Addressing Water Contamination and Associated Health Issues through Community-Based Interventions: A Case Study in Khon Kaen Province.

A recent study conducted in Khon Kaen Province, Thailand, evaluated the effectiveness of a technology-assisted intervention aimed at improving water quality and addressing related health issues in communities around key water bodies. The intervention targeted health concerns associated with water contamination, including chronic kidney diseases, skin conditions, hypertension, and neurological symptoms. The study included water quality assessments and health evaluations of 586 residents and implemented a Learning Innovation Platform (LIP) across 13 communities. Results showed significant improvements in the community, including a decrease in hypertension and skin-related health issues, as well as enhanced community awareness and proficiency in implementing simple water quality assessments and treatment. The study demonstrated the value of a comprehensive, technology-driven community approach, effectively enhancing water quality and health outcomes, and promoting greater community awareness and self-sufficiency in managing environmental health risks.

Reduced graphene oxide on silver nanoparticle layers-decorated titanium dioxide nanotube arrays as SERS-based sensor for glyphosate direct detection in environmental water and soil.

When glyphosate, a widely used organophosphate herbicide in agricultural applications, contaminates the environment, it could lead to chronic harm to human health. Herein, an efficient, air-stable and reusable surface-enhanced Raman scattering (SERS) substrate was designed to be an analytical tool for direct determination of glyphosate. A vertical heterostructure of reduced graphene oxide (rGO)-wrapped dual-layers silver nanoparticles (AgNPs) on titania nanotube (TiO2 NTs) arrays was constructed as a SERS substrate. The TiO2 NTs/AgNPs-rGO exhibited high SERS performance for methylene blue detection, offering an analytical enhancement factor (AEF) as large as 7.1 × 108 and the limit of detection (LOD) as low as 10-14 M with repeatability of 4.4 % relative standard deviation (RSD) and reproducibility of 2.0 % RSD. The sensor was stable in ambient and was reusable after photo-degradation. The designed sensor was successfully applied for glyphosate detection with a LOD of 3 µg/L, which is below the maximum contaminant level of glyphosate in environmental water, as recommended by the U.S. EPA and the European Union. A uniqueness of this study is that there is no significant difference between the real-world applications of the SERS sensor on direct glyphosate analysis in environmental samples compared to an analysis using ultra-high performance liquid chromatography.

An electrochemical sensor for the voltammetric determination of artemisinin based on carbon materials and cobalt phthalocyanine.

An electrochemical sensor for the determination of artemisinin has been developed based on a glassy carbon electrode modified with hybrid nanocomposites of cobalt phthalocyanine, graphene nanoplatelets, multi-walled carbon nanotubes and ionic liquids (IL). To improve the sensitivity and selectivity of the sensor, cobalt phthalocyanine (CoPc) was used as an effective redox mediator to promote and catalyze the artemisinin reduction. Furthermore, the graphene nanoplatelets and multi-walled carbon nanotubes were used as excellent conducting supporting materials to improve the sensitivity of the electrochemical sensor. Moreover, IL with a surface charge was also employed to prevent aggregation of the graphene nanoplatelets and multi-walled carbon nanotubes. The analytical signal was generated from the reduction of Co(III)Pc generated by artemisinin. The proposed electrochemical sensor was applied to the detection of artemisinin using differential pulse voltammetry and provided a signal with wide linearity ranging from 1.5-60 µM and 60-600 µM and a detection limit of 0.70 µM (3SD/m). Furthermore, the proposed sensor displayed good repeatability and reproducibility of 2.9-3.0 and 3.1-4.4% RSD, respectively. Applications of the sensor to drug and plant samples demonstrated accuracy in a range of 105-116% recoveries. In addition, the results were in good agreement with those obtained from the HPLC method as a reference technique. Thus, the proposed electrochemical sensor provides a new alternative platform for sensitive and selective determination of artemisinin in the analysis of pharmaceuticals with good precision and accuracy.

Enzymatic electrochemical biosensor for glyphosate detection based on acid phosphatase inhibition.

A novel enzymatic electrochemical biosensor was fabricated for the indirect detection of glyphosate-based acid phosphatase inhibition. The biosensor was constructed on a screen-printed carbon electrode modified with silver nanoparticles, decorated with electrochemically reduced graphene oxide, and chemically immobilized with acid phosphatase via glutaraldehyde cross-linking. We measured the oxidation current by chronoamperometry. The current arose from the enzymatic reaction of acid phosphatase and the enzyme-substrate disodium phenyl phosphate. The biosensing response is a decrease in signal resulting from inhibition of acid phosphatase in the presence of glyphosate inhibitor. The inhibition of acid phosphatase by glyphosate was investigated as a reversible competitive-type reaction based on the Lineweaver-Burk equation. Computational docking confirmed that glyphosate was the inhibitor bound in the substrate-binding pocket of acid phosphatase and that it was able to inhibit the enzyme efficiently. Additionally, the established method was applied to the selective analysis of glyphosate in actual samples with satisfactory results following a standard method.

A portable selective electrochemical sensor amplified with Fe3O4@Au-cysteamine-thymine acetic acid as conductive mediator for determination of mercuric ion.

Mercury ion (Hg2+) is considered to be one of the most toxic heavy metal ions and can cause adverse effects on kidney function, the central nervous system, and the immune system. Therefore, it is important to develop a fast and simple method for sensitive and selective detection of Hg2+ in the environment. This research proposes a portable electrochemical sensor for rapid and selective detection of Hg2+. The sensor platform is designed based on thymine acetic acid anchored with cysteamine-conjugated core shell Fe3O4@Au nanoparticles (Fe3O4@Au/CA/T-COOH) immobilized on a sensing area of a screen-printed carbon electrode (SPCE) with the aid of an external magnetic field embedded in a homemade electrode holder for ease of handling. In the presence of Hg2+, the immobilized thymine combines specifically with Hg2+ and forms a thymine-Hg2+-thymine mismatch (T-Hg2+-T). The resulting amount of Hg2+ was determined by differential pulse anodic stripping voltammetry (DPASV). Under optimal conditions, the sensor exhibited two wide linearities in a range from 1 to 200 µg L-1 and 200-2200 µg L-1 with the reliability coefficient of determination of 0.997 and 0.999, respectively. The detection limit (LOD) and the quantification limit (LOQ) were also determined to be 0.5 µg L-1 and 1.0 µg L-1, respectively. The sensor was further applied for determination of Hg2+ in water samples, a certified reference material and fish samples. The results were compared with flow injection atomic spectroscopy-inductively coupled plasma-optical emission spectroscopy (FIAS-ICP-OES) systems as a reference method. Results obtained with the proposed sensor were relatively satisfactory, and they showed no significant differences at a 95% confidence level by t-test from the standard method. Therefore, considering its fast and simple advantages, this novel strategy provides a potential platform for construction of a Hg2+ electrochemical sensor.

An ultrasensitive immunosensor based on manganese dioxide-graphene nanoplatelets and core shell Fe3O4@Au nanoparticles for label-free detection of carcinoembryonic antigen.

A novel electrochemical immunosensor was developed for label-free detection of carcinoembryonic antigen (CEA) as a cancer biomarker. The designed immunosensor was based on CEA antibody (anti-CEA) anchored with core shell Fe3O4@Au nanoparticles which were immobilized on a screen-printed carbon electrode modified with manganese dioxide decorating on graphene nanoplatelets (SPCE/GNP-MnO2/Fe3O4@Au-antiCEA). The SPCE was placed onto a home-made electrode holder for easy handling. The approach was based on direct binding of CEA to a fixed amount of anti-CEA on the modified electrode for the specific detection using linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) monitored in a solution containing 5 mM [Fe(CN)63-/4-] prepared in 0.1 M phosphate buffer at pH 7.4. The difference in signal response owing to the redox reaction of [Fe(CN)6]3-/4- before and after interaction with CEA was regarded as the immunosensor response corresponding directly to the CEA concentration. Under optimized conditions, the linear range of 0.001-100 ng/mL, and the detection limits of 0.10 pg/mL (LSV) and 0.30 pg/mL (EIS) were evaluated. The applicability of the immunosensor was verified by well-corresponding determination of CEA in diluted human serum samples by electrochemiluminescence (ECL) immunoassay. Therefore, the proposed immunosensor could be suitable enough for a real sample analysis of CEA.

Flow-injection amperometric determination of glucose using a biosensor based on immobilization of glucose oxidase onto Au seeds decorated on core Fe3O4 nanoparticles.

An amperometric biosensor based on chemisorption of glucose oxidase (GOx) on Au seeds decorated on magnetic core Fe3O4 nanoparticles (Fe3O4@Au) and their immobilization on screen-printed carbon electrode bulk-modified with manganese oxide (SPCE{MnO2}) was designed for the determination of glucose. The Fe3O4@Au/GOx modified SPCE{MnO2} was used in a flow-injection analysis (FIA) arrangement. The experimental conditions were investigated in amperometric mode with the following optimized parameters: flow rate 1.7 mL min(-1), applied potential +0.38 V, phosphate buffer solution (PBS; 0.1 mol L(-1), pH 7.0) as carrier and 3.89 unit mm(-2) enzyme glucose oxidase loading on the active surface of the SPCE. The designed biosensor in FIA arrangement yielded a linear dynamic range for glucose from 0.2 to 9.0 mmol L(-1) with a sensitivity of 2.52 µA mM(-1) cm(-2), a detection limit of 0.1 mmol L(-1) and a quantification limit of 0.3 mmol L(-1). Moreover, a good repeatability of 2.8% (number of measurements n=10) and a sufficient reproducibility of 4.0% (number of sensors n=3) were achieved. It was found that the studied system Fe3O4@Au facilitated not only a simpler enzyme immobilization but also provided wider linear range. The practical application of the proposed biosensor for FIA quantification of glucose was tested in glucose sirup samples, honeys and energy drinks with the results in good accordance with those obtained by an optical glucose meter and with the contents declared by the producers.