RESUMO
Comprobado el hecho de que los anestésicos utilizados en la practica odontológica, no tienen efectos adversos para la evolución normal del embarazo en la mujer, se elabora el presente trabajo realizado en 280 mujeres embarazadas que acudieron a la consulta prenatal, presentando diversas patologías odontológicas, especialmente emergencias odontológicas caracterizadas por dolor, las que luego del tratamiento bajo anestesia odontológica se comprobó que no presentaban ninguna alteración que pudiera amenazar la evolución normal del embarazo, resultado que recomienda la atención integral odontológica en la embarazada, sin el temor de amenazar su gestación.
Assuntos
Humanos , Cuidado Pré-Natal , Odontologia PreventivaRESUMO
A cytochrome c (NarC) is encoded as the first gene of the operon for nitrate respiration in Thermus thermophilus. NarC is required for anaerobic growth and for the synthesis of active nitrate reductase (NR). The alpha and delta subunits (NarG, NarJ) of the NR were constitutively expressed in narC::kat mutants, but NarG appeared in the soluble fraction instead of associated with the membranes. Our data demonstrate for NarC an essential role in the synthesis of active enzyme and for the attachment to the membrane of the respiratory NR from T. thermophilus.
Assuntos
Grupo dos Citocromos c/metabolismo , Complexos Multienzimáticos/metabolismo , NADH NADPH Oxirredutases/metabolismo , Nitrato Redutases/biossíntese , Thermus thermophilus/enzimologia , Grupo dos Citocromos c/genética , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Nitrato Redutase , Nitrato Redutases/genética , Nitrato Redutases/metabolismo , Óperon/genética , Thermus thermophilus/genética , Thermus thermophilus/metabolismoRESUMO
The presence of a periplasmic space within the cell envelope of Thermus thermophilus was analyzed in a mutant (HB8(Delta)UTR1) defective in the regulation of its S-layer (surface crystalline layer). This mutant forms round multicellular bodies (MBs) surrounded by a common envelope as the culture approaches the stationary phase. Confocal microscopy revealed that the origin of the MBs is the progressive detachment of the external layers coupled with the accumulation of NH(2)-containing material between the external envelopes and the peptidoglycan. A specific pattern of proteins was found as soluble components of the intercellular space of the MBs by a single fractionation procedure, suggesting that they are periplasmic-like components. To demonstrate this, we cloned a gene ( phoA) from T. thermophilus HB8 encoding a signal peptide-wearing alkaline phosphatase (AP), and engineered it to be overexpressed in the mutant from a shuttle vector. Most of the AP activity (>80%) was found as a soluble component of the MBs' intercellular fraction. All these data indicate that Thermus thermophilus actually has a periplasmic space which is functionally similar to that of Proteobacteria. The potential application of the HB8(Delta)UTR1 mutant for the overexpression of periplasmic thermophilic proteins is discussed.