Construção do mapa de grávidas como ferramenta de controle no enfrentamento da covid-19 em belém-PA: relato de experiência / Construction of the pregnant women map as a control tool in the confrontation of covid-19 in the municipality of Belém-PA: an experience report / Construcción del mapa de embarazadas como herramienta de control en enfrentamiento-19 en el municipio de Belém-PA: informe de experiencia

Introdução:No início de 2020 a Organização Mundial da Saúde declarou o período pandêmico pelo novo coronavírus, SARS-COV-2, agente etiológico da Covid-19, o qual tem se propagado pelo mundo. Diante disso, faz-se necessário refletir sobre as complicações durante a gestação a fim de superar esses desafios que perpassam esse contexto. Objetivo:Possibilitar o controle das gestantes por meio da tecnologia educacional do tipo mapa de grávidas. Metodologia:Trata-se de um estudo descritivo, do tipo relato de experiência com abordagem qualitativa. Resultados:A ideia inicial identificava as grávidas de acordo como o sexo do bebê, sendo que a produção dessas grávidas se dava a partir da utilização da folha do Etil, Vinil e Acetato.Logo, a gravida da cor verde indicava que o profissional não tinha conhecimento do sexo do bebê, a rosa identificava o sexo feminino e a azul o sexo masculino, porém ao longo das oficinas, este método de identificação foi modificado.Conclusões:A experiência de vivenciar este projeto observando o empenho dos profissionais que compõe a equipe da unidade de saúde, como enfermeira, técnica de enfermagem, agentes comunitários de saúde e médico e dentistas em construir seu mapa e levar isso para sua unidade, foi bastante satisfatória, uma vez que, permite que os profissionaise discentes fortaleçam a autonomia do cuidar e consequentemente a prática laboral (AU).

Introduction:TheIn early 2020, the World Health Organization declared a pandemic period by the new coronavirus, SARS-COV-2, etiologic agent of Covid-19, which has been spreading around the world. Therefore, it is necessary to reflect on the complications during pregnancy and the importance of nurses' care in order to overcome these challenges that permeate this context. Objective:Permitir el control de las mujeres embarazadas a través de la tecnología educativa del tipo de mapa de las mujeres embarazadas. Methodology:This is a descriptive study, of the experience report type, with a qualitative approach. Results:The initial idea was to identify pregnant women according to the sex of the baby, and the production of these pregnant women was done through the use of theEthyl, Vinyl and Acetate. Thus, the green gravida indicated that the professional had no knowledge of the sex of the baby, pink identified the female sex and blue the male sex, but throughout the workshops, this method of identification was modified. Conclusions:The experience of living this project, observing the professionals' effort to build their map and take it to their unit, was very satisfying and enhanced the student's experience (AU).

A principios de 2020, la Organización Mundial de la Salud (OMS) declaró un periodo de pandemia por el nuevo coronavirus, SARS-COV-2, agente etiológico del Covid-19, que se ha extendido por todo el mundo. Por lo tanto, es necesarioreflexionar sobre las complicaciones durante el embarazo y la importancia de los cuidados de las enfermeras para superar estos retos que impregnan este contexto. Objetivo: Permitir el control de las mujeres embarazadas a través de la tecnología educativa del tipo de mapa de las mujeres embarazadas. Metodología: Se trata de un estudio descriptivo, del tipo informe de experiencias con un enfoque cualitativo. Resultados: La idea inicial era identificar a las embarazadas según el sexo del bebé, y la elaboración de estas embarazadas se hacía mediante el uso de la hoja de Etilo, Vinilo y Acetato. Así, el verde gravida indicaba que el profesional no tenía conocimiento del sexo del bebé, el rosa identificaba el sexo femenino y el azul el masculino, pero a lo largo de los talleres, este método de identificación se fue modificando. Conclusiones: La experiencia de vivir este proyecto observando el compromiso de los profesionales en la construcción de su mapa y llevándolo a su unidad, fue muy satisfactoria y mejoró la experiencia del estudiante (AU).

Acquiring Iron-Reducing Enrichment Cultures: Environments, Methods and Quality Assessments.

Lateritic duricrusts cover iron ore deposits and form spatially restricted, unique canga ecosystems endangered by mining. Iron cycling, i.e., the dissolution and subsequent precipitation of iron, is able to restitute canga duricrusts, generating new habitats for endangered biota in post-mining landscapes. As iron-reducing bacteria can accelerate this iron cycling, we aim to retrieve microbial enrichment cultures suitable to mediate the large-scale restoration of cangas. For that, we collected water and sediment samples from the Carajás National Forest and cultivated the iron-reducing microorganisms therein using a specific medium. We measured the potential to reduce iron using ferrozine assays, growth rate and metabolic activity. Six out of seven enrichment cultures effectively reduced iron, showing that different environments harbor iron-reducing bacteria. The most promising enrichment cultures were obtained from environments with repeated flooding and drying cycles, i.e., periodically inundated grasslands and a plateau of an iron mining waste pile characterized by frequent soaking. Selected enrichment cultures contained iron-reducing and fermenting bacteria, such as Serratia and Enterobacter. We found higher iron-reducing potential in enrichment cultures with a higher cell density and microorganism diversity. The obtained enrichment cultures should be tested for canga restoration to generate benefits for biodiversity and contribute to more sustainable iron mining in the region.

Fadiga por compaixão e estratégias de enfrentamento diante da finitude / Compassion fatigue and coping strategies before death / Fatiga por compasión y estrategias de afrontamiento ante la finitud

Resumo A fadiga por compaixão é uma ameaça à saúde mental de profissionais de saúde diante da dificuldade em manejar a empatia. Assim, buscou-se verificar na literatura científica a correlação entre a fadiga por compaixão e a atuação de profissionais em unidades hospitalares que lidam constantemente com a morte, considerando estratégias adotadas para autocuidado. Realizou-se revisão integrativa da literatura, que identificou 11 artigos, nas bases de dados MEDLINE e PubMed, publicados entre 2011 e 2021. Constatou-se que a fadiga por compaixão ocorre com maior frequência em profissionais que lidam direta e recorrentemente com a morte, principalmente quando medidas distanásicas são adotadas. As principais estratégias de adaptação psicológica detectadas foram discussão de casos entre equipes, momentos de lazer e apoio de colegas, espiritualidade e meditação, além de uma liderança construtiva. Ressalta-se a necessidade de maior aprofundamento e novas pesquisas diante da escassez de estudos sobre o tema, principalmente no Brasil.

Abstract Compassion fatigue threatens healthcare professionals' mental health in face of difficulties in managing empathy. This integrative review sought to verify the correlation between compassion fatigue and health professionals' performance in hospital units that frequently deal with death, considering the self-care strategies adopted. Bibliographic search conducted on the MEDLINE and PubMed databases retrieved 11 articles published between 2011 and 2021. Results show that compassion fatigue occurs frequently in professionals who deal directly and recurrently with death, especially when dysthanasia measures are adopted. Case discussion between teams, leisure time and peer support, spirituality and meditation as well as constructive leadership were the main psychological adaptation strategies identified. Further and in-depth research is needed given the scarcity of study on the topic, especially in Brazil.

Resumen La fatiga por compasión es una amenaza para la salud mental de los profesionales de la salud ante la dificultad para gestionar la empatía. Se buscó en la literatura científica la correlación entre la fatiga por compasión y el trabajo de los profesionales en unidades hospitalarias que lidian constantemente con la muerte considerando las estrategias adoptadas para el autocuidado. La revisión integradora de la literatura realizada identificó 11 artículos en las bases de datos MEDLINE y PubMed, publicados entre 2011 y 2021. La fatiga por compasión se presenta más en los profesionales que lidian directa y recurrentemente con la muerte, especialmente durante la adopción de medidas de distanasia. Como principales estrategias de adaptación psicológica destacan la discusión de casos entre equipos, el tiempo libre y apoyo de los compañeros, la espiritualidad y meditación, y el liderazgo constructivo. Son necesarias más investigaciones a fondo dados los escasos estudios, especialmente en Brasil.

Caracterização do tipo de comunicação materna utilizada durante interação mãe-criança / Characterization of the type of maternal communication used during mother-child interaction / Caracterización del tipo de comunicación materna utilizada durante la interacción madre-hijo

Introdução: A mãe pode ser definida como coautora no desenvolvimento da linguagem de seu bebê. Para isso, a fala materna apresenta características próprias expressas através da função comunicativa nos diversos momentos de interação com a criança. Objetivo: identificar e analisar formas de comunicação utilizadas pela mãe no processo de interação com a criança. Métodos: Realizou-se uma revisão integrativa de literatura, selecionando artigos científicos publicados entre 2011 e 2020, nas bases de dados SciELO e LILACS, que passaram por teste de relevância. A análise de dados foi: realizar a identificação dos artigos, das principais técnicas de coleta de dados aplicadas nos estudos e das formas de comunicação utilizadas pelas mães para comunicar-se com seu bebê, sendo divididas em comunicação verbal, não verbal e manhês. Resultados: Através da comunicação oral (91%), a mãe utiliza das funções comunicativas no momento de interação recorrendo principalmente a perguntas (80%), nomeações (50%), significado/descrever objeto (40%) e afirmações (30%). Quanto aos enunciados maternos, encontrou-se em sua maioria o emprego de elogios (30%) e como forma de direcionar a atenção (50%). Na comunicação não-verbal (73%), destacou-se a realização de gestos como apontar (63%), mostrar (38%) e representativos (38%), e nas expressões faciais, a prevalência foi pelo uso de sorrisos (38%) e olhares (38%). Observou-se o uso do manhês em 45% dos artigos. Conclusão: Conclui-se que por meio do processo de interação, de acordo com o observado nos resultados, a mãe contribuí para o desenvolvimento comunicativo-linguístico de seu filho, pois utiliza de diferentes meios para se comunicar com ele, principalmente o da comunicação verbal.

Introduction: The mother can be defined as a co-author in her baby's language development. For this, maternal speech has its own characteristics expressed through the communicative function in the different moments of interaction with the child. Objective: To identify and analyze forms of communication used by the mother in the process of interacting with the child. Methodology: An integrative literature review was performed by the selection of scientific articles published between 2011 and 2020, on SciELO and LILACS databases, that passed through relevance tests. The data analysis was: performed by the identification of the article, the main techniques of data collecting applied on studies, and the communication ways used by mothers to communicate with their babies, being divided by verbal, nonverbal, and motherese. Results: Through oral communication (91%), the mother uses communicative functions in the interaction moment, resorting mainly to questions (80%), nominations (50%), meaning/describing an object (40%), and affirmation (30%). As for maternal utterance, it has been found mostly the use of compliments (30%) and as a way to direct the attention (50%). In the nonverbal communication (73%), it was highlighted the performance of gestures like pointing (63%), showing (38%) and representative (38%), and at the facial expressions, the prevalence was the use of smiles (38%) and looks (38%). The use of motherese was observed in 45% of the articles. Conclusion:It is concluded that by the interaction process, according to what was observed on results, the mother contributes to her child's communicative-linguistic development, because she uses different ways to communicate with the child, mainly verbal communication.

Introducción: La madre puede definirse como coautora en el desarrollo del lenguaje de su bebé. Para eso, el habla materna tiene características proprias expresadas a través de la función comunicativa en los diferentes momentos de interacción con el niño. Objetivo: identificar y analizar las formas de comunicación utilizadas por la madre en el proceso de interacción con el niño. Métodos: Se realizó una revisión integradora de la literatura, por la selección de artículos científicos publicados entre 2011 y 2020, en las bases de datos SciELO y LILACS, los cuales pasaron por una prueba de relevancia. El análisis de los datos consistió en identificar los artículos, las principales técnicas de recolección de datos aplicadas en los estudios y las formas de comunicación utilizadas por las madres para comunicarse con su bebé, las cuales se dividieron en comunicación verbal, no verbal y maternés. Resultados: A través de la comunicación oral (91%), la madre utiliza las funciones comunicativas en el momento de la interacción, utilizando principalmente preguntas (80%), nominaciones (50%), significado/objeto descriptivo (40%) y declaraciones (30%). Ya las expresiones maternas, encontramos principalmente el uso de elogios (30%) y como unamanera de llamar la atención (50%). En la comunicación no verbal (73%) se destacaron los gestos como apuntar (63%), mostrar (38%) y representativo (38%), y en las expresiones faciales, la prevalencia fue el uso de sonrisas (38%) y miradas (38%). El uso de maternés se observó en el 45% de los artículos. Conclusión: Se concluye que mediante el proceso de interacción, de acuerdo a lo observado en los resultados, la madre contribuye al desarrollo comunicativo-lingüístico de su hijo, ya que utiliza diferentes medios para comunicarse con ella, especialmente la comunicación verbal.

Clinical Experience with Compression Taping to Treat Seroma After Breast Cancer Surgery: A Medical Device Clinical Study.

OBJECTIVE: To evaluate the safety of compressive taping in patients with seroma secondary to breast cancer surgery. METHODS: This study was a nonrandomized clinical study of medical devices applied to women indicated for seroma aspiration puncture after breast cancer surgery in a public reference hospital in Brazil. The intervention consisted of applying compressive taping over the seroma fluctuation area and maintained for 5 days. Evaluated outcomes comprised dermal changes caused by taping, subjective symptoms reported during the intervention period, and percent change in the punctured volume before and after the intervention. RESULTS: A total of 35 women (mean age, 56.7 ± 12.2 years) were included. Most presented with an advanced disease stage (≥IIB; 62.9%). Although a 28.6% incidence of dermal changes caused by taping was observed, an average reduction in the aspirated volume of -28.2 mL was identified (95% confidence interval, -48.3 to -8.0; P = .008). Treatment adherence was high (91.4%), and most patients reported satisfaction with the treatment (85.7%). CONCLUSIONS: The use of compressive taping on seroma areas can be considered safe, is well accepted by patients, and might be used as a noninvasive treatment option for seroma developing after breast cancer surgery.

Bacillus cereus Improves Performance of Brazilian Green Dwarf Coconut Palms Seedlings With Reduced Chemical Fertilization.

Coconut production in the Amazon requires the knowledge and development of sustainable technologies to alleviate the detrimental effects of inorganic chemical fertilizers and intensive farming practices. In this study, we investigated the effects of plant growth-promoting rhizobacteria (PGPR) isolated from coconut seedlings on nutrient use efficiency (NUE) and physiological mechanisms related to biomass accumulation of seedlings grown with reduced inorganic fertilizer levels. Of the 96 PGPR isolates tested on rice plants, the isolate Bacillus cereus (UFRABC40) was selected, as it resulted in the most significant gain in growth variables. In a commercial coconut tree nursery, we subjected seedlings to two treatments, both with seven replications: control 100% NPK chemical fertilizer (CF) and B. cereus + 50% NPK CF. The results indicated that the inoculation increased phytohormone levels [190% indole acetic acid (IAA), 31% gibberellic acid GA3, and 17% gibberellic acid GA4] and leaf gas exchange [48% by assimilation of CO2 (A), 35% stomatal conductance to water vapor (gs), 33% transpiration, and 57% instantaneous carboxylation efficiency] in leaves. Furthermore, growth parameters (shoot, root, and total dry weight, height, and diameter) and macro- and micronutrient levels (95% N, 44% P, 92% K, 103 Ca, 46% Fe, 84% B) were improved. Our results show the potential ability of strain Bacillus cereus UFRABC40 to promote the growth performance of coconut seedlings under decreased application of inorganic fertilizers. The application of microbial-based products in coconut seedling production systems improves plants' physiological performance and the efficiency of nutrient use.

Diallyl disulfide prevents cigarette smoke-induced emphysema in mice.

INTRODUCTION: Cigarette smoke (CS) is the main risk factor for the development of chronic obstructive pulmonary disease (COPD) and pulmonary emphysema. The use of antioxidants has emerged as a potential therapeutic strategy to treat airway inflammation and lung diseases. In the current study, we investigated the potential therapeutic impact of diallyl disulfide (Dads) treatment in a murine model of CS-induced emphysema. METHODS: C57BL/6 mice were exposed to CS for 60 consecutive days and treated with vehicle or Dads (30, 60 or 90 mg/kg) by oral gavage for the last 30 days, three times/week. The control group was sham-smoked and received vehicle treatment. All mice were euthanized 24 h after day 60; bronchoalveolar lavage (BAL) was performed and lungs were processed for further experimentation. Histological (HE stained sections, assessment of mean linear intercept (Lm)), biochemical (nitrite, superoxide dismutase (SOD), glutathione transferase (GST), and malondialdehyde (MDA) equivalents), and molecular biology (metalloproteinase (MMP) 12, SOD2, carbonyl reductase 1 (CBR1), nitrotyrosine (PNK), 4-hydroxynonenal (4-HNE), and CYP2E1) analyses were performed. RESULTS: Treatment with Dads dose-dependently reduced CS-induced leukocyte infiltration into the airways (based on BAL fluid counts) and improved lung histology (indicated by a reduction of Lm). Furthermore, CS exposure dramatically reduced the activity of the antioxidant enzymes SOD and GST in lung tissue and increased nitrite and MDA levels in BAL; these effects were all effectively counteracted by Dads treatment. Western blot analysis further confirmed the antioxidant potential of Dads, showing that treatment prevented the CS-induced decrease in SOD2 expression and increase in lung damage markers, such as CBR1, PNK, and 4-HNE. Furthermore, increased MMP12 (an important hallmark of CS-induced emphysema) and CYP2E1 lung protein levels were significantly reduced in mice receiving Dads treatment. CONCLUSION: Our findings demonstrate that treatment with Dads is effective in preventing multiple pathological features of CS-induced emphysema in an in vivo mouse model. In addition, we have identified several proteins/enzymes, including 4-HNE, CBR1, and CYP2E1, that are modifiable by Dads and could represent specific therapeutic targets for the treatment of COPD and emphysema.

Interactions between malnutrition, soil-transmitted helminthiasis and poverty among children living in periurban communities in Maranhao State, Northeastern Brazil.

Poverty, malnutrition and neglected tropical diseases such as soil-transmitted helminthiases (STHs) interact in a multi-causal feedback network. This study aimed to assess the relationships between STHs, income and nutritional status of children in impoverished communities in the city of Caxias, Maranhao State, Northeastern Brazil. A cross-sectional survey (n=259 children) was carried out with the collection of fecal samples and assessment of sociodemographic, anthropometric, dietary and sanitation data. Hookworm infection and ascariasis presented prevalence rates of 14.3% and 9.3%, respectively. The logistic regression analysis showed that hookworm infection was more frequent in males (odds ratio [OR]=3.43; 95% confidence interval [CI]=1.45-8.08), children aged 11-15 years old (OR=3.72; 95% CI=1.19-11.62), children living in poor families (OR=2.44; 95% CI=1.04-5.68) and those living in rented houses (OR=5.74; 95%CI=1.91-17.25). Concerning ascariasis, living in the Caldeiroes community (OR=0.01; 95%CI=0-0.17) and belonging to the 11-15 years age group (OR=0.21; 95%CI=0.04-1.02) were protection factors. Poor children have a significantly lower frequency of consumption of meat, milk, vegetables, tubers and fruits than not poor children. The frequent consumption of meat, milk and tubers was associated with significant higher values in the parameter height-for-age, whereas the consumption of meat and milk positively influenced the weight-for-age. The frequencies of stunting, underweight and wasting were 8.1%, 4.9% and 2.9%, respectively. The multivariate model demonstrated that stunting was significantly associated with economic poverty (OR=2.82; 95%CI=1.03-7.70) and low weight was associated with male sex (OR=6.43; 95% CI=1.35-30.68). In conclusion, the study describes the interactions between the dimensions of development represented by income, STHs and nutritional status revealing the importance of raising income levels to improve the living conditions of families in impoverished communities in Northeastern Brazil.

Effects of combined 5-HT2A and cannabinoid receptor modulation on a schizophrenia-related prepulse inhibition deficit in mice.

RATIONALE: Prepulse inhibition of the startle reflex (PPI) is disrupted in several psychiatric disorders including schizophrenia. Understanding PPI pharmacology may help elucidate the pathophysiology of these disorders and lead to better treatments. Given the advantages of multi-target approaches for complex mental illnesses treatment, we have investigated the interaction between receptors known to modulate PPI (5-HT1A and 5-HT2A) and the neuromodulatory endocannabinoid system. OBJECTIVES: To investigate serotonin and cannabinoid receptor (CBR) co-modulation in a model of PPI disruption relevant to schizophrenia METHODS: Male Swiss mice were pretreated with WIN 55,212-2 (CBR agonist), rimonabant (CB1R inverse agonist), 8-OH-DPAT (5-HT1A/7 agonist), and volinanserin (5-HT2A antagonist) or with a combination of a cannabinoid and a serotonergic drug. PPI disruption was induced by acute administration of MK-801. RESULTS: WIN 55,212-2 and rimonabant did not change PPI nor block MK-801-induced deficits. 8-OH-DPAT increased PPI in control mice and, in a higher dose, inhibited MK-801-induced impairments. Volinanserin also increased PPI in control and MK-801-treated mice, presenting an inverted U-shaped dose-response curve. Co-administration of either cannabinoid ligand with 8-OH-DPAT did not change PPI; however, the combination of volinanserin with rimonabant increased PPI in both control and MK-801-exposed mice. CONCLUSIONS: WIN 55,212-2 and rimonabant had similar effects in PPI. Moreover, serotonin and cannabinoid receptors interact to modulate PPI. While co-modulation of CBR and 5-HT1A receptors did not change PPI, a beneficial effect of 5-HT2A and CB1R antagonist combination was detected, possibly mediated through potentiation of 5-HT2A blockade effects by concomitant CB1R blockade.

Sex Differences in the Impact of Dietary Fiber on Pulmonary Responses to Ozone.

Ozone causes airway hyperresponsiveness, a defining feature of asthma. We have reported that the gut microbiome contributes to sex differences in ozone-induced airway hyperresponsiveness. Altering dietary fiber affects the gut microbiome. The purpose of this study was to determine the effects of dietary fiber on pulmonary responses to ozone and whether these effects differ by sex. We fed male and female mice fiber-free diets or diets enriched in one of two types of dietary fiber, cellulose and pectin, for 3 days before ozone exposure. Compared with control diets or pectin-enriched diets, cellulose-enriched diets attenuated ozone-induced airway hyperresponsiveness in male but not female mice. In contrast, fiber-free diets augmented responses to ozone in female but not male mice. Analysis of 16S rRNA sequencing of fecal DNA also indicated sex differences in the impact of dietary fiber on the gut microbiome and identified bacterial taxa that were associated with ozone-induced airway hyperresponsiveness. Our data suggest that microbiome-based therapies such as prebiotics may provide an alternative therapeutic strategy for air pollution-triggered asthma, but they indicate that such therapeutics may need to be tailored differently for males and females.

The interleukin-33 receptor contributes to pulmonary responses to ozone in male mice: role of the microbiome.

BACKGROUND: Interleukin-33 is released in the airways following acute ozone exposure and has the ability to cause airway hyperresponsiveness, a defining feature of asthma. Ozone causes greater airway hyperresponsiveness in male than female mice. Moreover, sex differences in the gut microbiome account for sex differences in this response to ozone. The purpose of this study was to determine whether there were sex differences in the role of interleukin-33 in ozone-induced airway hyperresponsiveness and to examine the role of the microbiome in these events. METHODS: Wildtype mice and mice genetically deficient in ST2, the interleukin-33 receptor, were housed from weaning with either other mice of the same genotype and sex, or with mice of the same sex but opposite genotype. At 15 weeks of age, fecal pellets were harvested for 16S rRNA sequencing and the mice were then exposed to air or ozone. Airway responsiveness was measured and a bronchoalveolar lavage was performed 24 h after exposure. RESULTS: In same-housed mice, ozone-induced airway hyperresponsiveness was greater in male than female wildtype mice. ST2 deficiency reduced ozone-induced airway hyperresponsiveness in male but not female mice and abolished sex differences in the response to ozone. However, sex differences in the role of interleukin-33 were unrelated to type 2 cytokine release: ozone-induced increases in bronchoalveolar lavage interleukin-5 were greater in females than males and ST2 deficiency virtually abolished interleukin-5 in both sexes. Since gut microbiota contribute to sex differences in ozone-induced airway hyperresponsiveness, we examined the role of the microbiome in these ST2-dependent sex differences. To do so, we cohoused wildtype and ST2 deficient mice, a situation that allows for transfer of microbiota among cage-mates. Cohousing altered the gut microbial community structure, as indicated by 16S rRNA gene sequencing of fecal DNA and reversed the effect of ST2 deficiency on pulmonary responses to ozone in male mice. CONCLUSIONS: The data indicate that the interleukin-33 /ST2 pathway contributes to ozone-induced airway hyperresponsiveness in male mice and suggest that the role of interleukin-33 is mediated at the level of the gut microbiome.

Chitosan hydrogels containing nanoencapsulated phenytoin for cutaneous use: Skin permeation/penetration and efficacy in wound healing.

Although phenytoin is an antiepileptic drug used in the oral treatment of epilepsy, its off-label use as a cutaneous healing agent has been studied in recent years due to the frequent reports of gingival hyperplasia after oral administration. However, the cutaneous topical application of phenytoin should prevent percutaneous skin permeation. Therefore, the aim of this study was to evaluate the in vitro skin permeation/retention and in vivo effects of nanocapsules and nanoemulsions loaded with phenytoin and formulated as chitosan hydrogels on the healing process of cutaneous wounds in rats. The hydrogels had adequate pH values (4.9-5.6) for skin application, drug content of 0.025% (w/w), and non-Newtonian pseudoplastic rheological behaviour. Hydrogels containing nanocapsules and nanoemulsions enabled improved controlled release of phenytoin and adhesion to skin, compared with hydrogels containing non-encapsulated phenytoin. In vitro skin permeation studies showed that phenytoin permeation to the receptor compartment, and consequently the risk of systemic absorption, may be reduced by nanoencapsulation without any change in the in vivo performance of phenytoin in the wound healing process in rats.

Challenges and potentialities of nursing work in street medical offices.

OBJECTIVE: to analyze elements of the nursing work process in the Street Medical Offices, highlighting the challenges and potentialities of care for homeless people. METHOD: this is an exploratory research of qualitative nature supported by the perspective of the health work process. The study was conducted through semi-structured interviews with nurses from the teams of the street medical offices at the city of Maceió and data were analyzed according to the content analysis technique, approaching issues related to the object, instruments and purposes of the nursing work process. RESULTS: the identified themes were: Need for health care in the context of social and health vulnerability; Strategic planning and teamwork as tools for organizing the work process; Purposes and products of work: guaranteeing the right to access and care. . Before a work object designed by serious health needs resulting from the social vulnerability of this population, nurses use different instruments in their work process: strategic planning, acting in multiprofessional team and valorization of the light technologies of reception and bonding. CONCLUSION: apart from the difficulties, the study presents a successful experience that explores the potentiality of sharing relationships of humanized care.

Leprosy in the elderly population of Alagoas / A hanseníase na população idosa de Alagoas

Objective: to describe the epidemiological profile of elderly persons with leprosy in the state of Alagoas. Method: a descriptive epidemiological study of leprosy cases among the elderly reported to the Disease Notification Information System between 2005 and 2015 was carried out. Results: a total of 896 cases were identified, with a predominance of 60-69 years old (60.5%), who were male (50.4%), had no schooling (34.8%), no source of income (54.1%), multibacillary forms of the disease (67.9%), and a high proportion of grade I (30.3%) and II (11.8%) disability at diagnosis. Hyperendemicity was noted during the survey period, with a mean detection rate of 29.48 cases per 100,000 inhabitants. Conclusion: leprosy is a growing disease among the elderly and early detection should be encouraged in this population to prevent the impairment of active aging.

Objetivo: descrever o perfil epidemiológico de idosos com hanseníase no estado de Alagoas. Método: trata-se de um estudo epidemiológico descritivo dos casos de hanseníase em idosos notificados no Sistema de Informação de Agravos de Notificação em Alagoas, no período de 2005 a 2015. Resultados: foram identificados 896 casos em que o perfil da amostra revelou predominância da faixa etária de 60 a 69 anos (60,5%), sexo masculino (50,4%), sem escolaridade (34,8%), nenhuma fonte de renda (54,1%), formas multibacilares (67,9%) e elevada proporção de grau I e II de incapacidade no momento do diagnóstico, (30,3%) e (11,8%) respectivamente. Destaca-se a hiperendemicidade no período, com taxa de detecção média de 29,48 casos por 100 mil habitantes. Conclusão: assim, percebe-se a hanseníase como enfermidade crescente no idoso, devendo-se incentivar ações de detecção precoce nessa população, a fim de prevenir o comprometimento do envelhecimento ativo.

Reconstituted spray-dried phenytoin-loaded nanocapsules improve the in vivo phenytoin anticonvulsant effect and the survival time in mice.

This study evaluated the in vivo anticonvulsant effect of a spray-dried powder for reconstitution containing phenytoin-loaded lipid-core nanocapsules. The effect of chitosan coating on redispersibility, gastrointestinal stability, and drug release from nanoparticles was evaluated during the development of the powders. Maltodextrin was used as adjuvant in the spray-drying process. Chitosan coating played an important role in redispersibility, and large particles (>100 µm) were obtained using the highest concentration of solids in the feed. However, after aqueous redispersion, volume-based particle size was reduced to about 1 µm. The release of nanoparticles from the surface of the spherical microagglomerates (roundness index = 0.75) was confirmed by SEM analysis. Powders reconstituted in water recovered partially the nanometric properties of the original suspensions and were stable for 24 h. Phenytoin-loaded chitosan-coated nanocapsules and their redispersed powders have good gastrointestinal stability, and are able to control drug release in simulated gastric and intestinal fluids. Besides that, the reconstituted powder containing chitosan-coated nanocapsules exhibited improved anticonvulsant activity against seizures induced by pilocarpine in mice, compared to the non-encapsulated drug, representing an important approach in anticonvulsant treatments for children and adults.

Área de preservação permanente em processo de revegetação com espécies arbóreas e adubos verdes / Permanent preservation area revegetated with tree species and green manures

RESUMO O objetivo principal do presente trabalho foi diagnosticar a situação da área em estudo, incluindo o corpo hídrico que é abastecido pelo Córrego Samambaia. Foram realizadas medições de altura e diâmetro das espécies arbóreas, durante 12 meses. Além disso, ocorreram coletas de amostras da água da represa para análise dos parâmetros: pH, condutividade, fósforo total e metais pesados. Nos meses de julho e dezembro de 2014, foram realizadas análises de turbidez, cor, demanda bioquímica de oxigênio (DBO) e oxigênio dissolvido (OD). Foram também realizadas análises físico-químicas do solo. No monitoramento realizado de setembro de 2013 a agosto de 2014, algumas plantas tiveram boa resposta em relação ao uso dos adubos verdes; porém, outras tiveram alta mortandade durante o estudo, como foi o caso do pequi. A competição entre espécies exóticas e nativas foi reduzida, o que aumentou, em geral, a sobrevivência das espécies nativas do bioma do Cerrado. Elementos tais como manganês não apresentaram diferença significativa em todos os tratamentos nos períodos de avaliação. Os valores de macro e microporos foram parecidos, mas a área apresentou diferenças quanto à textura do solo analisado. Quanto aos testes de resistência à penetração (RP) feitos em áreas com e sem adubo verde, os maiores valores foram encontrados na área sem as espécies leguminosas, onde o solo estava mais compactado. Parâmetros como condutividade elétrica da água apresentaram-se estáveis durante os 12 meses de estudo. Os resultados para OD e DBO estão fora do preconizado pela Resolução nº 357 do Conselho Nacional do Meio Ambiente.

ABSTRACT The main objective of this paper was to diagnose the situation of the study area, including the water body that is fueled by Córrego Samambaia. Measurements of height and diameter of tree species were made for 12 months. In addition, dam water samples were collected and the following parameters were analyzed: pH, conductivity, total phosphorus and heavy metals. In July and December of 2014, turbidity, color, biochemical oxygen demand (BOD) and dissolved oxygen (DO) analyzes were made. Physico-chemical analyses of soil were also carried out. Monitoring carried out from September 2013 to August 2014 showed some plants with good response in relation to the use of green manures, but others showed high mortality during the study, as the pequi. The competition between alien and native was reduced, which increased overall survival. Elements such as manganese showed no significant difference in all treatments during evaluation's period. Macro and micropores values were similar, but for texture, the area showed some differences. For penetration resistance tests (PR) made in an area with and without green manure, the largest PR values were found in the one without legumes species. Parameters such as electric conductivity were stable during the 12 months of study. The results for DO and BOD are outside the recommended by the Resolution 357 of the National Environmental Council.

Challenges and potentialities of nursing work in street medical offices / Desafios e potencialidades do trabalho de Enfermagem em Consultório na Rua / Desafíos y potencialidades del trabajo de Enfermería en Consultorio en la Calle

ABSTRACT Objective: to analyze elements of the nursing work process in the Street Medical Offices, highlighting the challenges and potentialities of care for homeless people. Method: this is an exploratory research of qualitative nature supported by the perspective of the health work process. The study was conducted through semi-structured interviews with nurses from the teams of the street medical offices at the city of Maceió and data were analyzed according to the content analysis technique, approaching issues related to the object, instruments and purposes of the nursing work process. Results: the identified themes were: Need for health care in the context of social and health vulnerability; Strategic planning and teamwork as tools for organizing the work process; Purposes and products of work: guaranteeing the right to access and care. . Before a work object designed by serious health needs resulting from the social vulnerability of this population, nurses use different instruments in their work process: strategic planning, acting in multiprofessional team and valorization of the light technologies of reception and bonding. Conclusion: apart from the difficulties, the study presents a successful experience that explores the potentiality of sharing relationships of humanized care.

RESUMO Objetivo: analisar elementos do processo de trabalho de enfermagem no Consultório na Rua, evidenciando os desafios e potencialidades do cuidado à pessoa em situação de rua. Método: trata-se de uma pesquisa exploratória de natureza qualitativa sustentada pela perspectiva do processo de trabalho em saúde, mediante entrevistas semiestruturadas com enfermeiros das equipes de Consultório na Rua do município de Maceió, analisadas segundo a técnica de análise de conteúdo, abordando questões relativas ao objeto, instrumentos e finalidades do processo de trabalho de enfermagem. Resultados: temas identificados: Necessidade de cuidado à saúde no contexto de vulnerabilidade social e de saúde; Planejamento estratégico e trabalho em equipe como instrumentos da organização do processo de trabalho; Finalidades e produtos do trabalho: garantindo o direito ao acesso e ao cuidado. O enfermeiro, diante de um objeto de trabalho desenhado por graves necessidades de saúde resultantes da vulnerabilidade social dessa população, utiliza instrumentos variados em seu processo de trabalho: planejamento estratégico, atuação em equipe multiprofissional e valorização das tecnologias leves de acolhimento e vínculo. Conclusão: afora as dificuldades, o estudo apresenta uma experiência exitosa que explora a potencialidade de relações solidárias de cuidado humanizado.

RESUMEN Objetivo: analizar elementos del proceso de trabajo de enfermería en el Consultorio en la Calle, evidenciando los desafíos y potencialidades del cuidado a la persona en situación de calle. Método: se trata de una investigación exploratoria de naturaleza cualitativa apoyada por la perspectiva del proceso de trabajo en salud, mediante entrevistas semi-estructuradas con enfermeros de los equipos de Consultorio en la Calle del municipio de Maceió, analizadas según la técnica de análisis de contenido, enfocando cuestiones relativas al objeto, instrumentos y finalidades del proceso de trabajo de enfermería. Resultados: temas identificados: Necesidad de cuidado a la salud en el contexto de vulnerabilidad social y de salud; Planeamiento estratégico y trabajo en equipo como instrumentos de la organización del proceso de trabajo; Finalidades y productos del trabajo: garantizando el derecho al acceso y al cuidado. El enfermero, frente a un objeto de trabajo diseñado por graves necesidades de salud resultantes de la vulnerabilidad social de esa población, utiliza instrumentos variados en su proceso de trabajo: planeamiento estratégico, actuación en equipo multi-profesional y valorización de las tecnologías leves de acogimiento y vínculo. Conclusión: fuera las dificultades, el estudio presenta una experiencia exitosa que explora la potencialidad de relaciones solidarias de cuidado humanizado.

Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean

Abstract This study aimed to evaluate the tolerance to salinity and temperature, the genetic diversity and the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of common bean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The isolates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at different temperatures (28 °C, 33 °C, 38 °C, 43 °C and 48 °C). Genotypic characterization was performed based on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbiotic efficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among 98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to temperature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbon sources studied. Clustering analysis based on the ability to use carbon sources and on the tolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropici H12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolates showed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About 20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobium reference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that two efficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercial inoculant for common bean in Brazil and must be assayed in field experiments.

Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean.

This study aimed to evaluate the tolerance to salinity and temperature, the genetic diversity and the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of common bean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The isolates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at different temperatures (28°C, 33°C, 38°C, 43°C and 48°C). Genotypic characterization was performed based on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbiotic efficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among 98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to temperature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbon sources studied. Clustering analysis based on the ability to use carbon sources and on the tolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropici H12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolates showed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About 20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobium reference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that two efficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercial inoculant for common bean in Brazil and must be assayed in field experiments.

Waardenburg syndrome type I: Dental phenotypes and genetic analysis of an extended family

BACKGROUND: The aim of this study was to describe the pattern of inheritance and the clinical features in a large family with Waardenburg syndrome type I (WS1), detailing the dental abnormalities and screening for PAX3 mutations. MATERIAL AND METHODS: To characterize the pattern of inheritance and clinical features, 29 family members were evaluated by dermatologic, ophthalmologic, otorhinolaryngologic and orofacial examination. Molecular analysis of the PAX3 gene was performed. RESULTS: The pedigree of the family, including the last four generations, was constructed and revealed non-consanguineous marriages. Out of 29 descendants, 16 family members showed features of WS1, with 9 members showing two major criteria indicative of WS1. Five patients showed white forelock and iris hypopigmentation, and four showed dystopia canthorum and iris hypopigmentation. Two patients had hearing loss. Dental abnormalities were identified in three family members, including dental agenesis, conical teeth and taurodontism. Sequencing analysis failed to identify mutations in the PAX3 gene. CONCLUSIONS: These results confirm that WS1 was transmitted in this family in an autosomal dominant pattern with variable expressivity and high penetrance. The presence of dental manifestations, especially tooth agenesis and conical teeth which resulted in considerable aesthetic impact on affected individuals was a major clinical feature. Clinical relevance: This article reveals the presence of well-defined dental changes associated with WS1 and tries to establish a possible association between these two entities showing a new spectrum of WS1