Comparative study on packing materials for improved biological methanation in trickle Bed reactors.

Packing materials improve biological methanation efficiency in Trickle Bed Reactors. The present study, which lies in the field of energy production and biotechnology, entailed the evaluation of commercial pelletized activated carbon and Raschig rings as packing materials. The evaluation focused on monitoring process indicators and examining the composition of the microbial community. Activated carbon resulted in enhanced methane purity, achieving a two-fold higher methane percentage than Raschig rings, maintaining a stable pH level within a range of 7-8 and reducing gas retention time from 6 h to 90 min. Additionally, the digestate derived from biogas plant was found to be a sufficient nutrient source for the process. Fermentative species with genes for ß-oxidation, such as Amaricoccus sp. and Caloramator australicus could explain the production of hexanoic and valerate acids during reactor operation. Based on the physical properties of packing materials, the efficiency of biological methanation could be maximized.

High-resolution 3D imaging uncovers organ-specific vascular control of tissue aging.

Blood vessels provide supportive microenvironments for maintaining tissue functions. Age-associated vascular changes and their relation to tissue aging and pathology are poorly understood. Here, we perform 3D imaging of young and aging vascular beds. Multiple organs in mice and humans demonstrate an age-dependent decline in vessel density and pericyte numbers, while highly remodeling tissues such as skin preserve the vasculature. Vascular attrition precedes the appearance of cellular hallmarks of aging such as senescence. Endothelial VEGFR2 loss-of-function mice demonstrate that vascular perturbations are sufficient to stimulate cellular changes coupled with aging. Age-associated tissue-specific molecular changes in the endothelium drive vascular loss and dictate pericyte to fibroblast differentiation. Lineage tracing of perivascular cells with inducible PDGFRß and NG2 Cre mouse lines demonstrated that increased pericyte to fibroblast differentiation distinguishes injury-induced organ fibrosis and zymosan-induced arthritis. To spur further discoveries, we provide a freely available resource with 3D vascular and tissue maps.

Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease.

Bone vasculature and bone marrow vascular niches supply oxygen, nutrients, and secrete angiocrine factors required for the survival, maintenance, and self-renewal of stem and progenitor cells. In the skeletal system, vasculature creates nurturing niches for bone and blood-forming stem cells. Blood vessels regulate hematopoiesis and drive bone formation during development, repair, and regeneration. Dysfunctional vascular niches induce skeletal aging, bone diseases, and hematological disorders. Recent cellular and molecular characterization of the bone marrow microenvironment has provided unprecedented insights into the complexity, heterogeneity, and functions of the bone vasculature and vascular niches. The bone vasculature is composed of distinct vessel subtypes that differentially regulate osteogenesis, hematopoiesis, and disease conditions in bones. Further, bone marrow vascular niches supporting stem cells are often complex microenvironments involving multiple different cell populations and vessel subtypes. This review provides an overview of the emerging vascular cell heterogeneity in bone and the new roles of the bone vasculature and associated vascular niches in health and disease. © 2020 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).