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1.
Drug Deliv Transl Res ; 9(1): 414, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30187351

RESUMO

In the original article text presenting and discussing results shown in Fig. 6 omitted to mention that quantification of TGF-ß2 and TGF-ß3 was not included in Fig. 6a, c, e.

2.
Drug Deliv Transl Res ; 8(5): 1014-1024, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29691813

RESUMO

Cytokines, chemokines, and growth and remodeling factors orchestrate wound healing when skin damage occurs. During early stages, when the wound is still open, detection and quantification of these compounds might provide biomarkers of skin wound healing, which could aid to complete the scenario provided by clinical follow-up data and histological and histomorphometric analyses. This work assessed and compared the healing of full-thickness skin wounds grafted with artificial dermis made with autologous skin fibroblasts and unidirectional or multidirectional type I collagen scaffolds to test this hypothesis. Biomarkers of healing were detected and quantified in the culture medium of artificial dermis and exudates from the grafted wounds. Clinical follow-up of animals and histological and histomorphometric analysis showed differences in graft integration, wound closure, and histological and histomorphometric parameters. Surface plasmon resonance quantification of 13 healing biomarkers indicated differential secretion of most of the quantified factors in culture medium by the multidirectional and unidirectional artificial dermis. Also, there were significant differences between the concentration of some of the factors analyzed in the exudates of wounds grafted with the evaluated artificial dermis. These findings suggest that differential delivery of healing biomarkers induced by the directionality of the scaffold used to produce the multidirectional and unidirectional dermis was sufficient to create two skin wound microenvironments that determined a different outcome of healing. Overall, data indicate that healing of wounds grafted with multidirectional autologous artificial dermis is better than that of the wounds grafted with the unidirectional one.


Assuntos
Biomarcadores/metabolismo , Epiderme/transplante , Dermatopatias/terapia , Cicatrização , Animais , Autoenxertos , Quimiocinas/metabolismo , Colágeno Tipo I/metabolismo , Citocinas/metabolismo , Modelos Animais de Doenças , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Dermatopatias/metabolismo , Transplante de Pele , Pele Artificial , Ressonância de Plasmônio de Superfície , Alicerces Teciduais
3.
Curr Protein Pept Sci ; 19(10): 958-971, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-28847291

RESUMO

Currently it is well known that all biological drugs, including those with a fully human structure, are capable of inducing a host immune response known as immunogenicity [1]. The presence of ADAs can condition the drug´s level and action, thus modifying the therapeutic effect and even the safety profile by its mechanism of action - neutralizing or non-neutralizing - and / or an increase in its clearance. Immunogenicity is a dynamic factor to be taken into account in biological therapy, especially in long-term treatments, and as a relevant aspect in the assessment of secondary response loss [2]. With the above, not only the knowledge but also the management of the immunogenicity of the different biological treatments, represent a useful instrument for optimization of the strategies of use for each drug, and in the design of predictive models of response, which finally permits a significant improvement in the efficacy and safety profile, aiming to a personalization of the therapies, especially in patients with autoimmune diseases, genetic disorders and cancer [3]. This review summarizes the events of immunogenicity that produce the biological drug, the factor that influence to immunogenicity and the assessment of immunogenicity.


Assuntos
Fatores Biológicos/imunologia , Proteínas/imunologia , Imunidade Adaptativa , Anticorpos/sangue , Anticorpos/imunologia , Doenças Autoimunes/tratamento farmacológico , Doenças Autoimunes/imunologia , Fatores Biológicos/farmacologia , Fatores Biológicos/uso terapêutico , Medicamentos Biossimilares/farmacologia , Medicamentos Biossimilares/uso terapêutico , Doenças Genéticas Inatas/tratamento farmacológico , Doenças Genéticas Inatas/imunologia , Humanos , Imunidade Ativa , Imunidade Humoral , Neoplasias/tratamento farmacológico , Neoplasias/imunologia , Peptídeos/imunologia , Peptídeos/farmacologia , Peptídeos/uso terapêutico , Proteínas/farmacologia , Proteínas/uso terapêutico
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