RESUMO
BACKGROUND: This study will be proposed for investigating the effects of high-quality nursing intervention (HQNI) on the psychological disorder in patients with gastric cancer during perioperative period (GC-PPP). METHODS: A cumulative search from inception up to the March 31, 2020 will be performed in the following databases: Cochrane Library, MEDLINE, EMBASE, Web of Science, VIP database, and China National Knowledge Infrastructure. We will search all potential studies from those electronic databases regardless their language and publication status. We will only consider randomized controlled trials (RCTs) for inclusion, which explores the effect of HQNI on the psychological disorder in patients with GC-PPP. Study identification, information extraction, and study quality appraisal will be independently and respectively done by 2 researchers. Any different opinions between 2 researchers will be disentangled by a third researcher after discussion. Cochrane risk of bias tool will be used for study quality assessment, and RevMan 5.3 software will be utilized for statistical analysis. RESULTS: This study will provide a high-quality synthesis of psychological disorder outcomes to evaluate the effects and safety of HQNI for patients with GC-PPP. CONCLUSION: The findings of this study will provide reference and evidence to appraise whether HQNI is an effective on the psychological disorder in patients with GC-PPP STUDY REGISTRATION NUMBER:: INPLASY202040080.
Assuntos
Transtornos Mentais/epidemiologia , Transtornos Mentais/enfermagem , Neoplasias Gástricas/epidemiologia , Neoplasias Gástricas/enfermagem , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto , Projetos de Pesquisa , Metanálise como AssuntoRESUMO
Mitochondrial inheritance in Fusarium zanthoxyli and F. continuum, two canker-inducing pathogens of prickly ash (Zanthoxylum bungeanum) in northern China, was investigated by genotyping ascospore progeny obtained from laboratory crosses. Polymorphic regions of the mitochondrial genomes (mitogenomes) that contained indels and single-nucleotide polymorphisms (SNPs) were identified via comparative analyses of the complete mitogenomes of the parents used in the intraspecific crosses. A reciprocal genetic cross of F. zanthoxyli NRRL 66714 × NRRL 66285, and a separate cross of F. continuum â NRRL 66286 × â NRRL 66218, revealed that mitochondria were only inherited from the maternal parent. In addition, the reciprocal cross demonstrated that mitochondrial inheritance is not linked to mating type. Gene order in the circular mitogenomes of the prickly ash pathogens was identical to that previously reported for other fusaria and members of the Hypocreales, except that the TRNL tRNAs were duplicated in F. zanthoxyli NRRL 66714. The genomes contained 14 polypeptide-encoding genes involved in oxidative respiration, one intron-encoded ribosomal protein (rps3) gene, two ribosomal RNA (rRNA) genes, and 26-28 tRNA genes. The F. zanthoxyli mitogenomes were 80.9 and 98.7 kb in length, whereas those of F. continuum were considerably shorter and nearly identical in length at 63.4 kb. The significant differences in mitogenome length were primarily due to variable numbers of introns and open reading frames (ORFs) encoding hypothetical proteins.
Assuntos
DNA Mitocondrial/genética , Fusarium/genética , Genes Mitocondriais , China , Cruzamentos Genéticos , DNA Mitocondrial/química , Genoma Mitocondrial , Genótipo , Técnicas de Genotipagem , Técnicas de Tipagem Micológica , Polimorfismo Genético , Análise de Sequência de DNA , Zanthoxylum/microbiologiaRESUMO
Fusarium zanthoxyli and F. continuum are sister taxa that are the etiological agents of canker disease of prickly ash (Zanthoxylum bungeanum) in northern China. These two pathogens, together with F. torreyae, the causal agent of canker disease of the critically endangered conifer Florida torreya (Torreya taxifolia) from northern Florida and southwestern Georgia, constitute a novel clade, the F. torreyae species complex. To assess their reproductive mode, a polymerase chain reaction (PCR) assay targeting the MAT1-1 and MAT1-2 idiomorphs was designed and validated, using MAT sequences mined from the whole-genome sequence of the three F. torreyae clade pathogens and several closely related fusaria. Results of the MAT idiomorph PCR assay indicated that isolates of the three pathogens were MAT1-1 or MAT1-2. When MAT1-1 and MAT1-2 isolates of each species were crossed on carrot agar, all of the F. zanthoxyli (N = 30) and F. continuum (N = 3) isolates tested were female fertile, yielding mature perithecia with viable ascospores. By comparison, only one pairing of the five different isolates of F. torreyae produced perithecia; however, the majority of the asci in this cross aborted or produced fewer than eight ascospores. Of the three temperatures tested (i.e., 22, 25, and 27 C), the optimal temperature for perithecium production was 22-25 C in F. zanthoxyli and 25 C in F. continuum and F. torreyae. Ascospore progeny from three separate crosses of F. zanthoxyli and F. continuum and one cross of F. torreyae were genotyped to assess whether they were the products of genetic recombination and sexual reproduction. Genotyping of 34-40 progeny from the F. zanthoxyli and F. continuum crosses confirmed that they were the products of sexual reproduction. However, only 36% of the progeny in the F. torreyae cross were recombinant, which was roughly half of the nonparental progeny expected with three markers segregating.
Assuntos
Fusarium/genética , Fusarium/fisiologia , Doenças das Plantas/microbiologia , Árvores/microbiologia , China , DNA Fúngico/genética , Evolução Molecular , Florida , Fusarium/patogenicidade , Genes Fúngicos Tipo Acasalamento , Genoma Fúngico , Genótipo , Georgia , Filogenia , Reação em Cadeia da Polimerase , Recombinação Genética , Reprodução , Esporos FúngicosRESUMO
Canker disease of prickly ash (Zanthoxylum bungeanum) has caused a decline in the production of this economically important spice in northern China in the past 25 y. To identify the etiological agent, 38 fungal isolates were recovered from symptomatic tissues from trees in five provinces in China. These isolates were identified by conducting BLASTN queries of NCBI GenBank and phylogenetic analyses of DNA sequence data from the nuclear ribosomal internal transcribed spacer region (ITS rDNA), a portion of the translation elongation factor 1-α (TEF1) gene, and genes encoding RNA polymerase II largest (RPB1) and second largest (RPB2) subunits. Results of these analyses suggested that 30/38 isolates belonged to two novel fusaria most closely related to the Florida torreya (Torreya taxifolia Arn.) pathogen, Fusarium torreyae in Florida and Georgia. These three canker-inducing tree pathogens form a novel clade within Fusarium here designated the F. torreyae species complex (FTOSC). BLASTN queries of GenBank also revealed that 5/38 isolates recovered from cankers represented an undescribed phylogenetic species within the F. solani species complex (FSSC) designated FSSC 6. Stem inoculations of three fusaria on Z. bungeanum resulted in consistent canker symptoms from which these three fusaria were recovered. The two novel fusaria, however, induced significantly larger lesions than FSSC 6. Herein, the two novel prickly ash pathogens are formally described as F. zanthoxyli and F. continuum.
Assuntos
Fusarium/classificação , Fusarium/isolamento & purificação , Doenças das Plantas/microbiologia , Zanthoxylum/microbiologia , China , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/genética , DNA de Plantas/química , DNA de Plantas/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Fusarium/genética , Dados de Sequência Molecular , Fator 1 de Elongação de Peptídeos/genética , Filogenia , RNA Polimerase II/genética , Análise de Sequência de DNARESUMO
The growth characteristics of Porites lutea skeleton in east sea area of Hainan Island were studied by CoralXDS software based on X-ray chronology. The growth parameters obtained included extension rate (ER), skeleton density (D), and calcification rate (CR). The results showed that ER varied from 0.49 to 1.10 cm·a-1 with an annual average of 0.76 cm·a-1, D varied from 1.11 to 1.35 g·cm-3 with an annual average of 1.22 g·cm-3, and CR varied from 0.55 to 1.41 g·cm-2·a-1 with an annual average of 0.94 g·cm-2·a-1. Statistical analyses indicated that sea surface temperature (SST) was the key environmental factor that controlled the growth characteristics, as it highly co-varied with ER and CR, less so with D. All of the three growth characteristics increased with the increase of SST. There were other factors that influenced the growth characteristics of the coral column, such as light, water salinity, and hydrodynamics, etc. In addition, typhoon and severe tropical storms also imposed a significant impact on the growth pattern of Porites lutea coral. The change in growth pattern of coral skeleton in east of Hainan Island was a response to complex climate fluctuation. Over the past century, SST of east Hainan Island dramatically increased at a rate of 0.15 â·(10 a)-1. The SST increase trend for the oceanic region could be divided into two stages, early 1940s and early 1980s. The human activities and global warming was the main causes for the increase of SST.
Assuntos
Antozoários/crescimento & desenvolvimento , Aquecimento Global , Animais , China , Ilhas , Oceanos e Mares , Água do Mar , TemperaturaRESUMO
In the title compound, [Ag(C(7)H(7)O(3)S)(C(13)H(14)N(2))](n), the Ag(I) ion is coordinated in a T-shape by two N atoms from two symmetry-related 1,3-bis-(pyridin-4-yl)propane ligands and one O atom from a p-toluene-sulfonate ligand, forming a one-dimensional zigzag chain along [001]. In the crystal, weak C-Hâ¯O hydrogen bonds and weak Agâ¯Ag inter-actions [3.2628â (5)â Å] are observed.