Assuntos
Disgenesia Gonadal 46 XY/diagnóstico , Aneurisma da Aorta Torácica/diagnóstico , Insuficiência da Valva Aórtica/diagnóstico , Cateterismo Cardíaco , Dor no Peito/etiologia , Diagnóstico Diferencial , Cardiopatias Congênitas/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios XAssuntos
Desinfetantes/toxicidade , Escherichia coli/efeitos dos fármacos , Medições Luminescentes , Mutagênicos/toxicidade , Conservantes Farmacêuticos/toxicidade , Aldeídos/química , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/fisiologia , Mononucleotídeo de Flavina/química , Morfolinas/análise , Morfolinas/metabolismo , Morfolinas/toxicidade , Mutagênicos/análise , Mutagênicos/metabolismo , Plasmídeos , Conservantes Farmacêuticos/análise , Conservantes Farmacêuticos/metabolismo , Tiazóis/análise , Tiazóis/metabolismo , Tiazóis/toxicidade , Triazinas/análise , Triazinas/metabolismo , Triazinas/toxicidadeRESUMO
A comparative study of the expression of retroviral envelope antigen by T and B lymphocytes at different maturational stages was made with chickens typed for genomic ev loci. All lymphocyte subclasses from chf(-) chickens were negative for expression of retroviral envelope antigen. By contrast, cells of the lymphocyte lineage from all the chf(+) lines examined were positive for expression, with the levels of envelope antigen in the lymphoblasts exceeding the levels in the immature and resting cells. This pattern of lymphocyte expression was found in lines of chickens in which the expression of chf in fibroblasts was encoded by the ev 3, ev 6, or ev 9 loci. Plasma cells from those chf(+) lines possessing ev 6 expressed higher levels of envelope antigen than the lymphoblasts, an effect not observed with the chf(+) lines lacking ev 6.
Assuntos
Antígenos Virais/genética , Vírus da Leucose Aviária/genética , Genes Virais , Linfócitos/imunologia , Proteínas do Envelope Viral/genética , Animais , Vírus da Leucose Aviária/imunologia , Linfócitos B/imunologia , Galinhas , Fibroblastos/imunologia , Citometria de Fluxo , Imunofluorescência , Genótipo , Humanos , Fenótipo , Linfócitos T/imunologiaRESUMO
Fatty acid synthetase from Saccharomyces cerevisiae is a multifunctional enzyme which catalyzes the synthesis of long chain fatty acids from acetyl- and malonyl-CoA. The enzyme is composed of two nonidentical subunits, alpha (Mr = 212,000) and beta (Mr = 203,000), which are coded for by two unlinked genes FAS2 and FAS1, respectively. Individual yeast strains containing mutations in either of the FAS genes were transformed with a bank of yeast DNA sequences in the vector YEp13. Plasmids YEpFAS1 and YEpFAS2 were selected by their ability to complement the fas1 or fas2 mutations, respectively. Additionally, we utilized an immunologic screening of a second yeast DNA bank and selected two clones 33F1 and 102B5 which produce antigenically reactive material to anti-yeast fatty acid synthetase antibodies. Through Southern hybridization experiments and restriction endonuclease mapping, a region of 5.3 kilobase pairs of 33F1 was shown to be homologous with YEpFAS1, and a span of 3.4 kilobase pairs of 102B5 was homologous with YEpFAS2. These experiments identify the yeast DNA sequences cloned into 33F1 as originating from the FAS1 gene and those DNA sequences in 102B5, from the FAS2 gene.
