Morphological study of the integument and corporal skeletal muscles of two psammophilous members of Scincidae (Scincus scincus and Eumeces schneideri).

Sand deserts are common biotopes on the earth's surface. Numerous morphological and physiological adaptations have appeared to cope with the peculiar conditions imposed by sandy substrates, such as abrasion, mechanical resistance and the potential low oxygen levels. The psammophilous scincids (Lepidosauria) Scincus scincus and Eumeces schneideri are among those. S. scincus is a species frequently used to study displacement inside a sandy substrate. E. schneideri is a species phylogenetically closely related to S. scincus with a similar lifestyle. The aims of this study focus on the morphology of the integument and the muscular system. Briefly, we describe interspecific differences at the superficial architecture of the scales pattern and the thickness of the integument. We highlight a high cellular turnover rate at the level of the basal germinal layer of the epidermis, which, we suggest, corresponds to an adaptation to cutaneous wear caused by abrasion. We demonstrate the presence of numerous cutaneous holocrine glands whose secretion probably plays a role in the flow of sand along the integument. Several strata of osteoderms strengthen the skin. We characterize the corporal (M. longissimus dorsi and M. rectus abdominus) and caudal muscular fibers using immunohistochemistry, and quantify them using morphometry. The musculature exhibits a high proportion of glycolytic fast fibers that allow rapid burying and are well adapted to this mechanically resistant and oxygen-poor substrate. Oxidative slow fibers are low in abundance, less than 10% in S. scincus, but a little higher in E. schneideri.

Cytological analysis of integumentary and muscular adaptations in three sand-dwelling marine teleosts, Ammodytes tobianus (Ammodytidae), Gorgasia preclara (Congridae) and Heteroconger hassi (Congridae) (Teleostei; Actinopterygii).

Sandy bottoms are a ubiquitous environment found from sea bottoms to intertidal and freshwater zones. They are inhabited by many invertebrates and vertebrates which have developed morphological and physiological adaptations to sustain life under these particular conditions. Sandy habitats exhibit three potential constraints: abrasion, hypoxia and mechanical resistance. Here, three teleost species living in sandy environments were investigated: Ammodytes tobianus (Ammodytidae), Gorgasia preclara and Heteroconger hassi (Congridae). These teleost fishes were studied for their integument and muscular systems, which are potentially subject to sand abrasion and hypoxia, respectively. Based on histochemistry and transmission electron microscopy, we found the complex mucus system of G. preclara and H. hassi consists of two types of goblet cells and one type of sacciform cell. The secretions of both species are made of complex polysaccharides. In contrast, the scaly integument of A. tobianus has only a few goblet cells and no sacciform cells. We also highlighted, by immunohistochemistry, that the epidermal cell proliferation was much higher for this latter species, potentially resulting from the high rate of sand abrasion when A. tobianus buries itself quickly in the substrate. For all species, the major muscle fibre type was revealed by histoenzymology and corresponds to fast glycolytic fibres followed by intermediate fibres with slow fibres in the lowest proportion. Ammodytes tobianus possesses the highest fast fibre proportion (about 87% for A. tobianus and 75-78% for both garden eels). Our results provide new insights into the previously poorly studied teleost species, such as G. preclara, and allow us to highlight the complex skin histology of both garden eel species. Furthermore, the previously unknown muscle typing of these three species was determined.

Comparative study of the visual system of two psammophilic lizards (Scincus scincus &Eumeces schneideri).

Sand deserts are common biotopes on the earth's surface. Some specialized vertebrate species have colonized these ecological habitats by living buried in the sand. Among these so called psammophilic species are the Scincidae sand dune living species Scincus scincus and Eumeces schneideri. These two skinks share a relatively similar behavioral ecology by living buried in sand, almost all the time for S. scincus and at least for some part of the day for E. schneideri. The visual system of these two lizards was investigated by histological, immunohistochemical, Magnetic Resonance Imaging (MRI) and morphometric techniques. Both skink species exhibit a retina lacking fovea, composed predominantly of cones presenting two types of oil droplets (pale blue-green and colorless). Both species possess a subset of rod like-photoreceptors (about 1 rod for 30 cones) evidenced by anti-rhodopsin immunoreactivity. A ratio 1:1-1:2 between ganglion cells and photoreceptors points to a linear connection (photoreceptors/bipolar neurons/ganglion cells) in the retina and indicates that both skinks more likely possess good visual acuity, even in the peripheral retina. The MRI analysis revealed differences between the species concerning the eye structures, with a more spherical eye shape for S. scincus, as well as a more flattened lens. The relative lens diameter of both species seems to correspond to a rather photopic pattern. Beside the fact that S. scincus and E. schneideri have different lifestyles, their visual capacities seem similar, and, generally speaking, these two psammophilic species theoretically exhibit visual capacities not far away from non-fossorial species.