Relationship and interaction between tissue delta-like ligand 3 expression and xeroderma pigmentosum gene G gene polymorphism and sensitivity to platinum-based chemotherapy in advanced squamous lung cancer / 中国医师进修杂志

Objective:To analyze the interaction of tissue delta-like ligand 3 (DLL3) expression and xeroderma pigmentosum gene G (XPG) gene polymorphism on the sensitivity of advanced lung squamous cell carcinoma to platinum-based chemotherapy.Methods:One hundred and forty patients with advanced squamous lung cancer admitted to Yuechi County People′s Hospital from March 2019 to December 2021 were selected and all were given carboplatin and paclitaxel for injection (albumin-bound) in a fully informed manner, with one cycle every 3 weeks for a total of 4 cycles of treatment. The patients were divided into sensitive group (46 cases) and non-sensitive group (94 cases) according to their sensitivity to chemotherapy. Baseline information, tissue DLL3 expression and XPG gene polymorphism were compared between the two groups, and tissue DLL3 expression in patients with different XPG genotypes was compared. Multi-factor Logistic regression analysis was used to analyzed the factors associated with the sensitivity to chemotherapy, and interaction coefficient γ was used to analyze tissue DLL3 expression and XPG.Results:The tissue DLL3 expression score of the sensitive group was lower than that in the non-sensitive group: (3.28 ± 0.93) scores vs. (7.59 ± 1.22) scores, there was statistical difference ( P<0.01). The patients with CC genotype in the sensitive group were more than those in the non-sensitive group, and the patients with CT and TT genotypes were less than those in the non-sensitive group ( P<0.05). Tissue DLL3 expression score in patients with CC, CT, TT genotype were (3.51 ± 0.93), (6.76 ± 1.08), (10.09 ± 1.12) scores, there was statistical difference ( P<0.05); and tissue DLL3 expression score was CC<CT<TT genotype, there were significant differences between pairwise comparisons ( P<0.05). Multivariate Logistic regression analysis showed that the probability of insensitivity to platinum chemotherapy in patients with high tissue DLL3 expression was 8.368 times than that of patients with low expression, and the probability of insensitivity to platinum chemotherapy in patients with XPG genotype CT and TT was 5.349 and 9.517 times higher than that in CC genotype. The OR value caused by DLL3 alone was 6.222, the OR value caused by XPG gene polymorphism alone was 56.000, and the OR value caused by the coexistence of the two was 275.333, and the interaction coefficient γ = 1.396, indicated that the expression of tissue DLL3 was related to XPG gene polymorphism. The effect of sex had a positive interaction, and the OR value of the interaction between the two was less than the product of the OR value of the two independent factors. The model representing the interaction effect of tissue DLL3 expression and XPG gene polymorphism on chemotherapy sensitivity was a submultiplicative model. Conclusions:Patients with advanced squamous lung cancer of the CC genotype at the XPG C46T locus are more sensitive to platinum-based chemotherapy than patients of the CT and TT types, and are associated with platinum-based chemotherapy responsiveness. High tissue DLL3 expression has a positive interaction with the effect of the XPG gene polymorphism, and the two are consistent with a submultiplicative model, which may be a genetic mechanism for poor response to platinum-based chemotherapy.

Experiences in the treatment of tendinous mallet finger / 中华整形外科杂志

Objective@#To explore the effect of reconstructing the finger extension function by using partial flexor digitorum profundus tendon transposition.@*Methods@#Twelve patients were treated by using the partial proximal end of the tendon core of flexor digitorum profundus tendon regionⅠand part of region Ⅱ to cut off to the base of the distal digital phalanx, and puncturing at the end of the phalanx with a 2.0 drill. The tendon strips were pierced to the back overlap sutured to the proximal end of the avulsion of the extensor tendon .@*Results@#In 3-18 month follow-up, the average follow-up duration was 10.6 months. According to Dargan function evaluation method, 12 cases were excellent and one case was poor.@*Conclusions@#The method of reconstructing the extensor tendon by partial flexor deep tendon is a reliable method for the treatment of Mallet finger.

Design and application of fistula gauze packing drainage device / 中国中西医结合急救杂志

Gauze packing and drainage is a routine treatment for various types of fistula and sinus pressure ulcers. Because the external orifice of pressure ulcer or fistula is relatively small and the sinus deep, the conventional gauze packing has many drawbacks. Under the situation the operator is not skillful enough and no appropriate tools, it is necessary to pull the external orifice by tweezers to dilate the sinus and perform gauze packing and drainage, which brings great pain to patients, possibly leading to wound enlargement and delayed healing. At present, there is lack of auxiliary tools to improve gauze packing. Therefore, we developed and designed a gauze filler for packing and drainage of various kinds of fistula and sinus pressure ulcers, the device has the following advantages: good drainage effect, simple operation, low cost, safety and effectiveness. It not only improves the comfort of patients, reduces the number of dressing changes, reduce the number of dressing changes, shorten the hospitalization time and pay expense, but also reduces the burden of medical workers thus it is worthwhile to popularize and apply the newly designed gauze filler in clinical practice.

Research progress of molecular biology in age estimation / 中国法医学杂志

Age estimation was one of the important and difficult contents in forensic medicine research and practice. Age estimation was mainly based on bone age and dental age in present. However, the errors were large, and it was influenced by other factors. In recent years, following with the development of molecular biology theories and techniques, researchers have found that some cellular and molecular levels were related to age. In this review, the research progress of molecular biology in age estimation was reviewed.

Regneration of degenerated intervertebral dise with bone marrow mesenchymal stem cells: current status / 国际外科学杂志

Bone marrow mesenchymal stem cells (BMSCs) is a good seed cell,and it is widely used in intervertebral disc tissue engineering research.The improvement of cellular scaffolds,cell factors and further understanding of intervertebral disc (IVD) cell pathophysiology and BMSCs biology have laid the foundation for cell-based therapies for IVD degeneration.However,BMSCs repairing IVD is still in the early stage of research,at present there are still many problems,one of the most prominent is the survival of transplanted cells.This review mainly introduces the current research status of BMSCs in IVD tissue engineering.

Minimally invasive surgery for degenerative lumbar spine stenosis / 中华骨科杂志

ObjectiveTo explore the ideal minimally invasive surgical method for degenerative lumbar spine stenosis.MethodsFrom March 2008 to August 2010,73 cases with lumbar spinal stenosis underwent minimal invasive surgery were retrospectively analyzed.The patients were divided into different groups by clinical features,imaging manifestations,and concurrent diseases.The minimal invasive surgical methods were chosen according to the classification.Operation time,intra-operative bleeding,and complications were recorded.The lumbar function was evaluated by Oswestry disability index (ODI),and the clinical results were assessed by JOA pre- and postoperatively.ResultsMinimal invasive surgery was completed in all cases,which included 25 cases with bilateral decompression and 48 cases with unilateral approach for bilateral decompression.Twenty-three cases used interbody fusion and percutaneous pedicle screw fixation after decompression.Intraoperative blood loss and operation time were related to surgical methods.One case was complicated with dural tear.Cage was inserted into upper vertebral body during interbody fusion in 1case with osteoporosis,removed the cage and implanted bone fusion.Incisions of 3 cases were poor healing after surgery.The average follow up time was 13 months(range,10-35).JOA score and ODI before surgery were 9.2±5.1 and 62.3％±18.5％ respectively; while 6 months after surgery,JOA score and ODI were 23.5±7.2 and 18.4％±6.4％ respectively.JOA score and ODI showed statistically significant improvements after operation(P＜0.01).Twenty-two cases got solid fusion at the final follow-up.ConclusionTreatment of lumbar spinal stenosis by minimal invasive surgery has satisfactory surgical outcomes,but the proper minimal invasive strategy should be chosen according to specific patients,surgeons and hospitals.