[Comparative analysis of multifollicular development with the application of recombinant FSH vs. urinary FSH in the results of in vitro fertilization]. / Análisis comparativo del desarrollo multifolicular con la aplicación de FSH recombinante vs. FSH urinaria en los resultados de fertilización in vitro.

The interactive function between intrafollicular factors related with ovum competence and embryo development played an important role getting high quality embryos during the embryo transference in in-vitro fertilization programs. Urinary gonadotropins are used widely to get homogeneous follicles during ovarian stimulation. Recombinant technology has been applied in these compounds to correlate with a best egg formation and higher pregnancy rates. We developed a prospective, linear and comparative study to aim the ovum/embryo development using urinary and recombinant FSH. A total of 100 patients were included in the study, 56 patients received rFSH (group I) and 44 hMG (group II). There were not differences in age, weight and body mass index as well as ovarian reserve. However, patients who used urinary gonadotrophins required a higher number of ampoules [31.7 +/- 8.6 vs. 20.7 +/- 6.4 (p < 0.001)]. No differences in peak E2, day of hCG and endometrial thickness. Although, no differences in number of egg mature, the fertilization rate was higher in the recombinant group [5.9 +/- 3.7 vs. 3.4 +/- 2.3 (p < 0.02)]. A higher number of embryo transfer were observed in the same group (3.4 +/- 1.7 vs. 1.9 +/- 2.2 (p < 0.004)]. The pregnancy rates were 34.3% and 29.6% for each group respectively.

[Influence of intrafollicular factors at ovarian level during oocyte/embryo development]. / Influencia de los factores intrafoliculares a nivel ovárico durante el desarrollo óvulo/embrionario.

There is series of factors associated to fertilization and embryo development events. Each factor has a role in the inter-relationship between various molecular events related to oocyte/embryo quality. Dynamic features associated to endocrine-paracrine environment are determinant to the role of each factor in ovaric follicullar development. Growth factors, vascular-endothelial growth factor, nitric oxide, leptin and others correlate in ovular maturity process. That is why growth factors, vascular-endothelial growth factor, nitric oxide, leptin and others can be expressed as embryo viability and implantation biomarkers.

[Embryo transfer: a critical step in assisted reproduction]. / Transferencia embrionaria: un paso crítico en reproducción asistida.

The advances and development of new procedures and techniques related to embryo development, and its final outcome, the embryo transfer; have increased significantly the pregnancy rate through this reproductive assisted technique. The embryo transfer is probably the last part in in-vitro fertilization programs and the success should be determined through the technical approach and procedure's characteristics. The use and standardization of the current techniques during the embryo transfer process allow increasing the success in pregnancy and implantation rates in a short period of time in the current Assisted Reproduction Programs.

[A prospective randomized trial of the impact of misoprostol (PgE1) on pregnancy rate after intrauterine insemination (IUI) therapy: a preliminary report]. / Estudio prospectivo y aleatorio del impacto de misoprostol (PgE1) en las tasas de embarazo con inseminación intrauterina (IIU): informe preliminar.

Although the use of prostaglandin plays an important role in the reproductive human physiology, it is still controversial in the reproductive field. Ovarian stimulation as well as intrauterine insemination increased the reproductive goals in certain group of patients. The objective of the present study was to evaluate the prostaglandin effect (misoprostol) in patients under ovarian stimulation and intrauterine insemination and their final outcome in the clinical pregnancy rate. There were a total of 59 ovarian stimulated cycles, the study group (n = 29) received 200 micrograms of prostaglandin E1 (misoprostol) intravaginal after IUI, compared with the control group (n = 30). Demographic characteristics were similar in both groups. There were no differences in age, FSH. LH and E2, hCG day and number of ampoules between groups. However, a significant pregnancy rate was observed between groups (31% study group vs. 20% control group). We concluded that prostaglandin application in stimulated cycles under intrauterine insemination remain a beneficial effect showing in the pregnancy outcome.

Infeccion genital por Chlamydia trachomatis. / Genital infectious by Chlamydia trachomatis

Se presenta una revision sobre la participacion y transcendencia de Chlamydia trachomatis en las infecciones del aparato genital, esfera en donde ha despertado interes creciente en los ultimos anos por su comportamiento como un padecimiento venereo de elevada prevalencia que incluso ha alcanzado niveles epidemicos en distintas regiones geogragicas en donde se ha procurado su deteccion de manera intencionada.Se pormenorizan sus peculiares caracteristicas microbiologicas que lo distinguen del resto de los microorganismos y se describen los principales cuadros clinicos que a nivel genital origina y su ingerencia en la esterilidad, en los padecimientos malignos y en las infecciones neonatales; se especifican los mas importantes principios y metodos para su deteccion, asi como los mas efectivos esquemas para su erradicacion; finalmente, se senalan las principales medidas preventivas para evitar su transmision tanto horizontal como vertical