[Stability of erythrocyte and antioxidant capacity with obese rats which were supplied of vitamin E and magnesium].

OBJECTIVE: To analyze the effect of vitamin E( Vit E) and magnesium( Mg) supplementation on stability of erythrocyte and antioxidant capacity in obese rats. METHODS: Seventy Wistar rats were randomly divided into five groups. Each group was supplied defferent doses of Vit E and Mg. All rats were sacrificed after 67 weeks. Erythrocyte osmotic fragility, fluidity of erythrocyte membrane, erythrocytic hemolyses, superoxide dismutase( SOD), malondialdehyde( MDA) and glutathione peroxidase( GSH-Px) were detected. Results of each group were analyzed by One-Way ANOVA andanalysis of variance of factorial design. RESULTS: The values of fluorescence polarization( P) and microviscosity( η) of obese model were( 0. 096 ± 0. 024) and( 0. 543 ±0. 179), respectively, which were significantly higher than other groups( P < 0. 05), it could indicate the fluidity of erythrocyte membrane of obese model was lower than other goups. The erythrocytic hemolyses of obese model was( 33. 57 ± 7. 50) %. Compared with other groups, it was increased by 11. 18%, 10%, 11. 4% and 18. 13%, respectively. The level of SOD of obese model was( 97. 54 ± 24. 81) U/mL which was lower than other groups( P < 0. 05). The level of MDA of obese model was( 17. 18 ± 12. 73) nmol/mL which was higher than other groups( P < 0. 05). Compared with normal controls and obese model, the level of GSH-Px of the group which was adding vitamin E was increased by859. 26 U, 955. 42 U, respectively. CONCLUSION: Both of Vit E and Mg could improve fluidity of erythrocyte membrane and erythrocytic hemolyses of obese rats. Combined use of Vit E and Mg could effectively improve fluidity of erythrocyte membrane. Vit E or Mg can improve the antioxidant capacity in obese rats.

[Effects of vitamin E and magnesium on glucolipid metabolism in obese rats ].

OBJECTIVE: To investigate the effects of vitamin E (VE ) and magnesium entantion on glucolipid metabolism in obese rats. METHODS: Seventy-four rats were randomly divided into five groups (normal control group positive E group, Mg group, VE plus Mg group). The doses of V(E) and Mg were 0.23 g/kg feed and 0.31 g/kg feed respectively. They were slaughtered after feed for 67 weeks. Calculate the lipid ratio. Examined the level of serum lipid, plasma glucose, serum insulin, insulin sensitivity index, and the activity of hexokinase and pyruvate kinase. The results of each group were analyzed by One-Way ANOVA and analysis of variance of factorial design. RESULTS: The rat model with nutritional obesity was successfully established that the weight of positive control group was about 20% higher than normal control group. The lipid ratio and plasma triglyceride of VE plus Mg group were 13. 29% and 0. 6 mmol/L respectively, which were significantly lower than that of positive control group (17.24% and 1. 18 mmol/L) (P < 0.05), and the plasma triglyceride of VE plus Mg group was significantly lower than that of VE group (1. 07mmol/ L) (P <0.05). The activity of hexokinase of V~E group and VE plus Mg group were 63. 67 U/L and 64. 61 U/L respectively, significantly higher than that of positive control group (42.79 U/L) and Mg group (44.02 U/L) (P <0.05). CONCLUSION: Supplementation of VE combined with Mg can effectively improve the fat content and triglyceride of obese rats, better than VE alone. Supplementation of VE and Mg is beneficial to obese rats to improve the activity of hexokinase, and adding VE is better than Mg alone.

[Correlation of band 3 protein in erythrocytes with physiological status of middle-aged and elderly people].

OBJECTIVE: To analyze the correlation of band 3 protein of erythrocytes with physiological conditions of middle-aged and elderly people, and to investigate the expression of band 3 protein under different physiological status. METHODS: Cluster randomized sampling method for selecting subjects . Physical examination data and blood samples of 218 middle-aged and elderly people (80 males and 138 females) were collected. SDS-polyacrylamide gel electrophoresis was used to analyze band 3 protein in erythrocyte membrane. The difference of band 3 protein in different groups was analyzed by T Test and One-Way ANOVA. Correlation and regression of band 3 protein with physiological status was analyzed by Pearson correlation and linear regression. RESULTS: There was a significantly positive correlation (r =0.149) between the level of band 3 protein and physical activity (P <0.05), and a negative correlation (r = -0.156) between the level of band 3 protein and systolic blood pressure (P <0.05). The correlation of band 3 protein with gender, age, BMI and diastolic blood pressure (P >0.05) is not significant. The band 3 protein level of workers with medium activity was 24. 09% , which was significantly higher than that of light activity workers (23.42%) (P <0.05). The subjects with hypertension were proved to have a significantly lower level of band 3 protein (23.33%) than normal individuals (24.20%) (P < 0.05). CONCLUSION: The level of band 3 protein was positively correlated with physical activity and negatively correlated with systolic blood pressure.