1.
J Chromatogr
; 496(2): 365-75, 1989 Nov 24.
Artigo
em Inglês
| MEDLINE
| ID: mdl-2613840
RESUMO
The 2-methyl derivatives of tamoxifen (2-methyltamoxifen and 2-methyl-4-hydroxytamoxifen) were extracted from a cell culture medium at pH 5.4 (Earle's Minimum Essential Medium) with an internal standard (tamoxifen) on a phenyl sorbent cartridge. The compounds were then separated by high-performance liquid chromatography on a nitrile column eluted with acetonitrile-methanol-0.05 M sodium dihydrogenphosphate (19:4:11.6:69,v/v) containing 0.11 mmol/l disodium EDTA and determined by electrochemical detection at +1.1 V vs. Ag/AgCl/3 M NaCl. The absolute detection limits were 50 pg for 2-methyl-4-hydroxytamoxifen and 100 pg for tamoxifen and 2-methyltamoxifen at a sensitivity of 1 nA/V.
Assuntos
Tamoxifeno/análise , Neoplasias da Mama/metabolismo , Cromatografia Líquida de Alta Pressão , Meios de Cultura , Eletroquímica , Humanos , Concentração de Íons de Hidrogênio , Concentração Osmolar , Espectrofotometria Ultravioleta , Tamoxifeno/análogos & derivados , Tamoxifeno/farmacocinética , Células Tumorais Cultivadas/metabolismo
2.
Ann Pharm Fr
; 45(4): 307-13, 1987.
Artigo
em Francês
| MEDLINE
| ID: mdl-3445993