Evaluation of gingival crevicular fluid interleukin-35 as a marker for identification of periodontal disease activity.

BACKGROUND: This study was carried out to evaluate and compare the levels of IL-35 in gingival crevicular fluid (GCF) in periodontally healthy subjects, patients with gingivitis and chronic periodontitis and to assess IL-35 as a marker for identification of periodontal disease activity. METHODS: GCF samples were obtained from periodontally healthy subjects (N.=15), gingivitis patients (N.=15) and patients with chronic periodontitis (N.=15). Clinical measurements like probing pocket depth, clinical attachment loss, bleeding on probing, Papillary Bleeding Index, and Modified Plaque Index were recorded. Enzyme-linked immunosorbent assay was used for the determination of GCF IL-35 levels in samples. RESULTS: The IL-35 levels were significantly higher in the healthy subjects as compared to gingivitis and chronic periodontitis group. There was variation in GCF IL-35 levels in healthy sites in each group and gingivitis sites in gingivitis and chronic periodontitis patients. CONCLUSIONS: The levels of IL-35 were observed to decrease with increase in the inflammatory status, so it might play a role in suppressing gingival inflammation and maintaining periodontal health.

Bone allograft block using demineralized freeze-dried bone ring technique in Indian patients.

Our study aimed to evaluate the effectiveness of the bone ring technique for ridge augmentation using Demineralized Freeze - Dried Bone Allograft block in Siebert's class II/class III defects along with simultaneous implant placement. A total of 15 partially edentulous patients (16 surgical sites) with Siebert's class II and/or class III defects in the anterior region of both jaws requiring ridge augmentation along with implant placement were selected. Starting from the first stage, surgery (Ridge augmentation+ implant placement) was done in the first month. Then, with continuous follow-ups and radiographic assessment, after 6 months of 2nd stage surgery was done, the implant was loaded with the final restoration. Significant results were revealed with all the parameters other than keratinized gingival and peri-implant mucosa thickness. With the mean bone resorption of 1.22 mm and 1.17 mm at the mesial and distal site at a 6-month interval, the success rate of the bone ring technique was 93.75%. The allograft bone ring technique showed a favorable outcome for the reconstruction of large vertical defects.