RESUMO
OBJECTIVE: To investigate the effect of antisense MMP-2 by transfecting the Ad-aMMP-2 on the vascular endothelial cells (VEC) derived from human proferating hemangiomas in vitro. METHODS: Three groups. Group M199, Group Ad-GFP and Group Ad-aMMP-2 were tested in this study with the 100 multiplicity of infection (MOI). Reverse transcription PCR (RT-PCR), Western Blotting and Gelatin Zymography analyses were applied to evaluate the level of endogenous MMP-2 expression. RESULTS: The decreased expression level of endogenous MMP-2 mRNA and protein excretion of MMP-2 and Gelatin Zymography analyses of liveness of Group Ad-aMMP-2 were observed by comparison with Group M199 and Group Ad-GFP (P<0.05). CONCLUSION: Ad-aMMP-2 could inhibit the secretion of MMP-2 from VEC in vitro.
