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2.
Eur J Clin Microbiol Infect Dis ; 43(7): 1453-1459, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38676856

RESUMO

We present our findings on interpatient transmission, epidemic control measures, and the outcomes of a series of ten critically ill burn patients who were either colonized or infected with carbapenem-resistant Acinetobacter baumannii (CRAB). None of the five infected patients achieved clinical cure, and all experienced relapses. Microbiological failure was observed in 40% of the infected patients. The isolated CRAB strains were found to carry blaOXA-23 and armA resistance genes. Despite the lack of clinical cure, all five infected patients survived and were discharged from the Burn Intensive Care Unit.


Assuntos
Infecções por Acinetobacter , Acinetobacter baumannii , Antibacterianos , Compostos Azabicíclicos , Carbapenêmicos , Ceftazidima , Surtos de Doenças , Combinação de Medicamentos , Unidades de Terapia Intensiva , Sulbactam , Acinetobacter baumannii/efeitos dos fármacos , Acinetobacter baumannii/genética , Acinetobacter baumannii/isolamento & purificação , Humanos , Infecções por Acinetobacter/tratamento farmacológico , Infecções por Acinetobacter/microbiologia , Infecções por Acinetobacter/epidemiologia , Antibacterianos/uso terapêutico , Antibacterianos/farmacologia , Masculino , Compostos Azabicíclicos/uso terapêutico , Compostos Azabicíclicos/farmacologia , Sulbactam/uso terapêutico , Sulbactam/farmacologia , Feminino , Pessoa de Meia-Idade , Adulto , Carbapenêmicos/farmacologia , Carbapenêmicos/uso terapêutico , Ceftazidima/uso terapêutico , Ceftazidima/farmacologia , Queimaduras/complicações , Queimaduras/microbiologia , Quimioterapia Combinada , Resultado do Tratamento , Idoso , Infecção Hospitalar/microbiologia , Infecção Hospitalar/tratamento farmacológico , Infecção Hospitalar/epidemiologia , Farmacorresistência Bacteriana Múltipla/genética , beta-Lactamases/genética , Unidades de Queimados
3.
Eur J Clin Microbiol Infect Dis ; 43(5): 1025-1029, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38472519

RESUMO

The diagnosis of long COVID often relies on symptoms post-COVID-19, occasionally lacking biological evidence. This case study illustrates how investigating long COVID uncovered an underlying bartonellosis through clinical metagenomics. Following mild COVID-19, a 26-year-old woman experienced persistent symptoms during 5 months, including axillary adenopathy. Pathological examination, 16 S rRNA PCR, and clinical metagenomic analysis were done on an adenopathy biopsy. The latter revealed Bartonella henselae DNA and RNA. Treatment with clarithromycin improved symptoms. This case underscores the relevance of clinical metagenomics in diagnosing hidden infections. Post-COVID symptoms warrant thorough investigation, and bartonellosis should be considered in polyadenopathy cases, regardless of a recent history of cat or flea exposures.


Assuntos
Bartonella henselae , COVID-19 , Metagenômica , Humanos , Feminino , Bartonella henselae/genética , Bartonella henselae/isolamento & purificação , Adulto , COVID-19/diagnóstico , COVID-19/complicações , Metagenômica/métodos , SARS-CoV-2/genética , Antibacterianos/uso terapêutico , Claritromicina/uso terapêutico
4.
J Antimicrob Chemother ; 79(3): 539-545, 2024 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-38197448

RESUMO

OBJECTIVES: Haemophilus parainfluenzae is an opportunistic pathogen causing respiratory tract infection and sexually transmitted diseases. The emergence of multidrug resistance in this species is particularly worrisome, especially since the recent description of CTX-M-15 ESBL-producing isolates in Spain. The aim of this study was to characterize a CTX-M-15-producing H. parainfluenzae clinical isolate, HP01, obtained from a urethral swab. METHODS: MICs were determined with gradient strips for this isolate. Hydrolysis assays were performed with the ß LACTA test. Genomic DNA from HP01 was subjected to Illumina and Oxford Nanopore sequencing to investigate the genetic environment of blaCTX-M-15. Phylogenetic analysis was performed with available H. parainfluenzae genomes from the NCBI database, including CTX-M-15 producers. RESULTS: HP01, an XDR isolate, was resistant to penicillin, third-generation cephalosporins, fluoroquinolones, macrolides, cyclines and co-trimoxazole and susceptible only to carbapenems and rifampicin. HP01 carried blaTEM-1, blaCTX-M-15, tet(M), catS and mef(E)/mel and harboured amino acid substitutions in PBP3, PBP5, GyrA, ParC and FolA implicated in resistance. Genomic analysis revealed that blaCTX-M-15 was carried by a Tn3-like transposon inserted into a novel integrative and conjugative element (ICE), ICEHpaSLS, present on the chromosome and belonging to the ICEHin1056 family described in Haemophilus influenzae. The tet(M)-MEGA element was also detected on the chromosome. No plasmid was found. The phylogenetic analysis showed that four H. parainfluenzae producing CTX-M-15 clustered in the same clade. CONCLUSIONS: Here we report the description of an XDR H. parainfluenzae producing blaCTX-M-15 isolated from a urethral swab. The blaCTX-M-15 gene was inserted into an ICE structure similar to those recently described in CTX-M-15 producers in Spain. The emergence of XDR H. parainfluenzae producing blaCTX-M-15 is a matter of great concern. Careful surveillance is required to prevent its spread.


Assuntos
Antibacterianos , Haemophilus parainfluenzae , Haemophilus parainfluenzae/genética , Filogenia , Antibacterianos/farmacologia , Substituição de Aminoácidos , beta-Lactamases/genética
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