RESUMO
Heavy metal pollution in water is a serious environmental concern. Lead is one of the highly toxic heavy metals, and its toxicity is due to its non-degradation characteristic in living organisms. The monitoring of Pb(II) ions concentrations in drinking water is essential for human health and a safe environment. In this work, bio-modified carbon paste electrodes were constructed with live cells and thermally dried biomass (extracted protein) from Pseudomonas aeruginosa cells and employed for the detection of Pb(II) ions from wastewater. The biomass and biosensor preparation and optimization, and the performance of modified biosensors in the detection of Pb(II) ions are discussed here. The effect of various parameters, like pH, biomass composition, scan rate, and metal ion concentration, were studied to obtain the best electrochemical response. Further, the active surface of the electrode and solution characteristics were studies using chronocoulometry and electrochemical impedance spectroscopy. Fourier transform infrared spectroscopy (FTIR) analysis was done to find the functional groups present in the thermally dried biomass. From the present study, it is clear that the thermally dried protein biomass electrode has more sensitivity than the bare carbon paste electrode.
Assuntos
Carbono , Águas Residuárias , Eletrodos , Humanos , Íons , ChumboRESUMO
A new series of [4-(2-phenylethenesulfonylmethyl)phenyl]quinazolin-4-yl-amines was prepared and tested for its in vitro cytotoxic activity against a panel of 12 human cancer cell lines. Compounds 9, 15, 24 and 31 showed good in vitro activity and were further tested for their in vivo efficacy in the HT-29 human colon adeno carcinoma xenograft model. Compound 9 exhibited promising activity in this model. Dose-response studies for this compound against HT-29 human colon adeno carcinoma xenografts at 100, 200 and 400mg/kg doses were performed.
Assuntos
Antineoplásicos/administração & dosagem , Antineoplásicos/síntese química , Quinazolinas/administração & dosagem , Quinazolinas/síntese química , Administração Oral , Animais , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos/métodos , Feminino , Células HT29 , Humanos , Camundongos , Camundongos Nus , Ensaios Antitumorais Modelo de Xenoenxerto/métodos , Ensaios Antitumorais Modelo de Xenoenxerto/estatística & dados numéricosRESUMO
A series of 6,7-diphenyl-2,3,8,8a-tetrahydro-1H-indolizin-5-one analogues were synthesized and evaluated for cytotoxic activity against eight human cancer cell lines. Compounds 18, 21, 28, 29, 30 and 31 showed cytotoxic activity with GI(50) values in the range of 2.1-8.1 microM concentration. Among these, compounds 21 and 28 exhibited good pharmacokinetic properties. These compounds were further evaluated for their in vivo efficacy in modified hollow fibre assay (HFA).