In depth metagenomic analysis in contrasting oil wells reveals syntrophic bacterial and archaeal associations for oil biodegradation in petroleum reservoirs.

Microbial biodegradation of hydrocarbons in petroleum reservoirs has major consequences in the petroleum value and quality. The identification of microorganisms capable of in-situ degradation of hydrocarbons under the reservoir conditions is crucial to understand microbial roles in hydrocarbon transformation and the impact of oil exploration and production on energy resources. The aim of this study was to profile the metagenome of microbial communities in crude oils and associated formation water from two high temperature and relatively saline oil-production wells, where one has been subjected to water flooding (BA-2) and the other one is considered pristine (BA-1). The microbiome was studied in the fluids using shotgun metagenome sequencing. Distinct microbial compositions were revealed when comparing pristine and water flooded oil wells in contrast to the similar community structures observed between the aqueous and oil fluids from the same well (BA-2). The equal proportion of archaea and bacteria together with the greater anaerobic hydrocarbon degradation potential in the BA-1 pristine but degraded reservoir contrasted with the predominance of bacteria over archaea, aerobic pathways and lower frequency of anaerobic degradation genes in the BA-2 water flooded undegraded well. Our results suggest that Syntrophus, Syntrophomonas, candidatus Atribacteria and Synergistia, in association with mainly acetoclastic methanogenic archaea of the genus Methanothrix, were collectively responsible for the oil biodegradation observed in the pristine petroleum well BA-1. Conversely, the microbial composition of the water flooded oil well BA-2 was mainly dominated by the fast-growing and putatively aerobic opportunists Marinobacter and Marinobacterium. This presumable allochthonous community introduced a greater metabolic versatility, although oil biodegradation has not been detected hitherto perhaps due to in-reservoir unfavorable physicochemical conditions. These findings provide a better understanding of the petroleum reservoir microbiomes and their potential roles in biogeochemical processes occurring in environments with different geological and oil recovery histories.

Microbial diversity in degraded and non-degraded petroleum samples and comparison across oil reservoirs at local and global scales.

Microorganisms have shown their ability to colonize extreme environments including deep subsurface petroleum reservoirs. Physicochemical parameters may vary greatly among petroleum reservoirs worldwide and so do the microbial communities inhabiting these different environments. The present work aimed at the characterization of the microbiota in biodegraded and non-degraded petroleum samples from three Brazilian reservoirs and the comparison of microbial community diversity across oil reservoirs at local and global scales using 16S rRNA clone libraries. The analysis of 620 16S rRNA bacterial and archaeal sequences obtained from Brazilian oil samples revealed 42 bacterial OTUs and 21 archaeal OTUs. The bacterial community from the degraded oil was more diverse than the non-degraded samples. Non-degraded oil samples were overwhelmingly dominated by gammaproteobacterial sequences with a predominance of the genera Marinobacter and Marinobacterium. Comparisons of microbial diversity among oil reservoirs worldwide suggested an apparent correlation of prokaryotic communities with reservoir temperature and depth and no influence of geographic distance among reservoirs. The detailed analysis of the phylogenetic diversity across reservoirs allowed us to define a core microbiome encompassing three bacterial classes (Gammaproteobacteria, Clostridia, and Bacteroidia) and one archaeal class (Methanomicrobia) ubiquitous in petroleum reservoirs and presumably owning the abilities to sustain life in these environments.

Particle Fluxes and Bulk Geochemical Characterization of the Cabo Frio Upwelling System in Southeastern Brazil: Sediment Trap Experiments between Spring 2010 and Summer 2012.

Physical and biogeochemical processes in continental shelves act synergistically in both transporting and transforming suspended material, and ocean dynamics control the dispersion of particles by the coastal zone and their subsequent mixing and dilution within the shelf area constrained by oceanic boundary currents, followed by their gradual settling in a complex sedimentary scenario. One of these regions is the Cabo Frio Upwelling System located in a significantly productive area of Southeastern Brazil, under the control of the nutrient-poor western boundary Brazil Current but also with a wind-driven coastal upwelling zone, inducing cold-water intrusions of South Atlantic Central Water on the shelf. To understand these synergic interactions among physical and biogeochemical processes in the Cabo Frio shelf, a series of four experiments with a total of 98 discrete samples using sediment traps was performed from November 2010 to March 2012, located on the 145 m isobath on the edge of the continental shelf. The results showed that lateral transport might be relevant in some cases, especially in deep layers, although no clear seasonal cycle was detected. Two main physical-geochemical coupling scenarios were identified: singular downwelling events that can enhance particles fluxes and are potentially related to the Brazil Current oscillations; and events of significant fluxes related to the intrusion of the 18°C isotherm in the euphotic zone. The particulate matter settling in the Cabo Frio shelf area seems to belong to multiple marine and terrestrial sources, in which both Paraiba do Sul River and Guanabara Bay could be potential land-sources, although the particulate material might subject intense transformation (diagenesis) during its trajectory to the shelf edge.