RESUMO
OBJECTIVES: To prospectively evaluate demographic, anthropometric and health-related quality of life (HRQoL) in pediatric patients with laboratory-confirmed coronavirus disease 2019 (COVID-19). METHODS: This was a longitudinal observational study of surviving pediatric post-COVID-19 patients (n=53) and pediatric subjects without laboratory-confirmed COVID-19 included as controls (n=52) was performed. RESULTS: The median duration between COVID-19 diagnosis (n=53) and follow-up was 4.4 months (0.8-10.7). Twenty-three of 53 (43%) patients reported at least one persistent symptom at the longitudinal follow-up visit and 12/53 (23%) had long COVID-19, with at least one symptom lasting for >12 weeks. The most frequently reported symptoms at the longitudinal follow-up visit were headache (19%), severe recurrent headache (9%), tiredness (9%), dyspnea (8%), and concentration difficulty (4%). At the longitudinal follow-up visit, the frequencies of anemia (11% versus 0%, p=0.030), lymphopenia (42% versus 18%, p=0.020), C-reactive protein level of >30 mg/L (35% versus 0%, p=0.0001), and D-dimer level of >1000 ng/mL (43% versus 6%, p=0.0004) significantly reduced compared with baseline values. Chest X-ray abnormalities (11% versus 2%, p=0.178) and cardiac alterations on echocardiogram (33% versus 22%, p=0.462) were similar at both visits. Comparison of characteristic data between patients with COVID-19 at the longitudinal follow-up visit and controls showed similar age (p=0.962), proportion of male sex (p=0.907), ethnicity (p=0.566), family minimum monthly wage (p=0.664), body mass index (p=0.601), and pediatric pre-existing chronic conditions (p=1.000). The Pediatric Quality of Live Inventory 4.0 scores, median physical score (69 [0-100] versus 81 [34-100], p=0.012), and school score (60 [15-100] versus 70 [15-95], p=0.028) were significantly lower in pediatric patients with COVID-19 at the longitudinal follow-up visit than in controls. CONCLUSIONS: Pediatric patients with COVID-19 showed a longitudinal impact on HRQoL parameters, particularly in physical/school domains, reinforcing the need for a prospective multidisciplinary approach for these patients. These data highlight the importance of closer monitoring of children and adolescents by the clinical team after COVID-19.
Assuntos
COVID-19 , Adolescente , COVID-19/complicações , Teste para COVID-19 , Criança , Humanos , América Latina , Masculino , Estudos Prospectivos , Qualidade de Vida , SARS-CoV-2 , Centros de Atenção Terciária , Síndrome de COVID-19 Pós-AgudaRESUMO
OBJECTIVES: To prospectively evaluate demographic, anthropometric and health-related quality of life (HRQoL) in pediatric patients with laboratory-confirmed coronavirus disease 2019 (COVID-19) METHODS: This was a longitudinal observational study of surviving pediatric post-COVID-19 patients (n=53) and pediatric subjects without laboratory-confirmed COVID-19 included as controls (n=52) was performed. RESULTS: The median duration between COVID-19 diagnosis (n=53) and follow-up was 4.4 months (0.8-10.7). Twenty-three of 53 (43%) patients reported at least one persistent symptom at the longitudinal follow-up visit and 12/53 (23%) had long COVID-19, with at least one symptom lasting for >12 weeks. The most frequently reported symptoms at the longitudinal follow-up visit were headache (19%), severe recurrent headache (9%), tiredness (9%), dyspnea (8%), and concentration difficulty (4%). At the longitudinal follow-up visit, the frequencies of anemia (11% versus 0%, p=0.030), lymphopenia (42% versus 18%, p=0.020), C-reactive protein level of >30 mg/L (35% versus 0%, p=0.0001), and D-dimer level of >1000 ng/mL (43% versus 6%, p=0.0004) significantly reduced compared with baseline values. Chest X-ray abnormalities (11% versus 2%, p=0.178) and cardiac alterations on echocardiogram (33% versus 22%, p=0.462) were similar at both visits. Comparison of characteristic data between patients with COVID-19 at the longitudinal follow-up visit and controls showed similar age (p=0.962), proportion of male sex (p=0.907), ethnicity (p=0.566), family minimum monthly wage (p=0.664), body mass index (p=0.601), and pediatric pre-existing chronic conditions (p=1.000). The Pediatric Quality of Live Inventory 4.0 scores, median physical score (69 [0-100] versus 81 [34-100], p=0.012), and school score (60 [15-100] versus 70 [15-95], p=0.028) were significantly lower in pediatric patients with COVID-19 at the longitudinal follow-up visit than in controls. CONCLUSIONS: Pediatric patients with COVID-19 showed a longitudinal impact on HRQoL parameters, particularly in physical/school domains, reinforcing the need for a prospective multidisciplinary approach for these patients. These data highlight the importance of closer monitoring of children and adolescents by the clinical team after COVID-19.
Assuntos
Humanos , Masculino , Criança , Adolescente , COVID-19/complicações , Qualidade de Vida , Estudos Prospectivos , Centros de Atenção Terciária , Teste para COVID-19 , SARS-CoV-2 , América LatinaRESUMO
OBJECTIVE: The aim of this study was to investigate how maternal polyunsaturated fatty acid intake at different periods during pregnancy affects the composition of polyunsaturated fatty acids in mature human milk. METHODS: A prospective study was conducted involving 45 pregnant women, aged between 18 and 35 y, who had full-term pregnancies and practiced exclusive or predominant breast-feeding. Mature breast milk samples were collected after the 5th postpartum week by manual expression; fatty acid composition was determined by gas chromatography. Fatty acid intake during pregnancy and puerperium was estimated through multiple 24-h dietary recalls. Linear regression models, adjusted by postpartum body mass index and deattenuated, were used to determine associations between estimated fatty acids in maternal diet during each trimester of pregnancy and fatty acid content in mature human milk. RESULTS: A positive association was identified between maternal intake of eicosapentaenoic acid (ß, 1.873; 95% confidence interval [CI], 0.545, 3.203) and docosahexaenoic acid (ß, 0.464; 95% CI, 0.212-0.714) during the third trimester of pregnancy, as well as the maternal dietary ω-3 to ω-6 ratio (ß, 0.093; 95% CI, 0.016-0.170) during the second and third trimesters and postpartum period, with these fatty acids content in mature breast milk. CONCLUSIONS: The maternal dietary docosahexaenoic acid and eicosapentaenoic acid content during late pregnancy may affect the fatty acid composition of mature breast milk. Additionally, the maternal dietary intake of ω-3 to ω-6 fatty acid ratio, during late pregnancy and the postpartum period, can affect the polyunsaturated fatty acid composition of breast milk.
Assuntos
Ácidos Graxos Ômega-3/administração & dosagem , Ácidos Graxos Ômega-6/administração & dosagem , Comportamento Alimentar , Leite Humano/química , Terceiro Trimestre da Gravidez , Adolescente , Adulto , Cromatografia Gasosa , Dieta , Gorduras na Dieta/administração & dosagem , Feminino , Humanos , Modelos Lineares , Fenômenos Fisiológicos da Nutrição Materna , Período Pós-Parto , Gravidez , Estudos Prospectivos , Fatores Socioeconômicos , Adulto JovemRESUMO
Dietary choline is required for proper structure and dynamics of cell membranes, lipoprotein synthesis, and methyl-group metabolism. In mammals, choline is synthesized via phosphatidylethanolamine N-methyltransferase (Pemt), which converts phosphatidylethanolamine to phosphatidylcholine. Pemt(-/-) mice have impaired VLDL secretion and developed fatty liver when fed a high-fat (HF) diet. Because of the reduction in plasma lipids, Pemt(-/-)/low-density lipoprotein receptor knockout (Ldlr(-/-)) mice are protected from atherosclerosis. The goal of this study was to investigate the importance of dietary choline in the metabolic phenotype of Pemt(-/-)/Ldlr(-/-) male mice. At 10-12 wk of age, Pemt(+/+)/Ldlr(-/-) (HF(+/+)) and half of the Pemt(-/-)/Ldlr(-/-) (HF(-/-)) mice were fed an HF diet with normal (1.3 g/kg) choline. The remaining Pemt(-/-)/Ldlr(-/-) mice were fed an HF diet supplemented (5 g/kg) with choline (HFCS(-/-) mice). The HF diet contained 60% of calories from fat and 1% cholesterol, and the mice were fed for 16 d. HF(-/-) mice lost weight and developed hepatomegaly, steatohepatitis, and liver damage. Hepatic concentrations of free cholesterol, cholesterol-esters, and triglyceride (TG) were elevated by 30%, 1.1-fold and 3.1-fold, respectively, in HF(-/-) compared with HF(+/+) mice. Choline supplementation normalized hepatic cholesterol, but not TG, and dramatically improved liver function. The expression of genes involved in cholesterol transport and esterification increased by 50% to 5.6-fold in HF(-/-) mice when compared with HF(+/+) mice. Markers of macrophages, oxidative stress, and fibrosis were elevated in the HF(-/-) mice. Choline supplementation normalized the expression of these genes. In conclusion, HF(-/-) mice develop liver failure associated with altered cholesterol metabolism when fed an HF/normal choline diet. Choline supplementation normalized cholesterol metabolism, which was sufficient to prevent nonalcoholic steatohepatitis development and improve liver function. Our data suggest that choline can promote liver health by maintaining cholesterol homeostasis.
Assuntos
Colesterol/metabolismo , Colina/administração & dosagem , Dieta Hiperlipídica/efeitos adversos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Animais , Ésteres do Colesterol/metabolismo , Fígado Gorduroso/tratamento farmacológico , Fígado Gorduroso/etiologia , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/patologia , Masculino , Camundongos , Camundongos Knockout , Hepatopatia Gordurosa não Alcoólica , Fosfatidiletanolamina N-Metiltransferase/sangue , Receptores de LDL/sangue , Triglicerídeos/metabolismoRESUMO
OBJETIVOS: Avaliar a composição de ácidos graxos do leite humano maduro de mulheres residentes em área distante da costa litorânea brasileira. MÉTODOS: Amostras de leite materno maduro foram obtidas de 47 mulheres lactantes com idade entre 18 e 35 anos, que tiveram partos a termo e em aleitamento exclusivo ou predominante. A coleta de leite se deu a partir da 5ª semana pós-parto, por meio de ordenha manual. A composição de ácidos graxos do leite foi determinada por cromatografia gasosa. RESULTADOS: Verificou-se que a concentração de eicosapentaenoico (0,08%) foi superior ao observado em estudos brasileiros prévios. Entretanto, o teor de docosahexaenoico (0,09%) encontrado no leite humano foi um dos menores já verificados no mundo. O teor de ácidos graxos trans (2,05%) foi similar ao relatado em estudos nacionais prévios à obrigatoriedade de declaração do teor deste em rótulos de alimentos, sugerindo que esta medida não surtiu efeito na redução de seu teor na dieta habitual das mulheres. CONCLUSÕES: Baixo teor de docosahexaenoico e elevada concentração de ácidos graxos trans foram verificados no leite materno maduro de mulheres residentes em área distante da costa litorânea brasileira.
OBJECTIVES: To evaluate the fatty acid composition of mature human milk of women living far from the coastal area of Brazil. METHODS: Mature breast milk samples were obtained from 47 lactating women aged between 18 and 35 years, who delivered their babies at term and who exclusively or predominantly breastfed. Milk collection took place after the fifth week postpartum by hand expression. The fatty acid composition of the milk was determined by gas chromatography. RESULTS: It was observed that the concentration of eicosapentaenoic acid (0.08%) was higher than that observed in previous studies in Brazil. However, the content of docosahexaenoic acid (0.09%) found in human milk was one of the lowest verified in the world. The content of trans fatty acids (2.05%) was similar to that reported in national studies previous to the mandatory declaration of this fatty acid content in food labels, suggesting that this measure had no effect on reducing the content of this fatty acid in the usual diet of women. CONCLUSIONS: Low levels of docosahexaenoic acid and high concentrations of trans fatty acids were observed in mature breast milk of women living far from the coastal area in Brazil.
Assuntos
Adolescente , Adulto , Feminino , Humanos , Adulto Jovem , Ácidos Graxos/análise , Leite Humano/química , Brasil , Gorduras na Dieta/análise , Ácidos Docosa-Hexaenoicos/análise , Ácido Eicosapentaenoico/análise , Lactação/fisiologia , Estudos Prospectivos , Ácidos Graxos trans/análise , População Urbana/estatística & dados numéricosRESUMO
OBJECTIVES: To evaluate the fatty acid composition of mature human milk of women living far from the coastal area of Brazil. METHODS: Mature breast milk samples were obtained from 47 lactating women aged between 18 and 35 years, who delivered their babies at term and who exclusively or predominantly breastfed. Milk collection took place after the fifth week postpartum by hand expression. The fatty acid composition of the milk was determined by gas chromatography. RESULTS: It was observed that the concentration of eicosapentaenoic acid (0.08%) was higher than that observed in previous studies in Brazil. However, the content of docosahexaenoic acid (0.09%) found in human milk was one of the lowest verified in the world. The content of trans fatty acids (2.05%) was similar to that reported in national studies previous to the mandatory declaration of this fatty acid content in food labels, suggesting that this measure had no effect on reducing the content of this fatty acid in the usual diet of women. CONCLUSIONS: Low levels of docosahexaenoic acid and high concentrations of trans fatty acids were observed in mature breast milk of women living far from the coastal area in Brazil.
Assuntos
Ácidos Graxos/análise , Leite Humano/química , Adolescente , Adulto , Brasil , Gorduras na Dieta/análise , Ácidos Docosa-Hexaenoicos/análise , Ácido Eicosapentaenoico/análise , Feminino , Humanos , Lactação/fisiologia , Estudos Prospectivos , Ácidos Graxos trans/análise , População Urbana/estatística & dados numéricos , Adulto JovemRESUMO
PURPOSE: The increase in fructose consumption is paralleled by a higher incidence of obesity worldwide. This monosaccharide is linked to metabolic syndrome, being associated with hypertriglyceridemia, hypertension, insulin resistance and diabetes mellitus. It is metabolized principally in the liver, where it can be converted into fatty acids, which are stored in the form of triglycerides leading to NAFLD. Several models of NAFLD use diets high in simple carbohydrates. Thus, this study aimed to describe the major metabolic changes caused by excessive consumption of fructose in humans and animals and to present liver abnormalities resulting from high intakes of fructose in different periods of consumption and experimental designs in Wistar rats. METHODS: Two groups of rats were fasted for 48 hours and refed for 24 or 48 hours with a diet containing 63% fructose. Another group of rats was fed an diet with 63% fructose for 90 days. RESULTS: Refeeding for 24 hours caused accumulation of large amounts of fat, compromising 100% of the hepatocytes. The amount of liver fat in animals refed for 48 hours decreased, remaining mostly in zone 2 (medium-zonal). In liver plates of Wistar rats fed 63% fructose for 45, 60 and 90 days it's possible to see that there is an increase in hepatocytes with fat accumulation according to the increased time; hepatic steatosis, however, is mild, compromising about 20% of the hepatocytes. CONCLUSIONS: Fructose is highly lipogenic, however the induction of chronic models in NAFLD requires long periods of treatment. The acute supply for 24 or 48 hours, fasted rats can cause big changes, liver steatosis with macrovesicular in all lobular zones.
Assuntos
Modelos Animais de Doenças , Fígado Gorduroso/metabolismo , Frutose/metabolismo , Fígado/metabolismo , Síndrome Metabólica/metabolismo , Edulcorantes/metabolismo , Animais , Fígado Gorduroso/etiologia , Frutose/efeitos adversos , Masculino , Síndrome Metabólica/etiologia , Hepatopatia Gordurosa não Alcoólica , Obesidade/etiologia , Obesidade/metabolismo , Ratos , Ratos Wistar , Edulcorantes/efeitos adversos , Fatores de TempoRESUMO
PURPOSE: The increase in fructose consumption is paralleled by a higher incidence of obesity worldwide. This monosaccharide is linked to metabolic syndrome, being associated with hypertriglyceridemia, hypertension, insulin resistance and diabetes mellitus. It is metabolized principally in the liver, where it can be converted into fatty acids, which are stored in the form of triglycerides leading to NAFLD. Several models of NAFLD use diets high in simple carbohydrates. Thus, this study aimed to describe the major metabolic changes caused by excessive consumption of fructose in humans and animals and to present liver abnormalities resulting from high intakes of fructose in different periods of consumption and experimental designs in Wistar rats. METHODS: Two groups of rats were fasted for 48 hours and reefed for 24 or 48 hours with a diet containing 63 percent fructose. Another group of rats was fed an diet with 63 percent fructose for 90 days. RESULTS: Refeeding for 24 hours caused accumulation of large amounts of fat, compromising 100 percent of the hepatocytes. The amount of liver fat in animals refed for 48 hours decreased, remaining mostly in zone 2 (medium-zonal). In liver plates of Wistar rats fed 63 percent fructose for 45, 60 and 90 days it's possible to see that there is an increase in hepatocytes with fat accumulation according to the increased time; hepatic steatosis, however, is mild, compromising about 20 percent of the hepatocytes. CONCLUSIONS: Fructose is highly lipogenic, however the induction of chronic models in NAFLD requires long periods of treatment. The acute supply for 24 or 48 hours, fasted rats can cause big changes, liver steatosis with macrovesicular in all lobular zones.
OBJETIVO: O aumento do consumo de frutose é concomitante a maior incidência mundial de obesidade. Este monossacarídeo está relacionado à Síndrome Metabólica, sendo vinculado à hipertrigliceridemia, hipertensão arterial, resistência à insulina e diabetes mellitus. É metabolizada principalmente no fígado, onde pode ser convertida em ácidos graxos, os quais serão estocados na forma de trigligérides ocasionando a esteatose hepática não alcoólica (NAFLD). Vários modelos de NAFLD utilizam dietas ricas em carboidratos simples. Desta forma, este trabalho teve como objetivos descrever as principais alterações metabólicas causadas pelo consumo excessivo de frutose em humanos e em animais e apresentar as alterações hepáticas decorrentes da alta ingestão de frutose em diferentes períodos de consumo e desenhos experimentais em ratos Wistar. MÉTODOS: Dois grupos de ratos Wistar foram mantidos em jejum durante 48 horas e realimentados por 24 ou 48 horas com dieta contendo 63 por cento de frutose. Outro grupo de ratos Wistar foi alimentado com 63 por cento de frutose durante 90 dias. RESULTADOS: A realimentação por 24 horas provocou acúmulo de grande quantidade de gordura. A quantidade de gordura hepática nos animais realimentados por 48 horas diminuiu, mantendo-se principalmente nas zona 2 (medio-zonal). Em fígados de ratos Wistar alimentados com 63 por cento de frutose até 90 dias foi possível observar que há aumento de hepatócitos com acúmulo de gordura consequente ao aumento do tempo, no entanto a esteatose hepática é leve (20 por cento). CONCLUSÕES: A frutose é altamente lipogênica, no entanto a indução de NAFLD em modelos crônicos necessita de longos períodos de tratamento. A oferta aguda, por 24 ou 48 horas, a ratos mantidos em jejum é capaz de ocasionar grandes mudanças hepáticas, com presença de esteatose macrovesicular em todas as zonas lobulares.
Assuntos
Animais , Masculino , Ratos , Modelos Animais de Doenças , Fígado Gorduroso/metabolismo , Frutose/metabolismo , Fígado/metabolismo , Síndrome Metabólica/metabolismo , Edulcorantes/metabolismo , Fígado Gorduroso/etiologia , Frutose/efeitos adversos , Síndrome Metabólica/etiologia , Obesidade/etiologia , Obesidade/metabolismo , Ratos Wistar , Edulcorantes/efeitos adversos , Fatores de TempoRESUMO
Modelo de estudo: Estudo experimental. Objetivos: este estudo teve como objetivo avaliar e caracterizar a dieta hipoprotéica como um modelo experimental para estudo de EHNA. Métodos: foram utilizados ratos da linhagem Wistar divididos em dois grupos com dietas isocalóricas: controle (GC) no qual a dieta utilizada seguiu o preconizado pela AIN-93 e hipoprotéico (GH) com quantidade de proteína reduzida de 20% para 10%. As dietas e água foram ofertadas ad libitum por quatro semanas. Após esse período, os animais foram sacrificados e analisados: glicemia; nitrogênio urinário; proteína sérica; gordura hepática; colesterol; variação de peso e quantidade de ração consumida. Resultados: glicemia e nitrogênio urinário não apresentaram diferenças significativas entre GC e GH (p>0,05), a variação de peso no último dia do experimento foi significativa (p<0,02). A porcentagem de gordura hepática foi estatisticamente maior no GH, quando comparado ao GC (p<0,04). Foram menores o nível de colesterol (p<0,01) e proteína sérica (p<0,005) no GH. A quantidade de dieta consumida não foi diferente entre os grupos, considerando-se as médias de ingestão semanal. Conclusões: neste trabalho a dieta hipoprotéica constitui um modelo de indução de EHNA que pode ser caracterizada pela diminuição da proteína sérica e do colesterol plasmático e aumento da gordura hepática, entretanto não ocorreram alterações na glicemia sugerindo que não existiu mudança na sensibilidade à insulina, constituindo assim um modelo falho para estudar um dos principais fatores de risco para o estabelecimento da EHNA, a resistência à insulina.
Model of study: Experimental study. Objectives: this study had as objective evaluate and characterize a low protein diet as an experimental model for non alcoholic fatty liver disease(NAFLD). Methods: male Wistar rats were divided in two groups with isocaloric diets: control (GC) in which the used diet followed praised for the AIN-93 and the low protein (GH), with reduced amount of protein of 20% for 10%. The diets and water had been offered ad libitum over four weeks. After this period the animals were sacrificed and analyzed: glycemia; urinary nitrogen; serum protein; liver total lipids; cholesterol; weight variation and food consumed. Results: the glycemia and the urinary nitrogen had not presented significant differences between GC and GH (p>0,05). The change of weight in the last day of the experiment was significant (p<0,02). The percentage of total liver lipids was higher in the GH, when compared with the GC (p<0,04). There was lower levels of cholesterol (p<0,01) and serum protein (0,005) in GH. The food consumed was not different between the groups. Conclusions: in this paper the low protein diet constitutes a model of NAFLD induction that can be characterized for serum protein and plasmatic cholesterol and increased fat in the liver, however not alterations in the glycemia suggest no changes in insulin sensitivity, thus constituting a defective model to study one of the main factors of risk for the establishment of the NAFLD, the resistance to insulin.