RESUMO
Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the serodiagnosis of human fascioliasis.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Fasciolíase/diagnóstico , Animais , Anticorpos Anti-Helmínticos/imunologia , Antígenos de Helmintos/imunologia , Bovinos , Fasciolíase/sangue , Fasciolíase/imunologia , Humanos , Testes Imunológicos/métodosRESUMO
We report a PCR-based assay for the detection of Enterocytozoon bieneusi. We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen.
Assuntos
DNA de Protozoário/análise , Fezes/parasitologia , Microsporida/genética , Microsporida/isolamento & purificação , Microsporidiose/diagnóstico , Reação em Cadeia da Polimerase/métodos , Animais , DNA de Protozoário/isolamento & purificação , Diarreia/diagnóstico , Diarreia/parasitologia , Eletroforese em Gel de Ágar , Filtração , Fixadores , Formaldeído , Humanos , Microsporidiose/imunologia , PapelRESUMO
Microsporidia are emerging as opportunistic pathogens in patients with acquired immunodeficiency syndrome (AIDS). Enterocytozoon bieneusi is the most commonly reported microsporidium that is detected in gastrointestinal specimens. This report describes an in situ hybridization technique with a 30-base specific synthetic DNA probe for detection of E bieneusi by light microscopy. Formalin-fixed paraffin-embedded duodenal biopsy specimens from three patients with AIDS, chronic diarrhea, and E bieneusi infection confirmed by electron microscopy were used in this study. Light microscopic examination after colorimetric detection allowed the identification of different stages of the pathogen's life cycle in the cytoplasm of enterocytes. No cross-reactivity was noted between the probe and human DNA. Our study underscores the applicability of a synthetic-labeled oligonucleotide for the detection and identification of E bieneusi in clinical samples.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , DNA de Protozoário/análise , Encephalitozoon/genética , Encefalitozoonose/diagnóstico , Hibridização In Situ/métodos , Enteropatias Parasitárias/diagnóstico , Infecções Oportunistas Relacionadas com a AIDS/parasitologia , Animais , Sondas de DNA/química , Duodeno/parasitologia , Duodeno/patologia , Encephalitozoon/ultraestrutura , Encefalitozoonose/parasitologia , Enteropatia por HIV/diagnóstico , Humanos , Técnicas Imunoenzimáticas , Enteropatias Parasitárias/parasitologia , Reação em Cadeia da PolimeraseRESUMO
Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique rRNA intergenic spacer could be amplified from all samples infected with E. bieneusi, but no amplification was seen by using DNA purified from samples with Septata intestinalis or other parasites and from negative control human cells. These results suggest that the PCR in stool samples may be a useful tool for the diagnosis of intestinal microsporidiosis in patients with AIDS.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS/parasitologia , Gastroenteropatias/complicações , Gastroenteropatias/parasitologia , Microsporida/genética , Microsporida/isolamento & purificação , Microsporidiose/complicações , Microsporidiose/parasitologia , Reação em Cadeia da Polimerase/métodos , Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , Animais , Sequência de Bases , Biópsia , Primers do DNA/genética , DNA de Protozoário/genética , DNA de Protozoário/isolamento & purificação , DNA Ribossômico/genética , DNA Ribossômico/isolamento & purificação , Duodeno/parasitologia , Estudos de Avaliação como Assunto , Fezes/parasitologia , Gastroenteropatias/diagnóstico , Humanos , Microscopia Eletrônica , Microsporida/ultraestrutura , Microsporidiose/diagnósticoRESUMO
The successful treatment of Taenia saginata taeniasis with mebendazole is herein reported. 41 subjects ascertained to have taeniasis based on the history of passage of gravid segments and positivity for Taenia egg were treated with 300 mg mebendazole b.i.d. for 3 days without need for fasting. No side-effects were observed. The drug acts as a taeniacide. The worms were expelled either as degenerated boluses or fragmented segments on the 2nd to the 4th day (Mean: 2.4 days) after the initial dose. 33 (84.6%) of the 41 subjects expelled the worm. A follow-up of all the subjects 2--3 months after treatment revealed that all those who expelled the worm following treatment and 6 of those who did not were negative for both Taenia egg and gravid segment, or a cure rate of 95%.
