COVID-19 serological survey using micro blood sampling.

During August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Technology Graduate University (OIST), Japan, testing for the presence of antibodies against SARS-CoV-2, the causative agent of COVID-19. We used a FDA-authorized 2-step ELISA protocol in combination with at-home self-collection of blood samples using a custom low-cost finger prick-based capillary blood collection kit. Although our survey did not find any COVID-19 seropositive individuals among the OIST cohort, it reliably detected all positive control samples obtained from a local hospital and excluded all negatives controls. We found that high serum antibody titers can persist for more than 9 months post infection. Among our controls, we found strong cross-reactivity of antibodies in samples from a serum pool from two MERS patients in the anti-SARS-CoV-2-S ELISA. Here we show that a centralized ELISA in combination with patient-based capillary blood collection using as little as one drop of blood can reliably assess the seroprevalence among communities. Anonymous sample tracking and an integrated website created a stream-lined procedure. Major parts of the workflow were automated on a liquid handler, demonstrating scalability. We anticipate this concept to serve as a prototype for reliable serological testing among larger populations.

Ray Systems in Granular Cratering.

In classical experiments of granular cratering, a ball dropped on an evened-out bed of grains ends up within a crater surrounded by a uniform blanket of ejecta. In this Letter, we show that the uniform blanket of ejecta changes to a ray system, or set of radial streaks of ejecta, where the surface of the granular bed includes undulations, a factor that has not been addressed to date. By carrying out numerous experiments and computational simulations thereof, we ascertain that the number of rays in a ray system ∝D/λ, where D is the diameter of the ball and λ is the wavelength of the undulations. Further, we show that the ejecta in a ray system originates in a narrow annulus of diameter D with the center at the site of impact. Our findings may help shed light on the enigmatic ray systems that ring many impact craters on the Moon and other planetary bodies.

Detection of subcutaneous and intramuscular air with sonography: a sensitive and specific modality.

OBJECTIVES: Soft tissue air may raise suspicion for several life-threatening illnesses. Physical examination has limited sensitivity in detecting air, and computed tomography and magnetic resonance imaging are time-consuming and expensive. Sonography can show soft tissue air, but the sensitivity and specificity in this setting are currently unknown. Therefore, the purpose of this study was to assess the performance characteristics of sonography in depicting the presence, amount, and affected tissue plane in a cadaver model of soft tissue air. METHODS: We conducted a single-blinded observational cadaver study. Cutaneous sites were selected and marked (≈4 or 5 sites on each of 6 cadavers). An examiner injected air at some sites, recording both the depth (plane) and volume. A second examiner, who was blinded to the injection procedure, examined each site with sonography and recorded the presence or absence of air and the plane (subcutaneous or intramuscular). The results were compared, and statistical analysis was performed. RESULTS: Twenty-eight sites on 6 cadavers were used. Sites were not used if they were damaged or were within 10 cm of previous dissection. Air was injected in 20 of 28 sites; the remaining 8 were sham injections. Sonography showed air in all of the 20 sites with air injected. No air was detected in 7 of the 8 remaining sham sites, yielding 1 false-positive case. The plane was accurately assessed in all cases. Sensitivity for detection was 100%, and specificity was 87.5%. CONCLUSIONS: Sonography accurately showed the presence, amount, and anatomic plane of soft tissue air in cadavers with sensitivity of 100%. It warrants investigation as a screening test in patients at high risk for subcutaneous air from conditions such as necrotizing fasciitis and gas gangrene.