RESUMO
TITLE: Un caso de mielopatia pediatrica por VIH.
Assuntos
Infecções por HIV/complicações , Doenças da Medula Espinal/etiologia , Criança , Humanos , Imageamento por Ressonância Magnética , Masculino , Doenças da Medula Espinal/diagnóstico por imagemRESUMO
A hexane extract from the leaves of Laurus novocanariensis was investigated for its activity on ethanol metabolism in Wistar rats. The extract could interfere with blood ethanol elevation, the induction of gastric alcohol dehydrogenase (ADH) and the reduction of erythrocyte catalase (CAT). The sesquiterpene lactones dehydrocostus lactone and costunolide were identified in the active extract, and might represent its active constituents. These results support the use of L. novocanariensis in the traditional Madeira Island medicine to protect against ethanol injury.
Assuntos
Etanol/sangue , Lactonas/farmacologia , Laurus , Extratos Vegetais/farmacologia , Substâncias Protetoras/farmacologia , Sesquiterpenos/farmacologia , Álcool Desidrogenase/metabolismo , Animais , Catalase/sangue , Etanol/metabolismo , Esvaziamento Gástrico/efeitos dos fármacos , Hemoglobinas/metabolismo , Lactonas/isolamento & purificação , Laurus/química , Masculino , Folhas de Planta , Substâncias Protetoras/isolamento & purificação , Ratos , Ratos Wistar/sangue , Ratos Wistar/metabolismo , Sesquiterpenos/isolamento & purificaçãoRESUMO
Present study reports on the general bioactivity of strictosamide and on its effects on Na(+),K(+)-ATPase and Mg(2+)-ATPase activities of Charles River male mouse. Strictosamide is the main glycoalkaloid of Sarcocephalus latifolius (Rubiaceae) leaves and roots, used as medicinal plant in folk medicine. In this work, we studied the in vitro effects of various concentrations of strictosamide (0.25, 0.5, 1 or 2 mg/mL) and the in vivo effects of single doses (50, 100 or 200 mg/kg, i.p.) of this compound on kidney and brain Na(+),K(+)-ATPase and Mg(2+)-ATPase activities. Results of general study showed that strictosamide is slightly toxic to Charles River mouse (LD(50)=723.17 mg/kg), producing CNS depression and kidney toxicity, but the exact mechanism of these effects could not be defined. Strictosamide inhibited the in vitro and in vivo Mg(2+)-ATPase activity on kidney but had nonsignificant effect on brain. Furthermore, strictosamide had nonsignificant in vitro and in vivo effect on kidney Na(+),K(+)-ATPase activity but produced an in vivo increase of Na(+),K(+)-ATPase activity of brain, these findings suggesting that strictosamine may be related to the induction of alpha(2) isoform of Na(+),K(+)-ATPase and may account for the folk use of Sarcocephalus latifolius root infusion on hypertension.