RESUMO
The silencing of p16INK4a (p16) tumor suppressor gene by aberrant methylation has been described as a common event in human tumoral types, such as squamous cell lung carcinoma (SCLC). This is a very frequent histological type in Chile, usually associated to chronic smokers. The purpose of this study was to investigate the p16 methylation status in patients with SCLC. Using a MSP (methylation-specific PCR), we determined that 25 out of 29 (86 percent) of SCLC patients had hypermethylation of p16 gene. There was also a significant concordance between hypermethylation of p16 gene and the presence of SCLC (p = 0.00005). According to our results, smokers have a 202-fold higher risk to develop SCLC than no-smokers (odds ratio, 201,7; 95 percent confidence interval, 7.18-5593). Therefore, our results suggest that hypermethylation of p16 gene is an useful prognostic marker in chronic smokers candidates to develop SCLC and patient with clinic suspicion of SCLC.
El silenciamiento génico por metilación aberrante del gen p16INK4a (p16) ha sido descrito en varios tipos de tumores humanos, como por ejemplo, el carcinoma escamoso de pulmón (CEP), un tipo histológico frecuente en Chile, asociado a tabaquismo crónico. El propósito de este estudio fue determinar el estado de metilación de la región promotora de p16 en CEP. Utilizando un método de PCR específico para metilación, se determinó que un total de 29 pacientes con CEP, 25 (86 por ciento) tenían el gen p16 hipermetilado. Además, se encontró una correlación significativa entre la hipermetilación del gen p16 y la presencia de CEP (p = 0,00005). Por otra parte, estimamos que los individuos fumadores tienen 201,7 veces más riesgo que los no fumadores de desarrollar CEP (odds ratio, 201,7; intervalo de confianza 95 por ciento, 7,18-5.593). Nuestros resultados sugieren que la detección de hipermetilación del gen p16 podría ser utilizada como un marcador molecular de diagnóstico precoz en individuos fumadores con riesgo a desarrollar CEP y en pacientes con sospecha clínica de CEP.
Assuntos
Humanos , Masculino , Feminino , Idoso , Carcinoma de Células Escamosas/genética , Metilação de DNA , Neoplasias Pulmonares/genética , Interpretação Estatística de Dados , Chile/epidemiologia , Estudos Retrospectivos , Marcadores Genéticos , Reação em Cadeia da Polimerase , Risco , Tabagismo/efeitos adversos , Tabagismo/genéticaRESUMO
Internet/world wide web is at the present time, one of the greatest sources of information, knowledge, reference materials, education and training programs for the general population and specially for professionals of clinical laboratories. New equipment, methodologies and products have produced a profound change in the organization and operation of clinical laboratories. This article reviews the importance that these changes have in the efficiency and quality of clinical laboratories
Assuntos
Humanos , Laboratórios/tendências , Desenvolvimento Tecnológico/tendências , Informática Médica/tendências , Publicação Periódica/tendências , Redes de Comunicação de Computadores/tendênciasRESUMO
We measured total serum IgE in 14 patients with allergic diseases and 16 healthy subjects, using 3 commercial ELISA kits. The correlation of results among the 3 kits was analized using Passing and Bablock regression parameters. Results show that measurements of the different kits do not coincide. One kit shows differences using sera from allergic patients. It is concluded that it is not possible to determine exactly the amount of IgE using these kits, specially in subjects with elevated levels
