Soil recalcitrant but not labile organic nitrogen mineralization contributes to microbial nitrogen immobilization and plant nitrogen uptake.

Soil organic nitrogen (N) mineralization not only supports ecosystem productivity but also weakens carbon and N accumulation in soils. Recalcitrant (mainly mineral-associated organic matter) and labile (mainly particulate organic matter) organic materials differ dramatically in nature. Yet, the patterns and drivers of recalcitrant (MNrec) and labile (MNlab) organic N mineralization rates and their consequences on ecosystem N retention are still unclear. By collecting MNrec (299 observations) and MNlab (299 observations) from 57 15N tracing studies, we found that soil pH and total N were the master factors controlling MNrec and MNlab, respectively. This was consistent with the significantly higher rates of MNrec in alkaline soils and of MNlab in natural ecosystems. Interestingly, our analysis revealed that MNrec directly stimulated microbial N immobilization and plant N uptake, while MNlab stimulated the soil gross autotrophic nitrification which discouraged ammonium immobilization and accelerated nitrate production. We also noted that MNrec was more efficient at lower precipitation and higher temperatures due to increased soil pH. In contrast, MNlab was more efficient at higher precipitation and lower temperatures due to increased soil total N. Overall, we suggest that increasing MNrec may lead to a conservative N cycle, improving the ecosystem services and functions, while increasing MNlab may stimulate the potential risk of soil N loss.

Warming-Induced Stimulation of Soil N2O Emissions Counteracted by Elevated CO2 from Nine-Year Agroecosystem Temperature and Free Air Carbon Dioxide Enrichment.

Globally, agricultural soils account for approximately one-third of anthropogenic emissions of the potent greenhouse gas and stratospheric ozone-depleting substance nitrous oxide (N2O). Emissions of N2O from agricultural soils are affected by a number of global change factors, such as elevated air temperatures and elevated atmospheric carbon dioxide (CO2). Yet, a mechanistic understanding of how these climatic factors affect N2O emissions in agricultural soils remains largely unresolved. Here, we investigate the soil N2O emission pathway using a 15N tracing approach in a nine-year field experiment using a combined temperature and free air carbon dioxide enrichment (T-FACE). We show that the effect of CO2 enrichment completely counteracts warming-induced stimulation of both nitrification- and denitrification-derived N2O emissions. The elevated CO2 induced decrease in pH and labile organic nitrogen (N) masked the stimulation of organic carbon and N by warming. Unexpectedly, both elevated CO2 and warming had little effect on the abundances of the nitrifying and denitrifying genes. Overall, our study confirms the importance of multifactorial experiments to understand N2O emission pathways from agricultural soils under climate change. This better understanding is a prerequisite for more accurate models and the development of effective options to combat climate change.

Root rot destabilizes the Sanqi rhizosphere core fungal microbiome by reducing the negative connectivity of beneficial microbes.

The stability of microbial communities, especially among core taxa, is essential for supporting plant health. However, the impacts of disease infection on the stability of rhizosphere fungal core microbiome remain largely unexplored. In this study, we delved into the effects of root rot infestation on the community structure, function, network complexity, and stability of Sanqi fungal core microbiomes, employing amplicon sequencing combined with co-occurrence network and cohesion analyses. Our investigation revealed that root rot disease led to a decrease in the α-diversity but an increase in the ß-diversity of the Sanqi fungal core microbiomes in the rhizosphere. Notably, Ilyonectria, Plectosphaerella, and Fusarium emerged as indicator species in the rhizosphere core microbiome of root rot-infected Sanqi plants, while Mortierella predominated as the dominant biomarker taxa in healthy soils. Additionally, root rot diminished the complexity and modularity of the rhizosphere networks by reducing the metrics associated with nodes, edges, degrees, and modularity. Furthermore, root rot resulted in a reduction in the proportion of negative connections in the network and the negative/positive cohesion of the entire core fungal microbiome. Particularly noteworthy was the observation that root rot infection destabilized the rhizosphere core fungal microbiome by weakening the negative connectivity associated with beneficial agents. Collectively, these results highlight the significance of the negative connectivity of beneficial agents in ensuring the stability of core microbial community.IMPORTANCERoot rot disease has been reported as the most devastating disease in the production process of artificial cultivated Sanqi ginseng, which seriously threatens the Sanqi industry. This study provides valuable insights into how root rot influences microbial relationships within the community. These findings open up opportunities for disease prevention and the promotion of plant health by regulating microbial interactions. In summary, the research sheds light on the ecological consequences of root rot on rhizosphere fungal microbiomes and offers potential strategies for managing soil-borne diseases and enhancing plant health.

Integrative knowledge-based nitrogen management practices can provide positive effects on ecosystem nitrogen retention.

Knowledge-based nitrogen (N) management provides better synchronization of crop N demand with N supply to enhance crop production while reducing N losses. Yet, how these N management practices contribute to reducing N losses globally is unclear. Here we compiled 5,448 paired observations from 336 publications representing 286 sites to assess the impacts of four common knowledge-based N management practices, including balanced fertilization, organic fertilization, co-application of synthetic and organic fertilizers, and nitrification inhibitors, on global ecosystem N cycling. We found that organic and balanced fertilization rather than N-only fertilization stimulated soil nitrate retention by enhancing microbial biomass, but also stimulated soil N leaching and emissions relative to no fertilizer addition. Nitrification inhibitors, however, stimulated soil ammonium retention and plant N uptake while reducing N leaching and emissions. Therefore, integrative application of knowledge-based N management practices is imperative to stimulate ecosystem N retention and minimize the risk of N loss globally.

The loss of microbial autotoxin degradation functions is associated with the decline of beneficial bacterial agents induced by phenolic acids.

Continuous cultivation of medicinal plants can disrupt the rhizosphere's microbial community. There is still a need to know about the beneficial bacterial community, their putative drivers, and the potential functions they may have. This study used different growth years of Sanqi ginseng (Panax notoginseng) with root rot to look at the beneficial microbial community structure, the function of microbial carbon source utilization, and the function of rhizosphere soil metabolism. The beneficial bacterial community changed and the relative abundance of beneficial agents was suppressed significantly with the successive Sanqi ginseng plantings. The carbon source utilization capacity and diversity increased significantly, whereas three autotoxin degradation-related pathways (biosynthesis of other secondary metabolites, metabolism of terpenoids and polyketides, and xenobiotics biodegradation and metabolism) were downregulated considerably with planting year extended. The changes in the beneficial agents were driven by the shifts in phenolic acid profiles, and the decline of beneficial microbes led to the loss of microbial autotoxin degradation functions. Overall, these results provide insight into beneficial microbes, microbial functions, phenolic acids, and their interactions, and these findings are essential for maintaining healthy and sustainable cultivation of Sanqi ginseng. IMPORTANCE Sanqi ginseng is a valuable perennial Chinese herb with various benefits for human health. However, continuous cultivation causes a high incidence of root rot disease, which leads to decreased yield and serious economic losses and ultimately impedes the sustainable development of Chinese medicine production. The significance of this study is to reveal the pattern of changes in beneficial bacteria and their related functions in root rot diseased rhizosphere with the successive planting years of Sanqi ginseng. This study found that the decline of beneficial bacterial agents mediated by phenolic acid profiles appears to be associated with the loss of microbial autotoxin degradation functions. This result may have new implications for deciphering the causes of Sanqi ginseng's continuous cropping obstacles.

Global patterns and controls of yield and nitrogen use efficiency in rice.

Factors influencing rice (Oryza sativa L.) yield mainly include nitrogen (N) fertilizer, climate and soil properties. However, a comprehensive analysis of the role of climatic factors and soil physical and chemical properties and their interactions in controlling global yield and nitrogen use efficiency (e.g., agronomic efficiency of N (AEN)) of rice is still pending. In this article, we pooled 2293 observations from 363 articles and conducted a global systematic analysis. We found that the global mean yield and AEN were 6791 ± 48.6 kg ha-1 season-1 and 15.6 ± 0.29 kg kg-1, respectively. Rice yield was positively correlated with latitude, N application rate, soil total and available N, and soil organic carbon, but was negatively correlated with mean annual temperature (MAT) and soil bulk density. The response of yield to soil pH followed the parabolic model, with the peak occurring at pH = 6.35. Our analysis indicated that N application rate, soil total N, and MAT were the main factors driving rice yield globally, while precipitation promoted rice yield by enhancing soil total N. N application rate was the most important inhibitor of AEN globally, while soil cation exchange capacity (CEC) was the most important stimulator of AEN. MAT increased AEN through enhancing soil CEC, but precipitation decreased it by decreasing soil CEC. The yield varies with climatic zones, being greater in temperate and continental regions with low MAT than in tropical regions, but the opposite was observed for AEN. The driving factors of yield and AEN were climatic zone specific. Our findings emphasize that soil properties may interact with future changes in temperature to affect rice production. To achieve high AEN in rice fields, the central influence of CEC on AEN should be considered.

Attribution and driving force of nitrogen losses from the Taihu Lake Basin by the InVEST and GeoDetector models.

Quantifying temporal and spatial changes in reactive nitrogen (Nr) losses from a watershed and exploring its main drivers are the key to watershed water quality improvements. Huge Nr losses continue to threaten the safety of the water environment in the Taihu Lake Basin (TLB). Here, the InVEST and GeoDetector models were combined to estimate Nr losses in the TLB from 1990 to 2020 and explore driving forces. Different scenarios for Nr losses were compared, showing that Nr loss peaked at 181.66 × 103 t in 2000. The key factors affecting Nr loss are land use, followed by elevation, soil, and slope factors, and their mean q-values were 0.82, 0.52, 0.51, and 0.48, respectively. The scenario analysis revealed that Nr losses increased under the business-as-usual and economic development scenarios, while ecological conservation, increased nutrient use efficiency, and reduced nutrient application all contribute to a reduction in Nr losses. The findings provide a scientific reference for Nr loss control and future planning in the TLB.

Effects of Solidago canadensis L. on mineralization-immobilization turnover enhance its nitrogen competitiveness and invasiveness.

The effects of exotic plants on soil nitrogen (N) transformations may influence species invasion success. However, the complex interplay between invasive plant N uptake and N transformation in soils remains unclear. In the present study, a series of 15N-labeled pot experiments were carried out with Solidago canadensis L. (S. canadensis), an invasive plant, and the Ntrace tool was used to clarify the preferred inorganic N form and its effects on soil N transformation. According to the results, nitrate-N (NO3--N) uptake rates by S. canadensis were 2.38 and 2.28 mg N kg-1 d-1 in acidic and alkaline soil, respectively, which were significantly higher than the ammonium-N (NH4+-N) uptake rates (1.76 and 1.56 mg N kg-1 d-1, respectively), indicating that S. canadensis was a NO3--N-preferring plant, irrespective of pH condition. Gross N mineralization rate was 0.41 mg N kg-1 d-1 in alkaline soil in the presence of S. canadensis L., which was significantly lower than that in the control (no plant, CK, 2.44 mg N kg-1 d-1). Gross autotrophic nitrification rate also decreased from 5.95 mg N kg-1 d-1 in the CK to 0.04 mg N kg-1 d-1 in the presence of S. canadensis in alkaline soil. However, microbial N immobilization rate increased significantly from 1.09 to 2.16 mg N kg-1 d-1, and from 0.02 to 2.73 mg N kg-1 d-1 after S. canadensis planting, in acidic and alkaline soil, respectively. Heterotrophic nitrification rate was stimulated in the presence of S. canadensis to provide NO3--N to support the N requirements of plants and microbes. The results suggested that S. canadensis can influence the mineralization-immobilization turnover (MIT) to optimize its N requirements while limiting N supply for other plants in the system. The results of the present study enhance our understanding of the competitiveness and mechanisms of invasion of alien plants.

Expanding agroforestry can increase nitrate retention and mitigate the global impact of a leaky nitrogen cycle in croplands.

The internal soil nitrogen (N) cycle supplies N to plants and microorganisms but may induce N pollution in the environment. Understanding the variability of gross N cycling rates resulting from the global spatial heterogeneity of climatic and edaphic variables is essential for estimating the potential risk of N loss. Here we compiled 4,032 observations from 398 published 15N pool dilution and tracing studies to analyse the interactions between soil internal potential N cycling and environmental effects. We observed that the global potential N cycle changes from a conservative cycle in forests to a less conservative one in grasslands and a leaky one in croplands. Structural equation modelling revealed that soil properties (soil pH, total N and carbon-to-N ratio) were more important than the climate factors in shaping the internal potential N cycle, but different patterns in the potential N cycle of terrestrial ecosystems across climatic zones were also determined. The high spatial variations in the global soil potential N cycle suggest that shifting cropland systems towards agroforestry systems can be a solution to improve N conservation.

Increasing Nitrogen Losses Due to Changing Food Consumption Patterns in Bayannur City, China.

Increasing urbanization and affluence have led to changes in food consumption patterns. The application of nitrogen (N) fertilizers ensures food security but also leads to environmental pollution due to N losses, through processes such as acidification, eutrophication, and greenhouse gas emissions. To clarify whether changes in food consumption patterns could increase N losses and to explore sustainable food system pathways, this study integrated the Chinese Food System Dashboard and the Nutrient Flows in Food Chains, Environment and Resources Use model to quantify and compare the link between food consumption and N losses in different agricultural regions using a case study of Bayannur City in the Yellow River Basin from 2000 to 2016. During the study period, Bayannur's food consumption pattern changed from a "high carbohydrate and pork pattern" to a "high fiber and herbivore pattern", which represents a shift from low to high N consumption. The per-capita food consumption decreased by 11.55% from 425.41 kg cap-1, whereas the per-capita N losses increased by 12.42% from 35.60 kg N cap-1. The average share of the plant-oriented and animal-oriented food supply in these losses was 53.39% and 46.61%, respectively. There were differences in the food consumption patterns and N losses in Bayannur's farming, farming-pastoral, and pastoral regions. The changes in N losses were most significant in the pastoral region. The N losses to the environment increased sharply by 112.33% from 22.75 g N cap-1 over the past 16 years. The low level of economic development in Bayannur resulted in a shift in the food consumption pattern to a high N consumption. Four measures to protect food security and reduce the food N cost were proposed: (1) increasing the wheat planting area and maintaining the existing corn one; (2) expanding the scale of high-quality alfalfa planting; (3) enhancing the area of oat grass and wheat replanting; and (4) using modern planting technology.

Water quality status response to multiple anthropogenic activities in urban river.

Water quality evaluation and degrading factors identification are crucial for predicting water quality evolution trends in an urban river. However, under the coupling of multiple factors, these targets face great challenges. The water quality status response to multiple anthropogenic activities in an urban river was evaluated and predicted based on comprehensive assessment methods and random forest (RF) model. We found that the distribution of each physicochemical parameter exhibits an obvious spatial clustering. The mean pollution level and trophic status of the urban river are medium pollution (water quality index = 59.79; Nemerow's pollution index = 2.00) and light eutrophication (trophic level index = 57.30). The water quality status is sensitive to anthropogenic activities, showing the following order of TLI and NPI values: residential district > industrial district > agricultural district and downtown > suburbs > countryside. According to the redundancy analysis, constructed land (F = 15.90, p < 0.01) and domestic sewage (F = 14.20, p < 0.01) evinced as the crucial factors that aggravated the water quality pollution level. Based on the simulation results of the RF model (variation explained = 94.91%; R2 = 0.978), improving domestic sewage treatment standards is the most effective measure to improve the water quality (increased by 40.3-49.3%) in residential and industrial districts. While in a suburban district, improving the domestic sewage collection rate has more effectively (23%) than those in the residential and industrial districts. Conclusively, reducing exogenous pollution input and improving domestic sewage treatment standards are vital to urban river restoration. Clinical trial registration Not applicable.

Deciphering the Synergies of Reductive Soil Disinfestation Combined with Biochar and Antagonistic Microbial Inoculation in Cucumber Fusarium Wilt Suppression Through Rhizosphere Microbiota Structure.

Application of reductive soil disinfestation (RSD), biochar, and antagonistic microbes have become increasingly popular strategies in a microbiome-based approach to control soil-borne diseases. The combined effect of these remediation methods on the suppression of cucumber Fusarium wilt associated with microbiota reconstruction, however, is still unknown. In this study, we applied RSD treatment together with biochar and microbial application of Trichoderma and Bacillus spp. in Fusarium-diseased cucumbers to investigate their effects on wilt suppression, soil chemical changes, microbial abundances, and the rhizosphere communities. The results showed that initial RSD treatment followed by biochar amendment (RSD-BC) and combined applications of microbial inoculation and biochar (RSD-SQR-T37-BC) decreased nitrate concentration and raised soil pH, soil organic carbon (SOC), and ammonium in the treated soils. Under RSD, the applications of Bacillus (RSD-SQR), Trichoderma (RSD-T37), and biochar (RSD-BC) suppressed wilt incidence by 26.8%, 37.5%, and 32.5%, respectively, compared to non-RSD treatments. Moreover, RSD-SQR-T37-BC and RSD-T37 caused greater suppressiveness of Fusarium wilt and F. oxysporum by 57.0 and 33.5%, respectively. Rhizosphere beta diversity and alpha diversity revealed a difference between RSD-treated and non-RSD microbial groups. The significant increase in the abundance, richness, and diversity of bacteria, and the decrease in the abundance and diversity of fungi under RSD-induced treatments attributed to the general suppression. Identified bacterial (Bacillus, Pseudoxanthomonas, Flavobacterium, Flavisolibacter, and Arthrobacter) and fungal (Trichoderma, Chaetomium, Cladosporium, Psathyrella, and Westerdykella) genera were likely the potential antagonists of specific disease suppression for their significant increase of abundances under RSD-treated soils and high relative importance in linear models. This study infers that the RSD treatment induces potential synergies with biochar amendment and microbial applications, resulting in enhanced general-to-specific suppression mechanisms by changing the microbial community composition in the cucumber rhizosphere.

Biochar application can mitigate NH3 volatilization in acidic forest and upland soils but stimulates gaseous N losses in flooded acidic paddy soil.

Biochar (BC) has attracted attention for carbon sequestration, a strategy to mitigate climate change and alleviate soil acidification. Most meta-analyses have insufficiently elaborated the effects of BC on soil N transformation so the practical importance of BC could not be assessed. In this study, a 15N tracing study was conducted to investigate the effects of BC amendment on soil gross N transformations in acidic soils with different land-use types. The results show that the BC amendment accelerated the soil gross mineralization rate of labile organic N to NH4+ (MNlab) (3 %-128 %) which was associated with an increase in total nitrogen. BC mitigated NH3 volatilization (VNH3) (52 %-99 %) in upland and forest soils due to NH4+/NH3 adsorption, while it caused higher gaseous N losses (NH3 and N2O) in flooded paddy soils. An important function was the effect of BC addition on NH4+ oxidation (ONH4). While ONH4 increased (4 %-19 %) in upland soils, it was inhibited (34 %-71 %) in paddy soils and did not show a response in forest soils. Overall, the BC amendment reduced the potential risk of N loss (PRL), especially in forest soils (82 %-98 %). This study also shows that the BC effect on soil N cycling is land-use specific. The suitability of practices including BC hinges on the effects on gaseous N losses.

Effects of Reductive Soil Disinfestation Combined with Liquid-Readily Decomposable Compounds and Solid Plant Residues on the Bacterial Community and Functional Composition.

Reductive soil disinfestation (RSD) incorporated with sole plant residues or liquid-readily decomposable compounds is an effective management strategy to improve soil health. However, the synthetic effects of RSD incorporated with liquid-readily decomposable compounds and solid plant residues on soil ecosystem services remain unclear. Field experiments were carried out to investigate the effects of untreated soil (CK), RSD incorporated with sawdust (SA), molasses (MO), and their combinations (SA + MO) on the bacterial community and functional composition. The results showed that RSD treatments significantly altered soil bacterial community structure compared to CK treatment. The bacterial community structure and composition in MO and SA + MO treatments were clustered compared to SA treatment. This was mainly attributed to the readily decomposable carbon sources in molasses having a stronger driving force to reshape the soil microbial community during the RSD process. Furthermore, the functional compositions, such as the disinfestation efficiency of F. oxysporum (96.4 - 99.1%), abundances of nitrogen functional genes, soil metabolic activity, and functional diversity, were significantly increased in all of the RSD treatments. The highest disinfestation efficiency and abundances of denitrification (nirS and nrfA) and nitrogen fixation (nifH) genes were observed in SA + MO treatment. Specifically, SA + MO treatment enriched more abundant beneficial genera, e.g., Oxobacter, Paenibacillus, Cohnella, Rummeliibacillus, and Streptomyces, which were significantly and positively linked to disinfestation efficiency, soil metabolic activity, and denitrification processes. Our results indicated that combining RSD practices with liquid-readily decomposable compounds and solid plant residues could effectively improve soil microbial community and functional composition.

High carbon resource diversity enhances the certainty of successful plant pathogen and disease control.

The host-associated microbiome highly determines plant health. Available organic resources, such as food for microbes, are important in shaping microbial community structure and multifunctionality. However, how using organic resources precisely manipulates the soil microbiome and makes it supportive of plant health remains unclear. Here, we experimentally tested the influence of carbon resource diversity on the microbial trophic network and pathogen invasion success in a microcosm study. We further explored how resource diversity affects microbial evenness, community functions, and plant disease outcomes in systems involving tomato plants and the in vivo soil microbiome. Increasing available resource diversity altered trophic network architecture, increased microbial evenness, and thus increased the certainty of successful pathogen control. By contrast, the invasion resistance effects of low resource diversity were less effective and highly varied. Accordingly, increases in the evenness and connection of dominant species induced by high resource diversity significantly contributed to plant disease suppression. Furthermore, high carbohydrate diversity upregulated plant immune system regulation-related microbial functions. Our results deepen the biodiversity-invasion resistance theory and provide practical guidance for the control of plant pathogens and diseases by using organic resource-mediated approaches, such as crop rotation, intercropping, and organic amendments.

Inhibition of Elevated Atmospheric Carbon Dioxide to Soil Gross Nitrogen Mineralization Aggravated by Warming in an Agroecosystem.

The response of soil gross nitrogen (N) cycling to elevated carbon dioxide (CO2) concentration and temperature has been extensively studied in natural and semi-natural ecosystems. However, how these factors and their interaction affect soil gross N dynamics in agroecosystems, strongly disturbed by human activity, remains largely unknown. Here, a 15N tracer study under aerobic incubation was conducted to quantify soil gross N transformation rates in a paddy field exposed to elevated CO2 and/or temperature for 9 years in a warming and free air CO2 enrichment experiment. Results show that long-term exposure to elevated CO2 significantly inhibited or tended to inhibit gross N mineralization at elevated and ambient temperatures, respectively. The inhibition of soil gross N mineralization by elevating CO2 was aggravated by warming in this paddy field. The inhibition of gross N mineralization under elevated CO2 could be due to decreased soil pH. Long-term exposure to elevated CO2 also significantly reduced gross autotrophic nitrification at ambient temperature, probably due to decreased soil pH and gross N mineralization. In contrast, none of the gross N transformation rates were affected by long-term exposure to warming alone. Our study provides strong evidence that long-term dual exposure to elevated CO2 and temperature has a greater negative effect on gross N mineralization rate than the single exposure, potentially resulting in progressive N limitation in this agroecosystem and ultimately increasing demand for N fertilizer.

Cover Plants-Mediated Suppression of Fusarium Wilt and Root-Knot Incidence of Cucumber is Associated With the Changes of Rhizosphere Fungal Microbiome Structure-Under Plastic Shed System of North China.

Cover crops are known to alleviate the adverse effects of continuous cropping by influencing plant health and changing host fungal-microbiome structures. However, insight into the shift of rhizomicrobiota composition and their effects on plant growth performance and resistance mechanism is still limited under plastic shed cultivation (PSC). Four leafy vegetable rotations namely spinach rotation (SR), non-heading Chinese cabbage rotation (NCCR), coriander rotation (CR), and leafy lettuce rotation (LLR) were used as cover crops in 7-years of continuous cucumber planted soil (CC). Their ecological impacts were studied for plant growth performance, replant diseases incidence rate, and rhizosphere fungal microbiome. Compared to CC, SR showed a highly suppressive effect on fusarium wilt, i.e., by 13.2% in the spring season, while NCCR decreased the root-knot nematode incidence rate by 8.9% in the autumn season. Such protective effects caused a significant increase of shoot and fruit biomass and thus sustained the fruit quality of cucumber. High-throughput sequencing revealed that the CR, SR, and NCCR treatments altered the fungal community composition by increasing the abundance of the beneficial fungal genera, decreasing pathogenic taxa, and fostering the saprotrophic and symbiotic functions. However, the relative abundance of most of the potentially pathogenic fungal genera increased in CC and LLR cropping. There were 8 potential pathogens and 10 beneficial or biocontrol fungi characterized. It was found that Paecilomyces, Chaetomium, Cladorrhinum, Zopfiella, Purpureocillium, and Metarhizium were the putative biocontrol microbes that positively affected plant growth and replanted diseases inhibition. The characterized Fusarium, Dactylonectria, Alternaria, Gibberella, and Aspergillus were the key pathogenic fungal agents found to be negatively associated with plant growth characters, suggesting that rhizomicrobiome may play an important role in the occurrence of disease incidence of cucumber plants. Considering the ecological potential of some cover plants, this study suggested that rotation with spinach, non-heading Chinese cabbage, or coriander can enhance rhizosphere immunity by triggering the development of plant-protective fungal microbiomes under plastic shed cucumber cultivation.

Global patterns of soil gross immobilization of ammonium and nitrate in terrestrial ecosystems.

Microbial nitrogen (N) immobilization, which typically results in soil N retention but based on the balance of gross N immobilization over gross N production, affects the fate of the anthropogenic reactive N. However, global patterns and drivers of soil gross immobilization of ammonium (INH4 ) and nitrate (INO3 ) are still only tentatively known. Here, we provide a comprehensive analysis considering gross N production rates, soil properties, and climate and their interactions for a deeper understanding of the patterns and drivers of INH4 and INO3 . By compiling and analyzing 1966 observations from 274 15 N-labelled studies, we found a global average of INH4 and INO3 of 7.41 ± 0.72 and 2.03 ± 0.30 mg N kg-1  day-1 with a ratio of INO3 to INH4 (INO3 :INH4 ) of 0.79 ± 0.11. Soil INH4 and INO3 increased with increasing soil gross N mineralization (GNM) and nitrification (GN), microbial biomass, organic carbon, and total N and decreasing soil bulk density. Our analysis revealed that GNM and GN were the main stimulators for INH4 and INO3 , respectively. The structural equation modeling showed that higher soil microbial biomass, total N, pH, and precipitation stimulate INH4 and INO3 through enhancing GNM and GN. However, higher temperature and soil bulk density suppress INH4 and INO3 by reducing microbial biomass and total N. Soil INH4 varied with terrestrial ecosystems, being greater in grasslands and forests, which have higher rates of GNM, than in croplands. The highest INO3 :INH4 was observed in croplands, which had higher rates of GN. The global average of GN to INH4 was 2.86 ± 0.31, manifesting a high potential risk of N loss. We highlight that anthropogenic activities that influence soil properties and gross N production rates likely interact with future climate changes and land uses to affect soil N immobilization and, eventually, the fate of the anthropogenic reactive N.

Soil dissolved organic matters mediate bacterial taxa to enhance nitrification rates under wheat cultivation.

Studies have shown that dissolved organic matters (DOMs) may affect soil nutrient availability to plants due to their effect on microbial communities; however, the relationships of soil DOM-bacterial community-N function in response to root exudates remains poorly understand. Here, we evaluated the DOM composition, bacterial taxonomic variation and nitrogen transformation rates in both acidic and alkaline soils, with or without the typical nitrate preference plant (wheat, Triticum aestivum L.). After 30 days' cultivation, DOM compositions such as sugars, amines, amino acids, organic acid, and ketone were significantly increased in soil with wheat vs. bare soil, and these compounds were mainly involved in nitrogen metabolism pathways. Soil core bacterial abundance was changed while bacterial community diversity decreased in response to wheat planting. Function prediction analysis based on FAPROTAX software showed that the bacterial community were significantly (p < 0.05) affiliated with nitrification and organic compound degradation. Additionally, db-RDA and VPA analysis suggested that the contribution of soil DOM to the variance of bacterial community was stronger than that of soil available nutrients. Furthermore, the N-transformation related bacteria like Burkholderiales and ammonia-oxidizing bacteria (AOB) were positively correlated with soil gross nitrification rate, confirming that the soil N transformation was enhanced in both acidic and alkaline soils. Our results provide insight into how soil DOM affects the community structure and function of bacteria to regulate the process of nitrogen transformation in plant-soil system.

Global Patterns and Drivers of Soil Dissimilatory Nitrate Reduction to Ammonium.

Dissimilatory nitrate reduction to ammonium (DNRA), the nearly forgotten process in the terrestrial nitrogen (N) cycle, can conserve N by converting the mobile nitrate into non-mobile ammonium avoiding nitrate losses via denitrification, leaching, and runoff. However, global patterns and controlling factors of soil DNRA are still only rudimentarily known. By a meta-analysis of 231 observations from 85 published studies across terrestrial ecosystems, we find a global mean DNRA rate of 0.31 ± 0.05 mg N kg-1 day-1, being significantly greater in paddy soils (1.30 ± 0.59) than in forests (0.24 ± 0.03), grasslands (0.52 ± 0.15), and unfertilized croplands (0.18 ± 0.04). Soil DNRA was significantly enhanced at higher altitude and lower latitude. Soil DNRA was positively correlated with precipitation, temperature, pH, soil total carbon, and soil total N. Precipitation was the main stimulator for soil DNRA. Total carbon and pH were also important factors, but their effects were ecosystem-specific as total carbon stimulates DNRA in forest soils, whereas pH stimulates DNRA in unfertilized croplands and paddy soils. Higher temperatures inhibit soil DNRA via decreasing total carbon. Moreover, nitrous oxide (N2O) emissions were negatively related to soil DNRA. Thus, future changes in climate and land-use may interact with management practices that alter soil substrate availability and/or soil pH to enhance soil DNRA with positive effects on N conservation and lower N2O emissions.