Correlations between serum sirtuin levels and cardiovascular risk factors in women with polycystic ovary syndrome.

PURPOSE: Polycystic ovary syndrome (PCOS) is associated with an increased risk of cardiovascular disease (CVD) and metabolic complications often related to obesity, such as insulin resistance and type 2 diabetes mellitus (T2DM). Sirtuins (SIRTs) are nicotinamide adenine dinucleotide NAD+-dependent histone deacetylases, which modulate protein post-translational modifications (PTMs), gene transcription, increase repairing activities of DNA, and regulate metabolic processes. The aim of our study was to measure the serum levels of SIRTs 1, 2, 3, 6 and 7, and evaluate correlations between SIRTs levels and cardiovascular risk factors in women with PCOS. PATIENTS AND METHODS: The study included 54 women with PCOS and 33 healthy volunteers. Concentrations of SIRTs were measured by ELISA technique. RESULTS: Mean serum levels of SIRTs did not differ significantly between PCOS and controls. SIRTs 1, 2, and 6 positively correlated with HDL, while SIRTs 2 and 6 negatively correlated with LDL cholesterol. Negative correlation between SIRT 3 and HOMA-IR and negative correlations of SIRT 1 and 2 with insulin 120' in oral glucose tolerance test (OGTT) were demonstrated. A positive correlation between BMI and SIRT 7 concentration, and a positive correlation between body weight and SIRT 7 concentration were verified in the study group. The observed correlations between concentrations of SIRTs and metabolic parameters may indicate the involvement of these factors in the development of cardiometabolic complications. CONCLUSIONS: The results may indicate the involvement of SIRTs in the development of cardiometabolic complications. However, additional studies are required to validate this observation.

[Quality of life in patients with orbitopathy - single center experience]. / Jakosc zycia chorych z orbitopatia tarczycowa.

Thyroid-associated orbitopathy is a syndrome of eye symptoms produced by immunological inflammation of soft tissues of orbit,mostly due to Graves-Basedow disease. This disease is accompanied by proptosis, oedema of lids, double vision, tearing and photophobia. These symptoms lead to significant deterioration of quality of life due to reduction of sharpness of sight and therefore worsen emotional condition of patients. AIM: The aim of study was to assess the impact of Graves orbitopathy on quality of life. Additional aim was assessement of deppression among the subjects. MATERIALS AND METHODS: Study in form of anonymous modified questionnaire EUGOGO grading quality of life of patients with thyroidassociated orbitopathy and the scale of depression by Beck - carried out directly among 30 patients of Clinic od Endocrinology of the Medical Univeristy of Lódz (22 female and 8 male) from November 2011 to April 2012. RESULTS: Patients with thyroid-associated orbitopathy have considerably reduced quality of life - carried out qestionnaires showed they have problems with everyday activity like: riding bicycle (17 patients - 57%), driving car (14 patients - 45%), moving in apartment and outside (9 patients - 30%), watching TV (21 patients - 17%) and reading books (25 patients - 83%). Furthermore, many patients with thyroidassociated orbitopathy have serious emotional disorders - 20 persons have symptoms of depression. CONCLUSIONS: Graves orbitopathy have negative influence on quality of life. Orbitopathy worsens self-evaluation of patients, their interpersonal contacts, making new acquaintances, working and finding new job. Furthermore, many patients with thyroid-associated orbitopathy have serious emotional disorders. It seems reasonable to include psychotherapist in the therapeutic process.