RESUMO
We report a child with an unusual coronary bridge crossing over the left anterior descending (LAD), circumflex (CX), and right coronary artery (RCA). The bridges range from 0.8-1.2 mm depth. The patient presented with exercise-induced syncope and myocardial infarction (MI). She exhibited no syncope after medicine and exercise control.
Assuntos
Infarto do Miocárdio , Miocárdio , Criança , Angiografia Coronária , Vasos Coronários , Feminino , Humanos , Síncope/diagnóstico , Síncope/etiologiaRESUMO
Due to the incorporation of gold nanoparticles (AuNPs), previously reported AuNP-based FRET nanoflares still have some problems, such as non-negligible cytotoxicity and a time-consuming preparation procedure. In this communication, a novel AuNP-free FRET nanoflare for intracellular ATP imaging is developed based on a DNA nanostructure, which is self-assembled through cyclic U-type hybridization only involving a certain number of DNA strands.
Assuntos
Trifosfato de Adenosina/metabolismo , DNA/química , Transferência Ressonante de Energia de Fluorescência/métodos , Nanoestruturas/química , Trifosfato de Adenosina/análise , Corantes Fluorescentes/química , Ouro/química , Humanos , Células MCF-7 , Nanopartículas Metálicas/química , Microscopia Confocal , Mitocôndrias/metabolismo , Hibridização de Ácido NucleicoRESUMO
Endogenous miRNA expression patterns are extremely cell-type-specific, thereby offering high prediction accuracy for different cell identities. Here, a DNA-tetrahedron-based "AND" logic gate is utilized as a molecular device that recognizes dual-miRNA inputs through strand hybridization to activate a computation cascade that produces controlled singlet oxygen in live cells, resulting in the death of the target cell.
Assuntos
DNA/química , MicroRNAs/química , Oxigênio Singlete/metabolismo , Apoptose , Linhagem Celular , Computadores Moleculares , DNA/metabolismo , Desenho de Equipamento , Regulação da Expressão Gênica , Humanos , Lógica , MicroRNAs/metabolismo , Hibridização de Ácido Nucleico , Processos FotoquímicosRESUMO
In 2015, we proposed FRET nanoflares for intracellular molecular (RNA, H+, and K+) detection. To improve detection accuracy and achieve on-demand sensing, herein, we developed photocaged FRET nanoflares for spatiotemporal microRNA imaging in living cells. In other words, the probes will not work until they are exposed to UV light.
