RESUMO
Some clinical characteristics of cord blood transplantation (CBT) might be explained by specificities in the reconstitution of immune subsets differing by their maturation stage or their implication in GVHD, tolerance or immune responses against tumor or infectious agents. Here, we compare the immune reconstitution of several of these subsets after CBT and BMT. B-cell count recovery was faster after CBT. There was no difference in the recovery of CD4(+) and CD8(+) cell counts. There was no difference either in the frequency of several subsets: CD45RO(+) memory, and CD45RA(+) naïve cells within the CD4(+) T-cell compartment, CD27(+) among B cells, CD56(bright), NKG2A(+), and KIR(+) cells among natural killer (NK) cells, CD25(+)FOXP3(+) regulatory T cells and invariant NKT cells. The proportion of the thymic naïve CD31(+)CD45RA(+)CD4(+) T cells was lower after CBT at 6 months post-transplant, and was still below normal at 1 year in both groups. NK-cell expansion was more sustained after CBT, with fewer double-negative NKG2A(-)KIR(-) hyporesponsive cells and more double-positive NKG2A(+)KIR(+) hyper-responsive NK cells. These results, therefore, indicate that further research to improve CBT outcome should try to improve thymopoieisis and take advantage of the sustained NK-cell reconstitution.
Assuntos
Transplante de Medula Óssea/métodos , Sangue Fetal/transplante , Leucemia/cirurgia , Subpopulações de Linfócitos/citologia , Linfócitos/citologia , Linfócitos T Reguladores/citologia , Adolescente , Criança , Estudos de Coortes , Feminino , Sangue Fetal/imunologia , Humanos , Células Matadoras Naturais/citologia , Células Matadoras Naturais/imunologia , Leucemia/sangue , Leucemia/patologia , Linfócitos/imunologia , Masculino , Linfócitos T Reguladores/imunologiaRESUMO
The Unc-33-like phosphoprotein/collapsin response mediator protein (Ulip/CRMP) family consists of four homologous phosphoproteins considered crucial for brain development. Autoantibodies produced against member(s) of this family by patients with paraneoplastic neurological diseases have made it possible to clone a fifth human Ulip/CRMP and characterize its cellular and anatomical distribution in developing brain. This protein, referred to as Ulip6/CRMP5, is highly expressed during rat brain development in postmitotic neural precursors and in the fasciculi of fibers, suggesting its involvement in neuronal migration/differentiation and axonal growth. In the adult, Ulip6/CRMP5 is still expressed in some neurons, namely in areas that retain neurogenesis and in oligodendrocytes in the midbrain, hindbrain, and spinal cord. Ulip2/CRMP2 and Ulip6/CRMP5 are coexpressed in postmitotic neural precursors at certain times during development and in oligodendrocytes in the adult. Because Ulip2/CRMP2 has been reported to mediate semaphorin-3A (Sema3A) signal in developing neurons, in studies to understand the function of Ulip6/CRMP5 and Ulip2/CRMP2 in the adult, purified adult rat brain oligodendrocytes were cultured in a Sema3A-conditioned medium. Oligodendrocytes were found to have Sema3A binding sites and to express neuropilin-1, the major Sema3A receptor component. In the presence of Sema3A, these oligodendrocytes displayed a dramatic reduction in process extension, which was reversed by removal of Sema3A and prevented by anti-neuropilin-1, anti-Ulip6/CRMP5, anti-Ulip2/CRMP2 antibodies, or VEGF-165, another neuropilin-1 ligand. These results indicate the existence in the adult brain of a Sema3A signaling pathway that modulates oligodendrocyte process extension mediated by neuropilin-1, Ulip6/CRMP5, and Ulip2/CRMP2, and they open new fields of investigation of neuron/oligodendrocyte interactions in the normal and pathological brain.
Assuntos
Glicoproteínas , Glicoproteínas/metabolismo , Proteínas do Tecido Nervoso/biossíntese , Proteínas do Tecido Nervoso/metabolismo , Oligodendroglia/metabolismo , Animais , Anticorpos/farmacologia , Encéfalo/embriologia , Encéfalo/crescimento & desenvolvimento , Encéfalo/metabolismo , Células Cultivadas , Fatores de Crescimento Endotelial/farmacologia , Feminino , Glicoproteínas/farmacologia , Humanos , Hidrolases , Imuno-Histoquímica , Hibridização In Situ , Peptídeos e Proteínas de Sinalização Intercelular , Linfocinas/farmacologia , Masculino , Camundongos , Proteínas Associadas aos Microtúbulos , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/antagonistas & inibidores , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/isolamento & purificação , Neuritos/efeitos dos fármacos , Neuritos/metabolismo , Neuropilina-1 , Oligodendroglia/citologia , Oligodendroglia/efeitos dos fármacos , Especificidade de Órgãos , Fosfoproteínas/biossíntese , Fosfoproteínas/genética , Fosfoproteínas/isolamento & purificação , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Ratos , Semaforina-3A , Homologia de Sequência de Aminoácidos , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Fator A de Crescimento do Endotélio Vascular , Fatores de Crescimento do Endotélio VascularRESUMO
Presentamos el caso de una paciente de 65 años con disgnóstico de dermatosis papulosa blanca de cuello. Efectuamos comentarios del caso a la luz de literatura en los aspectos clínicos, patológicos y terapéuticos(AU)
Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Dermatoses Faciais/terapiaRESUMO
Presentamos el caso de una paciente de 65 años con disgnóstico de dermatosis papulosa blanca de cuello. Efectuamos comentarios del caso a la luz de literatura en los aspectos clínicos, patológicos y terapéuticos
Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Dermatoses Faciais/terapiaRESUMO
La histoplasmosis ha sido incluida como enfermedad marcadora de SIDA en 1985. En nuestro medio iguala en su incidencia a la criptococosis. Se presenta un caso de histoplasmosis diseminada subaguda con lesiones cutáneas generalizadas y estado general conservado. Se arribó a su diagnóstico luego de múltiples estudios. Fue la primera infección oportunista y la que marcó el diagnóstico de SIDA
Assuntos
Humanos , Masculino , Adulto , Histoplasmose/diagnóstico , Histoplasmose/tratamento farmacológico , Histoplasmose/patologia , Síndrome da Imunodeficiência Adquirida/complicaçõesRESUMO
La histoplasmosis ha sido incluida como enfermedad marcadora de SIDA en 1985. En nuestro medio iguala en su incidencia a la criptococosis. Se presenta un caso de histoplasmosis diseminada subaguda con lesiones cutáneas generalizadas y estado general conservado. Se arribó a su diagnóstico luego de múltiples estudios. Fue la primera infección oportunista y la que marcó el diagnóstico de SIDA (AU)
