia coli/metabolismo , Fosfotransferases/metabolismo , Proteínas de Bactérias/análise , Cisteína/metabolismo , Escherichia coli/crescimento & desenvolvimento , Lisina/análise , Fatores de TempoAssuntos
Escherichia coli/efeitos dos fármacos , Compostos Organosselênicos , Ovário/citologia , Inibidores da Síntese de Proteínas/farmacologia , Selênio/farmacologia , Animais , Células Cultivadas , Cricetinae , Cricetulus , Cisteína/farmacologia , Resistência a Medicamentos , Escherichia coli/genética , Escherichia coli/fisiologia , Feminino , Lisina/metabolismo , Lisina/farmacologia , Lisina-tRNA Ligase/antagonistas & inibidores , Lisina-tRNA Ligase/metabolismo , Mutação , Ovário/efeitos dos fármacosRESUMO
A thialysine-resistant mutant of the E. coli KL16 strain was isolated. It can grow equally well in the presence and in the absence of thialysine. The properties of the two lysine transport systems, of the lysyl-tRNA synthetase and of the aspartokinase III (AK III) were studied in the mutant and in the parent strain. AK III is the first enzyme of the lysine biosynthetic pathway and its activity is involved in the regulation of lysine biosynthesis by feed-back and repression mechanism. No difference between the two strains was evidenced as regards 1) the affinity of the transport systems for lysine and thialysine 2) the activity of the lysyl-tRNA synthetase 3) the allosteric inhibition of the AK III by lysine and thialysine. A marked difference between the two strains has been evidenced in the AK III repression: in the mutant the enzyme is much less repressed both by lysine and thialysine. The possible correlation between the activity of AK III and the thialysine-resistance is discussed in this paper.
Assuntos
Aspartato Quinase/metabolismo , Cisteína/análogos & derivados , Escherichia coli/enzimologia , Fosfotransferases/metabolismo , Aspartato Quinase/antagonistas & inibidores , Aspartato Quinase/genética , Transporte Biológico Ativo , Cisteína/farmacologia , Resistência Microbiana a Medicamentos/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Cinética , Lisina/metabolismo , Lisina/farmacologia , Lisina-tRNA Ligase/metabolismo , MutaçãoRESUMO
Thialysine and selenalysine can be utilized for protein synthesis by lysine-requiring E. coli cells even in the absence of lysine. Protein synthesis has been determined as labeled leucine incorporation into acid-insoluble material, as increase of cell proteins and as protein-lysine substitution by the analog. Either analog can be incorporated into proteins, in the absence of lysine, for a limited time interval after which cells stop to duplicate. Proteins synthesized during this period contain most of their lysine residues substituted by the analog. Moreover, it has been shown that the analog-containing proteins are unstable and rapidly degraded. Their instability would account for the inability of lysine-requiring E. coli cells to utilize the analog as growth factor.
Assuntos
Proteínas de Bactérias/metabolismo , Cisteína/análogos & derivados , Escherichia coli/metabolismo , Lisina/análogos & derivados , Compostos Organosselênicos , Selênio/metabolismo , Proteínas de Bactérias/biossíntese , Cisteína/metabolismo , Escherichia coli/crescimento & desenvolvimento , Leucina/biossíntese , Lisina/metabolismoRESUMO
In E. coli, Se-3 aminopropylselenocysteine or selenahomolysine (SeHL) does not affect intracellular lysine transport, i.e. it cannot bind E. coli lysine transport systems. In CHO cells it inhibits cationic aminoacid transport system, but only in the presence of Na+, this indicating that it behaves like polar neutral aminoacids. On the other hand, it poorly affects leucine transport both in the presence and in the absence of Na+. SeHL is not activated by aminoacyl-tRNA synthetase preparations from bacterial and mammalian sources, thus it cannot be utilized for protein synthesis.
Assuntos
Aminoácidos/metabolismo , Aminoacil-tRNA Sintetases/metabolismo , Lisina/análogos & derivados , Compostos Organosselênicos , Selênio/metabolismo , Animais , Arginina/metabolismo , Transporte Biológico , Células Cultivadas , Cricetinae , Cricetulus , Escherichia coli/metabolismo , Lisina/metabolismo , Sódio/farmacologiaAssuntos
Redutases do Citocromo/metabolismo , Leucemia Eritroblástica Aguda/enzimologia , Animais , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular , Citocromo-B(5) Redutase , Dimetil Sulfóxido/farmacologia , Eritrócitos/enzimologia , Vírus da Leucemia Murina de Friend , Histocitoquímica , Leucemia Eritroblástica Aguda/patologia , Masculino , Camundongos , Camundongos Endogâmicos DBARESUMO
Thialysine and selenalysine cannot substitute lysine as a growth factor for a lysine-requiring E. coli mutant, but can nevertheless be utilized for protein synthesis in the presence of lysine. In order to have information about the effects of lysine on the utilization of the two analogs, the extent of the incorporation of the three aminoacids into newly synthesized proteins has been determined. The analog starts to be utilized by cells growing in a medium containing either analog and lysine when lysine concentration becomes very low. Of the two analogs, thialysine is more easily utilized. In fact thialysine can be utilized when the lysine/thialysine ratio in the medium is 1/25. Selenalysine starts to be utilized when the lysine/selenalysine ratio is 1/200.
Assuntos
Cisteína/análogos & derivados , Escherichia coli/crescimento & desenvolvimento , Lisina/análogos & derivados , Lisina/metabolismo , Compostos Organosselênicos , Selênio/metabolismo , Cisteína/metabolismo , Escherichia coli/genética , Isoleucina/metabolismo , Cinética , Leucina/metabolismo , MutaçãoRESUMO
A thialysine-resistant mutant of E. coli strain KL16 also shows a lower sensitivity to selenalysine, the lysine analog containing selenium. No difference between the mutant and the parental strain has been shown regarding the affinities of the transport systems and the lysyl-tRNA synthetase for selenalysine, thialysine and lysine as well as the inhibitory effects of these three aminoacids on the activity of the lysine biosynthetic pathway. A marked difference between the two strains has been evidenced in the AK III repression: in the mutant the repression by selenalysine, thialysine and lysine is much lower than in the parental strain.
Assuntos
Cisteína/análogos & derivados , Escherichia coli/genética , Lisina/análogos & derivados , Compostos Organosselênicos , Selênio/farmacologia , Aspartato Quinase/biossíntese , Cisteína/farmacologia , Resistência Microbiana a Medicamentos , Repressão Enzimática , Escherichia coli/efeitos dos fármacos , Escherichia coli/enzimologia , Cinética , Lisina/farmacologia , Lisina-tRNA Ligase/metabolismo , MutaçãoRESUMO
The intracellular transport and the activation of lysine, thialysine and selenalysine have been investigated in a thialysine-resistant CHO cell mutant strain in comparison with the parental strain. The cationic amino acid transport system responsible for the transport of these 3 amino acids shows no differences between the 2 strains as regards its affinity for each of these amino acids. On the other hand the Vmax of the transport system in the mutant is about double that in the parental strain. The lysyl-tRNA synthetase, assayed both as ATP = PPi exchange reaction and lysyl-tRNA synthesis, shows a lower affinity for thialysine and selenalysine than for lysine in both strains; in the mutant, however, the difference is even greater. Thus the thialysine resistance of the mutant is mainly due to the properties of its lysyl-tRNA synthetase, which shows a greater difference of the affinities for lysine and thialysine with respect to the parental strain.
Assuntos
Cisteína/análogos & derivados , Compostos Organosselênicos , Trifosfato de Adenosina/metabolismo , Animais , Arginina/metabolismo , Transporte Biológico , Cricetinae , Cisteína/metabolismo , Cisteína/toxicidade , Difosfatos/metabolismo , Resistência a Medicamentos , Cinética , Lisina/análogos & derivados , Lisina/farmacologia , Lisina-tRNA Ligase/antagonistas & inibidores , Selênio/farmacologiaRESUMO
CHO cells can incorporate thialysine and selenalysine in their proteins in substitution of lysine. Data are reported in the present paper showing that proteins containing either thialysine or selenalysine are unstable and quite rapidly degraded. The degradation rate is strictly related to the extent of protein lysine substitution. At similar extent of substitution, selenalysine-containing proteins are more unstable that thialysine-containing ones.
Assuntos
Cisteína/análogos & derivados , Lisina/análogos & derivados , Compostos Organosselênicos , Proteínas/metabolismo , Selênio/metabolismo , Aminoácidos/análise , Animais , Células Cultivadas , Cricetinae , Cricetulus , Cisteína/metabolismo , Isoleucina/metabolismo , Leucina/metabolismo , Lisina/metabolismo , Biossíntese de Proteínas , Hidrolisados de Proteína/análiseRESUMO
In E. coli aminoethylhomocysteine (AEHC) and aminopropylcysteine (APC) do not affect intracellular lysine transport thus showing that they cannot bind the E. coli lysine transport systems. In CHO cells AEHC and APC inhibit lysine and arginine transport, AEHC more than APC, thus indicating that they can bind the cationic aminoacid transport system. They inhibit also leucine transport, APC more than AEHC. Some possible relationships between their structure and their effects on transport systems are considered. AEHC and APC are not activated by aminoacyl-tRNA synthetase preparations from bacterial and mammalian sources.
Assuntos
Aminoácidos/metabolismo , Aminoacil-tRNA Sintetases/metabolismo , Cisteína/análogos & derivados , Homocisteína/análogos & derivados , Animais , Arginina/metabolismo , Sítios de Ligação , Transporte Biológico , Cricetinae , Cricetulus , Cisteína/fisiologia , Escherichia coli/metabolismo , Homocisteína/fisiologia , Lisina/metabolismo , Relação Estrutura-AtividadeRESUMO
CHO cells can incorporate into proteins both thialysine and selenalysine when both are present together in the culture medium. Thialysine and selenalysine inhibit cell growth and cell viability. The inhibitory effect of either analog is additive. The inhibition of cell viability is related to the extent of protein lysine substitution by thialysine or selenalysine; it is however irrelevant whether lysine is substituted by one or the other analog or by both.
Assuntos
Cisteína/análogos & derivados , Lisina/análogos & derivados , Compostos Organosselênicos , Biossíntese de Proteínas , Selênio/metabolismo , Animais , Divisão Celular , Linhagem Celular , Sobrevivência Celular , Cricetinae , Cisteína/metabolismo , Inibidores do Crescimento , Lisina/metabolismoRESUMO
The intracellular transport of thialysine and selenalysine in CHO cells has been studied. Data have been obtained indicating that the two lysine analogs can be transported by both the cationic aminoacid transport system and by the L transport system. The affinity of the cationic aminoacid transport system is similar for the two lysine analogs but lower than that for lysine and the affinity of the L transport system for the two lysine analogs is lower than that for leucine.
Assuntos
Aminoácidos/metabolismo , Cisteína/análogos & derivados , Lisina/análogos & derivados , Compostos Organosselênicos , Selênio/metabolismo , Aminoácidos/farmacologia , Animais , Transporte Biológico/efeitos dos fármacos , Linhagem Celular , Cricetinae , Cricetulus , Cisteína/metabolismo , Cisteína/farmacologia , Feminino , Cinética , Lisina/metabolismo , Lisina/farmacologia , Ovário , Selênio/farmacologiaRESUMO
CHO cells allowed to grow in a medium containing selenalysine can utilize it for protein synthesis. Selenalysine is incorporated into cell proteins in substitution of lysine: a maximum of 5% of protein lysine can be substituted. Protein lysine substitution by selenalysine can be correlated to the reduced viability of cells grown in its presence.
Assuntos
Lisina/análogos & derivados , Compostos Organosselênicos , Selênio/metabolismo , Animais , Linhagem Celular , Cricetinae , Cricetulus , Feminino , Cinética , Lisina/metabolismo , Lisina/farmacologia , OvárioRESUMO
A thialisyne resistant variant clone of CHO cells also shows a lower sensitivity to selenasyne, the lysine analog containing selenium. Growth rate, cell viability and protein synthesis rate are less affected by selenasyne in the variant compared to the parental strain. Data are reported showing that during cellular growth of either strain some toxic derivatives of selenasyne are produced and accumulated in the culture medium even in the presence of excess lysine.
Assuntos
Divisão Celular/efeitos dos fármacos , Cisteína/análogos & derivados , Lisina/análogos & derivados , Compostos Organosselênicos , Selênio/farmacologia , Animais , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Células Clonais , Meios de Cultura , Cisteína/farmacologia , Resistência a Medicamentos , Lisina/farmacologia , Biossíntese de ProteínasRESUMO
Thialysine cannot completely substitute lysine as growth factor for a lysine-requiring E. coli mutant. However it can be utilized for growth in the presence of limiting amounts of lysine, in substitution of, and in competition with this latter. The effects of thialysine on growth rate, protein synthesis rate and cell viability, and its incorporation into proteins were studied in function of lysine and thialysine concentration in the culture media. Up to 60% of protein lysine substitution by thialysine is observed, without appreciable effects on cell viability.
Assuntos
Cisteína/análogos & derivados , Escherichia coli/metabolismo , Lisina/metabolismo , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/metabolismo , Cisteína/metabolismo , Cisteína/farmacologia , Escherichia coli/genética , Escherichia coli/crescimento & desenvolvimento , MutaçãoRESUMO
Data reported in this paper show that both lysine transport systems in E. coli KL16 can be repressed by lysine and its isologs, thialysine and selenalysine, whereas they are not repressed by ornithine. The repression is specific on lysine transport systems; it is evident with 0.01 mM lysine or isolog concentration and reaches a maximum with 0.1 mM concentration. By comparing the extent of repression by lysine and its isologs, lysine gives the highest and selenalysine the lowest degree of repression. The shift from the repressed to the depressed state is rather immediate once the amino acid is removed from the culture medium.