RESUMO
We report the identification of a novel major histocompatibility complex class I-related chain (MICB) allele, provisionally designated as MICB-0114 pending the WHO Nomenclature Classification for the MICB locus. This new allele is identical to MICB-0103101v except for a single mutation of G to A in exon 4 that translates into an amino acid substitution from glutamic acid to lysine.
Assuntos
Genes MHC Classe I , Proteínas/genética , Alelos , Substituição de Aminoácidos , Sequência de Bases , DNA/genética , Primers do DNA/genética , Éxons , Antígenos de Histocompatibilidade Classe I , Humanos , Dados de Sequência Molecular , Mutação Puntual , Reação em Cadeia da Polimerase , Homologia de Sequência do Ácido NucleicoRESUMO
We have identified a novel MICA allele, MICA*051, detected by the polymerase chain reaction using sequence-specific primers and characterized by sequence-based typing. MICA*051 appears to be the result of a recombination between MICA*00801 and MICA*00701 at intron 2.
Assuntos
Antígenos de Histocompatibilidade Classe I/genética , Alelos , Sequência de Bases , Primers do DNA , Éxons , Humanos , Íntrons , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polimorfismo GenéticoRESUMO
The presence of an unusual HLA class I reactivity pattern was detected in a Caucasoid-Asian individual by PCR-sequence specific primer (PCR-SSP) typing. Exons 2 and 3 were characterized using PCR-sequence-based typing (PCR-SBT) and were found to contain a novel Cw*03 sequence, Cw*0315. In the region studied, Cw*0315 was comprised mainly of the Cw*0302 sequence, but at four positions it contained nucleotides normally only found in other HLA Cw locus alleles. These positions each resulted in an amino acid substitution.