Initial Maximum Push-Rim Propulsion and Sprint Performance in Elite Men's Wheelchair Basketball.

OBJECTIVES: This study sought to report the reliability (intrasession) values of initial maximum push-rim propulsion (IMPRP) and sprint performance in elite wheelchair basketball (WB) players and to assess the involvement of strength in sprint capacity. METHODS: Fifteen Spanish international WB male players participated in this study. The maximum single wheelchair push from a stationary position (IMPRP) and the sprint performance (ie, 3, 5, and 12 m) of WB players were measured in this study. RESULTS: IMPRP mechanical outputs V, Vmax, P, Rel. P, F, and Rel. F variables presented high reliability values (intraclass correlation coefficient [ICC] ≥ .92; coefficient of variation [CV] ≤ 8.04 ± 7.37; standard error of measurement [SEM] ≤ 29.92), but the maximum strength variables Pmax, Rel. Pmax, Fmax, and Rel. Fmax (ICC ≥ .63; CV ≤ 13.19 ± 16.63; SEM ≤ 203.76) showed lower ICC values and by contrast higher CV and SEM values. The most substantial correlations were identified between maximum IMPRP values (ie, V, Vmax, P, Rel. P, F, and Rel. F) and sprint performance in 3 m (r ± confidence limits ≥ -0.74 ± 0.22, very large; R2 ≥ .55), 5 m (r ± confidence limits ≥ -0.72 ± 0.24, very large; R2 ≥ .51), and 12 m (r ± confidence limits ≥ -0.67 ± 0.27, large; R2 ≥ .44). CONCLUSIONS: The IMPRP test and sprint tests (3, 5, and 12 m) are practical and reliable for measuring strength and speed in WB players. In addition, there were large to very large associations among strength variables (ie, P, Rel. P, F, and Rel. F) and all sprint variables. This could indicate a need to implement specific strength exercises in WB players to improve sprint capacity.

The distribution of different intensity demanding scenarios in elite rink hockey players using an electronic performance tracking system.

Despite the traditional use of average values for determining physical demands, the intermittent and fluctuating nature of team sports may lead to underestimation of the most demanding scenarios. All the most demanding scenario-related investigations to date only report one maximal scenario per game, the greatest. However, the latest research on this subject has shown additional scenarios of equal or similar magnitude that most researchers have not considered. This repetition concept started a new way of describing competition and training loads; then the study aims were: first, to quantify and assess differences between playing positions in terms of the most demanding scenarios in official matches; and second, to quantify and assess the differences between playing positions in the repetition of different intensity scenarios relative to the most demanding individual scenario. We monitored nine professional rink hockey players (7 exterior and 2 interior players) in 18 competitive matches using an electronic performance tracking system. The interior players are closest to the opponent's goal, while the exterior players are farthest from it. Peak physical demands variables included total distance (m), distance covered at >18 km·h-1 (m), the number of accelerations (≥2 m∙s-2, count) and decelerations (≤-2 m∙s-2, count) in 30 s. An average from the top three individual most demanding scenarios was used to define a reference value to quantify the distribution scenario repetition during matches. The results showed that peak demands in rink hockey are position-dependent, with more distance covered by exterior players and more accelerations performed by interior players. In addition, rink hockey matches include multiple scenario exposures that are close to the peak physical demands of a match. Using the results of this study, coaches can prepare tailored training plans for each position, focusing on distances covered or accelerations for exterior players.

Initial Maximum Push-Rim Propulsion and Sprint Performance in Elite Women's Wheelchair Basketball: Differences Between Players' Functional Classification.

The aim of the present study was to determine, for the first time in elite women's wheelchair basketball, the differences in the initial maximum push-rim propulsion (IMPRP), mechanical outputs, and sprint performance between A category (≤2.5 class) and B category (≥3 class) players. A secondary aim was to assess the association between IMPRP mechanical outputs and sprint performance to determine the influence of specific strength in the latter. Sixteen Spanish women's wheelchair basketball players participated in this study: A category (n = 9, International Wheelchair Basketball Federation [IWBF] classification range: 1-2.5 points) and B category (n = 7, IWBF classification range: 3-4.5 points). All the players undertook 2 tests: the IMPRP test to measure the strength variables and the 3-, 5-, and 12-m sprint test. B category players reported significantly better performance values in almost all the IMPRP variables and all the sprint distances than A category players. Large to very large significant correlations were observed among IMPRP absolute mechanical outputs (excepting mean power) and sprint performance (3, 5, and 12 m) for all the participants. When the group was divided according to categories A and B, the significant differences observed in the whole group decreased in both groups, although some relationships were large or very large. The IMPRP test and sprint test (3, 5, and 12 m) seemed to be sensitive to differentiate the level between the 2 functional categories. In addition, there was a high association between strength and sprint variables, which shows that greater strength could improve sprint capacity.

Influence of upper-limb muscle strength on the repeated change of direction ability in international-level wheelchair basketball players.

This study aims 1) to compare repeated change of direction ability (rCODA) and bench press (BP) between low (A category, ≤2.5) and high (B category, ≥3.0) sport classes and 2) to analyse the relationships between rCODA and BP performance in a sample of wheelchair basketball (WB) players. Seventeen world-class WB players volunteered participated in this study. All the players undertook two tests: the repeated (x12) Modified Agility T-test (rMAT) to measure the rCODA and the movement velocity in a BP test. No significant differences were observed between categories in the rMAT and BP. For the total sample, BP variables with a mean propulsive velocity of 1 m·s-1 (V1LOAD) largely correlated with all the full rMAT outcomes (r>-0.625; p< 0.05). Having a better BP could be favourable to perform repeated efforts and this seems particularly relevant in fatigue conditions, and especially for the B category players.

The effects of COVID-19 lockdown on jumping performance and aerobic capacity in elite handball players.

The aim of this research was to analyse the capacity of a home-based training programme to preserve aerobic capacity and jumping performance in top-level handball players during the COVID-19 lockdown. Eleven top-level male handball players from the same team participated in the study. A submaximal shuttle run test and a counter-movement jump test were used to measure the players' aerobic fitness and lower limb explosive strength, respectively. A 9-week home-based training programme was followed during lockdown. Pre-test measurements were assessed before the pandemic on 29 January 2020 and ended on 18 May 2020. Moderate significant mean heart rate increases were found in the late stages of the submaximal shuttle run test after the lockdown (stage 5, 8.6%, P = 0.015; ES = 0.873; stage 6, 7.7%, P = 0.020; ES = 0.886; stage 7, 6.4%, P = 0.019; ES = 0.827). Moderate significant blood lactate increases were observed immediately after the submaximal shuttle run test following the lockdown (30.1%, P = 0.016; ES = 0.670). In contrast, no changes were found in jump performance. A structured home-based training programme during the COVID-19 lockdown preserved lower limb explosive strength but was an insufficient stimulus to maintain aerobic capacity in top-level handball players.

Monitoring external load in elite male handball players depending on playing positions.

Monitoring workload is critical for elite training and competition, as well as preventing potential sports injuries. The assessment of external load in team sports has been provided with new technologies that help coaches to individualize training and optimize their team's playing system. In this study we characterized the physical demands of an elite handball team during an entire sports season. Novel data are reported for each playing position of this highly strenuous body-contact team sport. Sixteen world top players (5 wings, 2 centre backs, 6 backs, 3 line players) were equipped with a local positioning system (WIMU PRO) during fourteen official Spanish first league matches. Playing time, total distance covered at different running speeds, and acceleration variables were monitored. During a handball match, wings cover the greater distance by high-speed running (> 5.0 m·s-1): 410.3 ± 193.2 m, and by sprint (> 6.7 m·s-1): 98.0 ± 75.4 m. Centre backs perform the following playing position that supports the highest speed intensities during the matches: high-speed running: 243.2 ± 130.2 m; sprint: 62.0 ± 54.2 m. Centre backs also register the largest number of high-intensity decelerations (n = 142.7 ± 59.5) compared to wings (n = 112.9 ± 56.0), backs (n = 105.2 ± 49.2) and line players: 99.6 ± 28.9). This study provides helpful information for professional coaches and their technical staff to optimize training load and individualize the physical demands of their elite male handball players depending on each playing position.

Integrating External and Internal Load for Monitoring Fitness and Fatigue Status in Standard Microcycles in Elite Rink Hockey.

The aims of this study were 3-fold: firstly, to present an integrative approach to external and internal load dynamics for monitoring fitness and fatigue status of specific in-court rink hockey training sessions in a standard microcycle; secondly, to assess the differences between training sessions and matches; the third and final aim was to assess the association between external and internal load metrics. The external load, using a local positioning system, and internal load, using the declared rate of perceived exertion, were measured during 23 in-season microcycles for nine top-level players. Training load data were analysed with regard to the number of days before or after a match [match day (MD) minus or plus]. In relation to the first aim, internal and external load metrics merged into a single integrated system using pooled data z-scores provided an invisible monitoring tool that places the players in the fitness-fatigue continuum throughout the different microcycle sessions. In this regard, MD-4 and MD-1 sessions tend to place, with a low dispersion, the players in a "low external and internal load" zone. On the contrary, in MD-3 and MD-2 sessions, as well as in MD, in which higher loads were recorded, most of the players were within a "high external and internal load" zone with a tendency towards dispersion towards the fitness or fatigue zones. Finally, and with regard to the second and third aims, an inverted "U-shape" load dynamic related to the specific goals of each training session was the main finding in terms of comparison between MD; a load peak between MD-3 and MD-2 sessions and a significant decrease in all the load variables in MD-1 sessions were found; and high-to-low correlations were found between external and internal load metrics. This study presents an integrative approach to the external and internal load of players for monitoring fitness and fatigue status during a standard microcycle in rink hockey that might provide team sport staff members with a deeper understanding of load distribution in the microcycle in relation to the match.

Hamstring Muscle Volume as an Indicator of Sprint Performance.

ABSTRACT: Nuell, S, Illera-Domínguez, V, Carmona, G, Macadam, P, Lloret, M, Padullés, JM, Alomar, X, and Cadefau, JA. Hamstring muscle volume as an indicator of sprint performance. J Strength Cond Res 35(4): 902-909, 2021-This study aimed to compare mechanical properties and performance during sprinting, as well as thigh muscle volumes (MVs), between national-level sprinters and physically active males. In addition, the relationships between thigh MVs and sprint mechanical properties and performance were investigated. Seven male sprinters and 9 actives performed maximal-effort 40-m sprints. Instantaneous velocity was measured by radar to obtain theoretical maximum force (F0), the theoretical maximum velocity (V0), and the maximum power (Pmax). For MV assessment, series of cross-sectional images of each subject's thigh were obtained by magnetic resonance imaging for each of the quadriceps and hamstring muscles and the adductor muscle group. Sprinters were faster over 10 m (7%, effect size [ES] = 2.12, p < 0.01) and 40 m (11%, ES = 3.68, p < 0.01), with significantly higher V0 (20%, ES = 4.53, p < 0.01) and Pmax (28%, ES = 3.04, p < 0.01). Sprinters had larger quadriceps (14%, ES = 1.12, p < 0.05), adductors (23%, ES = 1.33, p < 0.05), and hamstrings (32%, ES = 2.11, p < 0.01) MVs than actives. Hamstrings MV correlated strongly with 40-m sprint time (r = -0.670, p < 0.01) and V0 (r = 0.757, p < 0.01), and moderately with Pmax (r = 0.559, p < 0.05). Sprinters were significantly faster and had greater V0 and Pmax than active males. Larger MVs were found in sprinters' thighs, especially in the hamstring musculature, and strong correlations were found between hamstring MV and sprint mechanical properties and sprint performance.

Quantification of an Elite Futsal Team's Microcycle External Load by Using the Repetition of High and Very High Demanding Scenarios.

The main objective of this study was to describe the repetition of external load high-demanding scenarios and very high-demanding scenarios of match play for velocity, distance, and neuromuscular locomotor variables of an elite futsal team. Additionally, we also checked how these high- and very high-demanding scenarios were distributed throughout the microcycle. The most demanding scenario (measured using a rolling average method with a 1-min time window) of match play was measured out of thirteen elite futsal players using a local positioning system in the course of thirteen official matches and six in-season microcycles. A mean of the top three match play observations for each variable and each player were used to determine the most demanding scenario (100%) reference value. Data were reanalyzed to count the number of high-demanding scenarios (80-90% of the individual most demanding scenario) and very high-demanding scenarios (>90% of the individual most demanding scenario). The number of scenarios was analyzed with respect to the number of days prior to the match [match day (MD) minus X] and a bootstrap confidence interval approach was used to assess differences between MD. During a single match, players have to cope with repeated high- and very high-demanding scenarios. Moreover, the training session 2 days prior to the match was the one most similar to the match, surpassing it only in scenarios of locomotor velocity variables, albeit with significantly fewer scenarios of neuromuscular variables. The number of high- and very high-demanding scenarios in the training session prior to the match dropped significantly in comparison with the rest of the microcycle and the match. This new monitoring method may help practitioners to establish an accurate assessment of external load demands in competition and training.

Quantification of external load of elite rink hockey players in official matches.

BACKGROUND: The demands of elite rink hockey competition are unknown to coaches, although nowadays the explosion of tracking technology makes it possible to fill this research gap. The aims of this study were twofold: firstly, to describe the external load demands involved in a rink hockey match and secondly, to ascertain whether there were differences between exterior (EX) and interior or forward (IN) player positions. METHODS: A retrospective observational study was conducted between 2017 and 2018 competitive season. Eight professional players (6 EX and 2 IN) participated voluntarily in the study during 9 official matches. Data logging was performed with a local positioning system and the variables studied were distance covered (DT), distance covered above 18 km/h (HSS), player load (PL), number of high-intensity accelerations (ACC) and number of high-intensity decelerations (DEC). RESULTS: The average values per player were 4646±1135 m of DT for EX and 4919±1374 for IN, 897±252 m of HSS for EX and 981±313 for IN (mean ±SD); 173±69 and 167.5±57.5 ACC for EX and IN, respectively, and 164±56 and 152.5±42 DEC for EX and IN, respectively (median±IQR); and there were no significant differences (P>0.05), between positions. CONCLUSIONS: The findings of this study allow us to ascertain the external load in matches and that EX and IN did not present different physical demands. With this information, coaches can adjust training loads more accurately.

Hypertrophic muscle changes and sprint performance enhancement during a sprint-based training macrocycle in national-level sprinters.

Abstract This study aimed to analyse changes in sprint performance, muscle volumes (MVs) and sprint mechanical parameters (SMPs) in national-level sprinters performing a 5-month indoor sprint-based training macrocycle (SBTM). Twelve well-trained sprinters were tested on three different occasions throughout the SBTM. Testing procedures included: sprint performance over 10m, 40m, 80m, 150m, and 300m; MRI of thighs, to compute MVs of quadriceps, hamstrings and adductors; and a 40m sprint using a radar gun to assess SMPs such as theoretical maximal horizontal force, theoretical maximal horizontal velocity (V0), maximal power and index of force application (DRF). Improvements in sprint performance of between 4% and 7% (ES = 0.46-1.11, P < 0.01) were accompanied by increments in: quadriceps of 6% (ES = 0.41, P < 0.01), hamstrings of 10% (ES = 0.62, P < 0.01), adductors of 12% (ES = 0.87, P < 0.01), V0 of 5% (ES = 0.40, P < 0.01) and DRF of 7% (ES = 0.91, P < 0.01). In conclusion, during the SBTM after the off-season, moderate hypertrophic changes occur in sprinters. Moreover, the greater increase in hamstrings and adductors, compared with quadriceps, might be related to the prominent role of these muscle groups in sprinting. Furthermore, the SBTM was likely effective at developing sprint performance in sprinters, thereby endorsing the idea that sprint-specific training is crucial for highly trained individuals. Finally, our results support the notion that V0 or the "velocity-oriented" force-velocity profile is determinant of performance in sprinters.

Assessment of muscle fiber adaptation in footballers using a new ELISA assay of myosin isoforms.

BACKGROUND: To measure the impact of training models on injury incidence, data of health and performance were integrated to study fiber adaptation during a competitive season. We studied football players over a season, analyzing hours of exposure to sport by serum changes in fast and slow myosin, creatine kinase and lactate dehydrogenase. METHODS: A new assay was developed to measure the myosin isoforms in 49 non-sporting volunteers and in 27 professional football players. RESULTS: Myosin isoforms in volunteers with mean ages of 30±8 were 1553 µg/L fast and 1284 µg/L slow; in the group with of 56±7 were 1426 µg/L fast and 1046 µg/L slow. Slow myosin was significantly lower in older subjects (-18%). Samples from the players in preseason had lower mean scores for fast myosin (1123 µg/L) and higher for slow myosin (2072 µg/L) than reference volunteers. During the season, myosins reached the maximum with the maximum load (1537 µg/L fast, 2195 µg/L slow but decreased and adapted to the high level of demand (425 µg/L fast, 1342 µg/L slow). CK and LDH were maximal at the pre-season (227 U/L, 333 U/L) while myosin levels were maximal at the beginning of season (1537 µg/L, 2195 µg/L). CONCLUSIONS: Measuring serum myosin isoforms we identify the type and amount of damage caused by training and matches, making it a new control tool capable of advising training towards a minimum of blood slow myosin but controlling the fast fiber participating and be able to improve the performance of the players.

Sex differences in thigh muscle volumes, sprint performance and mechanical properties in national-level sprinters.

The purpose of this study was to determine and compare thigh muscle volumes (MVs), and sprint mechanical properties and performance between male and female national-level sprinters. We also studied possible relationships between thigh MVs and sprint performance. Nine male and eight female national-level sprinters participated in the study. T1-weighted magnetic resonance images of the thighs were obtained to determine MVs of quadriceps, hamstrings and adductors. Sprint performance was measured as the time to cover 40 and 80 m. Instantaneous sprint velocity was measured by radar to obtain theoretical maximum force (F0), theoretical maximum velocity (V0) and maximum power (Pmax). When MVs were normalized by height-mass, males showed larger hamstrings (13.5%, ES = 1.26, P < 0.05) compared with females, while quadriceps and adductors showed no statistically significant differences. Males were extremely faster than females in 40 m (14%, ES = 6.68, P < 0.001) and in 80 m (15%, ES = 5.01, P < 0.001. Males also showed increased sprint mechanical properties, with larger F0 (19%, ES = 1.98, P < 0.01), much larger Pmax (46%, ES = 3.76, P < 0.001), and extremely larger V0 (23%, ES = 6.97, P < 0.001). With the pooled data, hamstring and adductor MVs correlated strongly (r = -0.685, P < 0.01) and moderately (r = -0.530, P < 0.05), respectively, with sprint performance; while quadriceps showed no association. The sex-stratified analysis showed weaker associations compared with pooled data, most likely due to small sample size. In conclusion, males were faster than females and showed larger MVs, especially in hamstrings. Moreover, regarding the thigh muscles, hamstrings MV seems the most related with sprint performance as previously proposed.

Fibre-type-specific and Mitochondrial Biomarkers of Muscle Damage after Mountain Races.

Consequences of running mountain races on muscle damage were investigated by analysing serum muscle enzymes and fibre-type-specific sarcomere proteins. We studied 10 trained amateur and 6 highly trained runners who ran a 35 km and 55 km mountain trail race (MTR), respectively. Levels of creatine kinase (CK), CK-MB isoform (CK-MB), sarcomeric mitochondrial CK (sMtCK), transaminases (AST and ALT), cardiac troponin I (cTnI) and fast (FM) and slow myosin (SM) isoforms, were assessed before, 1 h, 24 h and 48 h after the beginning of MTR. Significant SM increases were found at 24 h in the 55 km group. Levels of CK, CK-MB, AST and cTnI were significantly elevated in both groups following MTR, but in the 55 km group they tended to stabilize in at 48 h. Using pooled data, time-independent serum peaks of SM and CK-MB were significantly correlated. Moreover, concentration of sMtCK was significantly elevated at 1 and 24 h after the race in the 35 km group. Although training volume could confer protection on the mitochondria, the increase in serum CK-MB and SM in the 55 km group might be related to damage to the contractile apparatus type I fibres. Competing in long-distance MTRs might be related to deeper type I muscle fibre damage, even in highly trained individuals.

Initial Maximum Push-Rim Propulsion and Sprint Performance in Elite Wheelchair Rugby Players.

García-Fresneda, A, Carmona, G, Padullés, X, Nuell, S, Padullés, JM, Cadefau, JA, and Iturricastillo, A. Initial maximum push-rim propulsion and sprint performance in elite wheelchair rugby players. J Strength Cond Res 33(3): 857-865, 2019-Wheelchair rugby (WR) is an increasingly popular Paralympic sport; however, the evidence base supporting the validity and reliability of field tests to assess the physical condition of WR players is in its infancy. Therefore, here, we aimed to evaluate the intrasession reliability of the initial maximum push-rim propulsion (IMPRP) test and the sprint test, and to determine the relationships between IMPRP mechanical outputs and sprint performance variables. We studied 16 Spanish WR players (aged 33 ± 9 years). The maximum single wheelchair push from a stationary position and the sprint performance (i.e., times for 3, 5, and 12 m, and the maximum velocity) of elite WR players were measured in this study. The intraclass correlation coefficient, coefficient of variation, and standard error of measurement for IMPRP variables were >0.85, <10.6%, and <16.76, respectively; the corresponding values for a linear sprint were >0.97, <3.50%, and <0.15. In relation to IMPRP mechanical outputs (i.e., acceleration, maximum acceleration, force, maximum force, power, and maximum power) and sprint performance (i.e., times for 3, 5, and 12 m, and the maximum velocity), significant and large associations were observed in the WR players (r ± confidence limit = -0.78 ± 0.17 to -0.90 ± 0.11; 0/0/100, most likely; R = 0.613-0.812; p < 0.001). These tests provide simple and reliable methods for obtaining accurate mechanical pushing capacities and sprint performances of WR competitors (the 61.4-80.1% variance in sprint performance was explained by the IMPRP variables). These relationships indicate a need to implement specific strength exercises in WR players with the aim of improving the IMPRP and therefore improving sprint capacity.

Early Functional and Morphological Muscle Adaptations During Short-Term Inertial-Squat Training.

Purpose: To assess early changes in muscle function and hypertrophy, measured as increases in muscle cross-sectional areas (CSAs) and total volume, over a 4 weeks inertial resistance training (RT) program. Methods: Ten young RT-naive volunteers (age 23.4 ± 4.1 years) underwent 10 training sessions (2-3 per week) consisting of five sets of 10 flywheel squats (moment of inertia 900 kg⋅cm2). Magnetic resonance imaging (MRI) scans of both thighs were performed before (PRE), and after 2 (IN) and 4 (POST) weeks of training to compute individual muscle volumes and regional CSAs. Scans were performed after ≥96 h of recovery after training sessions, to avoid any influence of acute muscle swelling. PRE and POST regional muscle activation was assessed using muscle functional MRI (mfMRI) scans. Concentric (CON) and eccentric (ECC) squat force and power, as well as maximal voluntary isometric contraction force (MVIC) of knee extensors and flexors, were measured in every training session. Results: Significant quadriceps hypertrophy was detected during (IN: 5.5% ± 1.9%) and after (POST: 8.6% ± 3.6%) the training program. Increases in squat force (CON: 32% ± 15%, ECC: 31 ± 15%) and power (CON: 51% ± 30%, ECC: 48% ± 27%) were observed over the training program. Knee extensor MVIC significantly increased 28% ± 17% after training, but no changes were seen in knee flexor MVIC. No correlation was found between regional muscular activation in the first session and the % of increase in regional CSAs (r = -0.043, P = 0.164). Conclusion: This study reports the earliest onset of whole-muscle hypertrophy documented to date. The process initiates early and continues in response to RT, contributing to initial increases in force. The results call into question the reliability of mfMRI as a tool for predicting the potential hypertrophic effects of a given strengthening exercise.

Time Course and Association of Functional and Biochemical Markers in Severe Semitendinosus Damage Following Intensive Eccentric Leg Curls: Differences between and within Subjects.

Purpose: To investigate the extent and evolution of hamstring muscle damage caused by an intensive bout of eccentric leg curls (ELCs) by (1) assessing the time course and association of different indirect markers of muscle damage such as changes in the force-generating capacity (FGC), functional magnetic resonance (fMRI), and serum muscle enzyme levels and (2) analyzing differences in the degree of hamstring muscle damage between and within subjects (limb-to-limb comparison). Methods: Thirteen male participants performed six sets of 10 repetitions of an ELC with each leg. Before and at regular intervals over 7 days after the exercise, FGC was measured with maximal isometric voluntary contraction (MVC). Serum enzyme levels, fMRI transverse relaxation time (T2) and perceived muscle soreness were also assessed and compared against the FGC. Results: Two groups of subjects were identified according to the extent of hamstring muscle damage based on decreased FGC and increased serum enzyme levels: high responders (n = 10, severe muscle damage) and moderate responders (n = 3, moderate muscle damage). In the high responders, fMRI T2 analysis revealed that the semitendinosus (ST) muscle suffered severe damage in the three regions measured (proximal, middle, and distal). The biceps femoris short head (BFsh) muscle was also damaged and there were significant differences in the FGC within subjects in the high responders. Conclusion: FGC and serum enzyme levels measured in 10 of the subjects from the sample were consistent with severe muscle damage. However, the results showed a wide range of peak MVC reductions, reflecting different degrees of damage between subjects (high and moderate responders). fMRI analysis confirmed that the ST was the hamstring muscle most damaged by ELCs, with uniform T2 changes across all the measured sections of this muscle. During intensive ELCs, the ST muscle could suffer an anomalous recruitment pattern due to fatigue and damage, placing an excessive load on the BFsh and causing it to perform a synergistic compensation that leads to structural damage. Finally, T2 and MVC values did not correlate for the leg with the smaller FGC decrease in the hamstring muscles, suggesting that long-lasting increases in T2 signals after FGC markers have returned to baseline values might indicate an adaptive process rather than damage.

Sarcomere Disruptions of Slow Fiber Resulting From Mountain Ultramarathon.

OBJECTIVE: To investigate changes after a mountain ultramarathon (MUM) in the serum concentration of fast (FM) and slow (SM) myosin isoforms, which are fiber-type-specific sarcomere proteins. The changes were compared against creatine kinase (CK), a widely used fiber-sarcolemma-damage biomarker, and cardiac troponin I (cTnI), a widely used cardiac biomarker. METHODS: Observational comparison of response in a single group of 8 endurance-trained amateur athletes. Time-related changes in serum levels of CK, cTnI, SM, and FM from competitors were analyzed before, 1 h after the MUM, and 24 and 48 h after the start of the MUM by 1-way ANOVA for repeated measures or Friedman and Wilcoxon tests. Pearson correlation coefficient was employed to examine associations between variables. RESULTS: While SM was significantly (P = .009) increased in serum 24 h after the beginning of the MUM, FM and cTnI did not change significantly. Serum CK activity peak was observed 1 h after the MUM (P = .002). Moreover, serum peaks of CK and SM were highly correlated (r = .884, P = .004). CONCLUSIONS: Since there is evidence of muscle damage after prolonged mountain running, the increase in SM serum concentration after a MUM could be indirect evidence of slow- (type I) fiber-specific sarcomere disruptions.