RESUMO
The distribution of iron-carbon nanoparticles in FeC-DSPE-PEG-2000 modification (micellar particles with structure (Fe) core-carbon shell; PEG-based coating) is studied. The greater part of the nanoparticles accumulated in the spleen and liver, a small amount in the lungs, and the minimum amount in the thymus. The structural changes in the lymphoid organs were minor and involved only the microcirculatory bed. Analysis of the peripheral blood showed manifest anemia, thrombocytopenia, and leukocytosis.
Assuntos
Carbono/farmacocinética , Portadores de Fármacos/farmacocinética , Ferro/farmacocinética , Nanopartículas de Magnetita/química , Animais , Carbono/metabolismo , Amarelo de Eosina-(YS) , Hematoxilina , Histocitoquímica , Ferro/metabolismo , Fígado/efeitos dos fármacos , Fígado/ultraestrutura , Pulmão/efeitos dos fármacos , Pulmão/ultraestrutura , Fosfatidiletanolaminas/química , Polietilenoglicóis/química , Ratos , Ratos Wistar , Baço/efeitos dos fármacos , Baço/ultraestrutura , Timo/efeitos dos fármacos , Timo/ultraestrutura , Distribuição TecidualRESUMO
Interactions of bimodal (fluorescent and magnetic) nanoparticles with HeLa cells were studied. The nanoparticles, characterized by high magnetic moment and relaxing capacity, exhibited fluorescence sufficient for their use as labels in confocal microscopy and flow cytometry. Penetration of these nanoparticles into the cell depended on their surface charge: positively charged nanoparticles of this structure penetrated inside, while negatively charged particles were not found in the cells.
Assuntos
Corantes Fluorescentes/metabolismo , Nanopartículas de Magnetita/química , Nanocompostos/química , Pontos Quânticos/metabolismo , Transporte Biológico , Carbono/química , Permeabilidade da Membrana Celular , Sobrevivência Celular , Citometria de Fluxo , Corantes Fluorescentes/química , Células HeLa , Humanos , Ferro/química , Microscopia Confocal , Pontos Quânticos/química , Eletricidade Estática , Propriedades de SuperfícieRESUMO
We studied new magnetic nanocomposites consisting of a core(Fe) and carbon-shell inert to biological media. Iron-carbon nanoparticles circulate in the bloodstream for several minutes and are primarily accumulated in the liver; less intensive accumulation was found in the spleen and minimum in the lungs, kidneys, and heart. Accumulation of nanoparticles in the liver leads to the development of destructive processes and is accompanied by activation of compensatory-adaptive mechanisms. In the liver and spleen, structural changes are mild and mainly relate to changes in the microvasculature. In 6 months, the total content of nanoparticles in all tissues decreased due to their elimination from the body and the structure of the organs returned to normal.
