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1.
Morphologie ; 108(361): 100760, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38157748

RESUMO

BACKGROUND: Myocardial bridge (MB) is described as an abnormal band of myocardium covering a variable portion of any coronary artery. METHODS: The current study explores the presence of MB throughout the coronary arterial system and provides a morphometric description through instrumented dissection of a sample of 100 human hearts. The study shows a higher prevalence of MB in the Mexican population than in previous reports. RESULTS: In the total sample (n=100), MB was identified in 96% of it. A total of 421 MBs were observed, with a mean of 4.38mm (±0.28) per dissected heart. The most frequently affected vessel is the anterior interventricular artery where a total of 52 MBs were found, of the total sample studied. DISCUSSION: The high prevalence of MB among Mexican patients could be the result of a genetic association for this population or the neoformation of MB after birth due to lifestyle-associated factors. Further studies are required to better understand the high prevalence of MB among Mexican subjects.


Assuntos
Ponte Miocárdica , Humanos , México/epidemiologia , Masculino , Feminino , Prevalência , Ponte Miocárdica/epidemiologia , Ponte Miocárdica/patologia , Pessoa de Meia-Idade , Adulto , Idoso , Vasos Coronários/anatomia & histologia , Idoso de 80 Anos ou mais , Miocárdio/patologia , Adulto Jovem
2.
Rev Sci Instrum ; 89(7): 073303, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30068099

RESUMO

In this work, two compact, permanent magnet, electron spectrometers have been built to measure the electron beam energy at the Dual Axis Radiographic Hydrodynamic Test facility. Using H- and OH- anions, the spectrometers were calibrated at the Special Technologies Laboratory in Santa Barbara, California (USA). The spectrometers were mounted on a custom drift tube that allows the magnet assemblies to be translated, which increases the path length of the electrons traveling through the magnetic field and therefore increases the upper bound of the measurable electron kinetic energy. The measurable range of electron kinetic energies is between 2.8 MeV-4.1 MeV for the first spectrometer and 14.1 MeV-21.1 MeV for the second spectrometer, with an overall measurement uncertainty of 0.32%.

3.
Chemosphere ; 86(10): 985-93, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22197016

RESUMO

Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic substances which are resistant to environmental degradation due to their highly hydrophobic nature. Soils contaminated with PAHs pose potential risks to human and ecological health, therefore concern over their adverse effects have resulted in extensive studies on their removal from contaminated soils. The main purpose of this study was to compare experimental results of PAHs removal, from a natural certified soil polluted with PAHs, by biological methods (using bioaugmentation and biostimulation in a solid-state culture) with those from supercritical fluid extraction (SFE), using supercritical ethane as solvent. The comparison of results between the two methods showed that maximal removal of naphthalene, acenaphthene, fluorene, and chrysene was performed using bioremediation; however, for the rest of the PAHs considered (fluoranthene, pyrene, and benz(a)anthracene) SFE resulted more efficient. Although bioremediation achieved higher removal ratios for certain hydrocarbons and takes advantage of the increased rate of natural biological processes, it takes longer time (i.e. 36 d vs. half an hour) than SFE and it is best for 2-3 PAHs rings.


Assuntos
Cromatografia com Fluido Supercrítico , Recuperação e Remediação Ambiental/métodos , Hidrocarbonetos Policíclicos Aromáticos/análise , Poluentes do Solo/análise , Biodegradação Ambiental , Hidrocarbonetos Policíclicos Aromáticos/química , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Solo , Poluentes do Solo/química , Poluentes do Solo/metabolismo
4.
Parasitol Res ; 101(6): 1627-35, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17726617

RESUMO

To investigate the importance of serine proteases in Leishmania amazonensis promastigotes, we analyzed the effects of classical serine protease inhibitors and a Kunitz-type inhibitor, obtained from sea anemone Stichodactyla helianthus (ShPI-I), on the viability and morphology of parasites in culture. Classical inhibitors were selected on the basis of their ability to inhibit L. amazonensis serine proteases, previously described. The N-tosyl-L: -phenylalanine chloromethyl ketone (TPCK) and benzamidine (Bza) inhibitors, which are potential Leishmania proteases inhibitors, in all experimental conditions reduced the parasite viability, with regard to time dependence. On the other hand, N-tosyl-lysine chloromethyl ketone (TLCK) did not significantly affect the parasite viability, as it was poor Leishmania enzymes inhibitor. Ultrastructural analysis demonstrated that both Bza and TPCK induced changes in the flagellar pocket region with membrane alteration, including bleb formation. However, TPCK effects were more pronounced than those of Bza in Leishmania flagellar pocket in plasma membrane, and intracellular vesicular bodies was visualized. ShPI-I proved to be a powerful inhibitor of L. amazonensis serine proteases and the parasite viability. The ultrastructural alterations caused by ShPI-I were more dramatic than those induced by the classical inhibitors. Vesiculation of the flagellar pocket membrane, the appearance of a cytoplasmic vesicle that resembles an autophagic vacuole, and alterations of promastigotes shape resulted.


Assuntos
Leishmania mexicana/efeitos dos fármacos , Leishmania mexicana/crescimento & desenvolvimento , Serina Endopeptidases/metabolismo , Inibidores de Serina Proteinase/farmacologia , Animais , Colorimetria , Leishmania mexicana/enzimologia , Leishmania mexicana/ultraestrutura , Microscopia Eletrônica , Testes de Sensibilidade Parasitária , Sais de Tetrazólio , Tiazóis
5.
Toxicon ; 43(2): 219-23, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15019482

RESUMO

An inhibitor active against pancreatic trypsin was found in the crude extract from the sea hares Aplysia dactylomelaRang, 1828. A stronger inhibitory activity against human plasma kallikrein was detectable after treating this extract at 60 degrees C, for 30 min. The plasma kallikrein inhibitor (AdKI) purification was achieved by acetone fractionation (80%) v/v, ion-exchange chromatography on Mono Q column and gel filtration chromatography on Superdex 75 column (FPLC system). By the latter a molecular mass of 2900 Da was estimated. The purified inhibitor strongly inhibits human plasma kallikrein with a K(i) value of 2.2 x 10(-10)M, while human plasmin and pancreatic trypsin were inhibited with K(i) values of 1.8 x 10(-9) and 4.7 x 10(-9)M, respectively. Chymotrypsin, pancreatic elastase, pancreatic kallikrein and thrombin are not inhibited. The effect of AdKI on plasma kallikrein was confirmed by the prolongation of activated partial thromboplastin time, using a clotting time assay. The inhibitor did not affect prothrombin time or thrombin time. AdKi is a more specific inhibitor than other serine proteinase inhibitors from marine invertebrates.


Assuntos
Aplysia/química , Calicreína Plasmática/antagonistas & inibidores , Animais , Coagulação Sanguínea/efeitos dos fármacos , Fracionamento Químico , Cromatografia em Gel , Humanos , Fatores de Tempo
6.
Cienc. ginecol ; 6(1): 1-6, ene. 2002. tab
Artigo em Es | IBECS | ID: ibc-14242

RESUMO

La endometriosis se define como la presencia de tejido endometrial ectópico fuera de la cavidad uterina normal. El tratamiento médico convencional tiene como objetivo eliminar o disminuir los depósitos de tejido endometrial ectópico. Hoy en día todavía no está clara la mejor estrategia terapéutica para esta patología. La elección del tratamiento está condicionada por múltiples factores (edad, fertilidad, gravedad del proceso,...) En este artículo se revisan las diferentes posibilidades de tratamiento médico de la endometriosis (AU)


Assuntos
Feminino , Humanos , Endometriose/tratamento farmacológico
7.
Rev. iberoam. trombos. hemost. (Ed. impr.) ; 13(3): 110-112, sept. 2000. graf
Artigo em ES | IBECS | ID: ibc-11762

RESUMO

Hay evidencia de la presencia de alteraciones en la coagulación que pueden agravar el estado de preeclampsia. Por una parte se ha involucrado a las células mononucleares y por tanto a la generación de factor tisular y por otra a la resistencia a la proteína C como mecanismos en la etiopatogenia de problemas trombóticos. El objetivo del presente trabajo fue evaluar estos factores en una muestra de 20 mujeres embarazadas con preeclampsia y 20 mujeres embarazadas sanas. Estadísticamente no se encontraron diferencias con relacion a uno y otro grupo, con la salvedad de 2 mujeres del grupo con preeclampsia que mostraron resistencia a la proteína C. (AU)


Assuntos
Adulto , Gravidez , Feminino , Humanos , Pré-Eclâmpsia/sangue , Leucócitos Mononucleares/metabolismo , Proteína C/farmacologia , Coagulação Sanguínea/fisiologia , Fator VIII/biossíntese , Estudos de Casos e Controles , Testes de Coagulação Sanguínea
8.
Prep Biochem Biotechnol ; 29(1): 23-33, 1999 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10069430

RESUMO

In this work, we report the isolation of a factor from the culture supernatant of confluent fibroblasts from human cervix with the diagnosis of uterine myomatosis. This factor possesses the capacity to inhibit the proliferation of normal fibroblasts. The proliferation inhibitor factor (PIF) was purified from the culture supernatant by precipitation with 80% ammonium sulfate, and by molecular sieve chromatography. Our results indicate that PIF is a protein of 23 kDa, which is highly sensitive to trypsin treatment, and is thermolabile, since temperatures equal to, or above, 60 degrees C eliminate the protein activity in 15 to 20 min. Western blot analyses identified no cross reactions of the purified PIF with TGF-alpha, TNFalpha, IFNgamma, or IL-1beta, suggesting that PIF is a new protein belonging to the group of factors secreted by fibroblasts able to inhibit cellular proliferation.


Assuntos
Inibidores do Crescimento/isolamento & purificação , Leiomioma/química , Proteínas de Neoplasias/isolamento & purificação , Neoplasias Uterinas/química , Western Blotting , Contagem de Células , Cromatografia em Gel , Relação Dose-Resposta a Droga , Fator de Crescimento Epidérmico/farmacologia , Feminino , Fibroblastos/química , Violeta Genciana/análise , Humanos , Interferon gama/farmacologia , Interleucina-1/farmacologia , Fator de Crescimento Transformador beta/farmacologia , Células Tumorais Cultivadas , Fator de Necrose Tumoral alfa/farmacologia
9.
Vet Pathol ; 35(6): 547-9, 1998 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9823599

RESUMO

A review of case materials at the Armed Forces Institute of Pathology identified six cases of prostatitis in domestic ferrets (Mustela putorius furo). Five of these ferrets (83%) had one or more cysts and the remaining ferret had a paraprostatic cyst. Three of the six ferrets (50%) exhibited various degrees of prostatic squamous metaplasia. Inflammation ranged from subacute to chronic-active or pyogranulomatous. In addition to the prostatic lesions, hyperplastic and/or neoplastic adrenocortical lesions were present in 4/6 (66%) ferrets; 1/6 (17%) ferrets had previously been clinically diagnosed with adrenal gland-associated endocrinopathy. The remaining ferret had previously had the right adrenal gland removed, but the reason for the removal is unknown. Based upon the histologic findings in these six ferrets, there appears to be an association between proliferative adrenal lesions and cystic prostatitis in domestic ferrets.


Assuntos
Neoplasias do Córtex Suprarrenal/veterinária , Cistos/veterinária , Furões , Próstata/patologia , Prostatite/veterinária , Neoplasias do Córtex Suprarrenal/complicações , Neoplasias do Córtex Suprarrenal/patologia , Animais , Cistos/complicações , Cistos/patologia , Hiperplasia/complicações , Hiperplasia/patologia , Hiperplasia/veterinária , Masculino , Prostatite/complicações , Prostatite/patologia
10.
J Prosthet Dent ; 72(4): 351-4, 1994 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-7990038

RESUMO

The retention of amalgam and gallium alloy restorations in proximal box forms was measured in vitro, and three different adhesives to conventional undercuts were compared. For control, restorations were placed without undercuts or adhesives. No significant difference was found between amalgam and gallium alloys with each of the five methods of retention used. Alloys placed without retention or adhesives were significantly less retentive than all other groups. When Tytin alloy was used, no difference was found in retention among the restorations retained with Panavia or All-Bond adhesive or an occlusal dovetail and retention grooves, but Amalgambond adhesive was less retentive than all three of these methods. When gallium alloy was used, both Panavia and All-Bond adhesive were more retentive than undercuts, but the effect of Amalgambond adhesive was more retentive than undercuts, but the effect of Amalgambond adhesive was comparable to that of undercuts. The results of this study indicate that adhesives could be used in place of traditional undercuts to retain amalgam and gallium alloys, thus saving a considerable amount of tooth structure.


Assuntos
Adesivos/química , Ligas Dentárias/química , Amálgama Dentário/química , Colagem Dentária , Restauração Dentária Permanente , Gálio/química , Cimentos de Resina , Resinas Sintéticas/química , Condicionamento Ácido do Dente , Resinas Compostas/química , Preparo da Cavidade Dentária/classificação , Preparo da Cavidade Dentária/métodos , Restauração Dentária Permanente/classificação , Restauração Dentária Permanente/métodos , Adesivos Dentinários/química , Humanos , Metacrilatos/química , Fosfatos/química , Estresse Mecânico , Propriedades de Superfície
11.
Eur J Biochem ; 212(3): 675-84, 1993 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-8462542

RESUMO

The solution structure of a 55-amino-acid Kunitz-type proteinase inhibitor, ShPI, purified from the Caribbean sea anemone Stichodactyla helianthus, was determined by NMR spectroscopy. Nearly complete sequence-specific 1H-NMR assignments were obtained at pH 4.6 and 36 degrees C, and stereo-specific assignments were determined for 23 pairs of diastereotopic substituents. A data set of 666 upper distance limit constraints and 122 dihedral angle constraints collected on this basis was used as input for a structure calculation with the program DIANA. Following energy minimization with the program OPAL, the average root-mean-square diviation (RMSD) of the 20 DIANA conformers used to represent the solution structure relative to the mean structure is 61 pm for all backbone atoms N, C alpha and C', and 106 pm for all heavy atoms of residues 2-53. This high-quality solution structure of ShPI has a nearly identical molecular architecture as the bovine pancreatic trypsin inhibitor (BPTI), despite a mere 35% of sequence similarity between the two proteins. Exchange rates measured for 48 out of the 51 backbone amide protons showed that the positions of 20 slowly exchanging amide protons correlate well with hydrogen bonds involving these protons in the energy-minimized solution structure. The solution structure of ShPI is compared to the four homologous proteins for which the three-dimensional structure is also available.


Assuntos
Estrutura Secundária de Proteína , Anêmonas-do-Mar , Inibidor da Tripsina de Soja de Kunitz/química , Sequência de Aminoácidos , Animais , Espectroscopia de Ressonância Magnética/métodos , Modelos Moleculares , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , Software , Soluções , Inibidor da Tripsina de Soja de Kunitz/isolamento & purificação
12.
Ginecol Obstet Mex ; 59: 246-8, 1991 Aug.
Artigo em Espanhol | MEDLINE | ID: mdl-1765305

RESUMO

Ten patients with isthmico-cervical leiomyoma as the only one localization, were surgically treated, from Jan, 1989 to May, 1990. It was 2.8% of all treated myomatosis. Average age was 42.4 years, with gestation of 1.1 per patients. Nine cases were treated by the abdominal via. One case presented with a recurrence. These cases represent a technico-surgical difficulty, due to tumor localization and size, as well as an ample knowledge about pelvic structures, in order to avoid digestive tract lesions, urinary and vascular. The present series confirms that this type of surgery is justified considering morbi-mortality, so it would be possible a definite histopathological evaluation.


Assuntos
Leiomioma , Neoplasias do Colo do Útero , Adulto , Feminino , Humanos , Histerectomia , Leiomioma/epidemiologia , Leiomioma/patologia , Leiomioma/cirurgia , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Prevalência , Neoplasias do Colo do Útero/epidemiologia , Neoplasias do Colo do Útero/patologia , Neoplasias do Colo do Útero/cirurgia
13.
Diabetologia ; 27(4): 482-3, 1984 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-6439586

RESUMO

Screening for red blood cell sorbitol dehydrogenase deficiency in 12 different mammalian species was performed. A wide inter-species variability in red cell sorbitol dehydrogenase with a virtually complete deficiency in pigs was observed. Aldose reductase and sorbitol dehydrogenase activities in 12 different pig tissues also were measured. Aldose reductase activity was present in all the tissues studied, whereas organ specificity for sorbitol dehydrogenase was observed. Sorbitol dehydrogenase activity was not detectable in lenses, among other tissues, making the pig a potential model for studies in experimental diabetes, particularly for the investigation of sorbitol dehydrogenase deficiency as a risk factor in the development of cataracts.


Assuntos
Diabetes Mellitus Experimental/enzimologia , L-Iditol 2-Desidrogenase/deficiência , Desidrogenase do Álcool de Açúcar/deficiência , Suínos/metabolismo , Aldeído Redutase/metabolismo , Animais , Bovinos , Cricetinae , Cabras , Cavalos , L-Iditol 2-Desidrogenase/metabolismo , Mesocricetus , Camundongos , Camundongos Endogâmicos BALB C , Especificidade de Órgãos , Perissodáctilos , Coelhos , Ratos , Ratos Endogâmicos , Ovinos , Especificidade da Espécie
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