RESUMO
Urban areas feature complex and heterogeneous land covers which create challenging issues for tree species classification. The increased availability of high spatial resolution multispectral satellite imagery and LiDAR datasets combined with the recent evolution of deep learning within remote sensing for object detection and scene classification, provide promising opportunities to map individual tree species with greater accuracy and resolution. However, there are knowledge gaps that are related to the contribution of Worldview-3 SWIR bands, very high resolution PAN band and LiDAR data in detailed tree species mapping. Additionally, contemporary deep learning methods are hampered by lack of training samples and difficulties of preparing training data. The objective of this study was to examine the potential of a novel deep learning method, Dense Convolutional Network (DenseNet), to identify dominant individual tree species in a complex urban environment within a fused image of WorldView-2 VNIR, Worldview-3 SWIR and LiDAR datasets. DenseNet results were compared against two popular machine classifiers in remote sensing image analysis, Random Forest (RF) and Support Vector Machine (SVM). Our results demonstrated that: (1) utilizing a data fusion approach beginning with VNIR and adding SWIR, LiDAR, and panchromatic (PAN) bands increased the overall accuracy of the DenseNet classifier from 75.9% to 76.8%, 81.1% and 82.6%, respectively. (2) DenseNet significantly outperformed RF and SVM for the classification of eight dominant tree species with an overall accuracy of 82.6%, compared to 51.8% and 52% for SVM and RF classifiers, respectively. (3) DenseNet maintained superior performance over RF and SVM classifiers under restricted training sample quantities which is a major limiting factor for deep learning techniques. Overall, the study reveals that DenseNet is more effective for urban tree species classification as it outperforms the popular RF and SVM techniques when working with highly complex image scenes regardless of training sample size.
Assuntos
Aprendizado Profundo , Máquina de Vetores de Suporte , Humanos , Redes Neurais de ComputaçãoRESUMO
A folate targeted camptothecin small molecule drug conjugate (SMDC) was synthesized using a monodisperse PEG spacer linked to folate via a releasable disulfide carbonate linker. Cell cytotoxicity in human KB cells exhibited an IC50 of 6nM. Importantly, activity of the prodrug was blocked by excess folate, demonstrating receptor-mediated celluar uptake of the PEG conjugate.