RESUMO
There are differences between children and adults in certain aspects of the Helicobacter pylori (HP) infection, among them the lower titre of IgG antibodies anti-HP in the former group. Thus, we investigated by means of flow cytometry CD4+/CD3+ (CD4+T), CD8+/CD3+ (CD8+T) and CD19+/CD3- (B) cells, activation/co-stimulatory markers (CD4+/HLA-DR+, CD4+/CD28+, CD8+/HLA-DR+ and CD8+/CD28+) and by means of ELISA IgG anti-HP antibodies in the peripheral blood from HP-positive and -negative children and adults. An increased CD4+/CD28+ and CD8+/CD28+ percentage and number of CD4+/CD3+ cells were seen in infected adults. Conversely, no difference was observed between infected and noninfected children, but when they were stratified by age, an increased CD4+/CD28+ cell percentage was seen in the HP-positive group older than 10 years. The mean level of IgG anti-HP was lower in younger infected children, increased with age and correlated with CD4+ cells. Our data suggest that the immune response to HP infection vary according to the age. Low percentage of activated CD4+ cell may contribute to the lower level of serum IgG anti-HP observed in younger infected children. In addition, the CD4+ cell participation during the infection seems to begin after 10 years old, when the immune response becomes similar to that seen in adults.
Assuntos
Anticorpos Antibacterianos/sangue , Linfócitos B/imunologia , Infecções por Helicobacter/imunologia , Helicobacter pylori , Subpopulações de Linfócitos/imunologia , Linfócitos T/imunologia , Adolescente , Adulto , Fatores Etários , Idoso , Antígenos CD/análise , Linfócitos B/classificação , Criança , Pré-Escolar , Feminino , Humanos , Imunoglobulina G/sangue , Imunofenotipagem , Masculino , Pessoa de Meia-Idade , Linfócitos T/classificaçãoRESUMO
The demonstration of the association of H, pylori with gastritis and peptic ulcer has been of increasing interest to gastroenterologists, microbiologists, and histopathologists. In this study, the presence of H, pylori in the gastric mucosa of children was investigated by culture, preformed urease test, and carbolfuchsin staining of biopsy smears. The organism was detected in 44,9% of the children studied, and found to be distributed equaly on the antral and fundic mucosa. Compared to culture, the urease test and carbolfuchsin staining proved to be of higher sensitivity and specificity in detecting H, pylori
Assuntos
Lactente , Pré-Escolar , Criança , Adolescente , Humanos , Masculino , Feminino , Infecções por Helicobacter/diagnóstico , Helicobacter pylori/isolamento & purificação , Mucosa Gástrica/microbiologia , Gastrite/etiologia , Infecções por Helicobacter/complicações , Úlcera Péptica/etiologiaRESUMO
1. Study of six patients with chronic calcifying pancreatitis (CCP) occurring over a 10 year period, representing an incidence rate of approximately 1:1,000 of the impatients in the children's ward of a general hospital in central Brazil, is reported. 2. Major clinical manifestations as well as therapeutic management are described. 3. The possible relationship between CCP and primary protein-calorie malnutrition is discussed and the importance of a CCP diagnosis when dealing malnourished children who do not respond satisfactorily to common therapy is emphasized