Carboxy-PEG-thiol functionalized gold nanoparticle conjugates for the detection of SARS-CoV-2: detection tools and analytical method development.

Addressing the need for accessible SARS-CoV-2 testing, carboxy-PEG 12-thiol functionalized gold nanoparticles conjugates were developed for rapid point-of-care (POC) detection against SARS-CoV-2 spike protein, pseudo-SARS-CoV-2, and authentic Beta SARS-CoV-2 virus particles. These conjugates leverage gold nanoparticles (AuNPs) as signal transducers, cross-linked to either angiotensin-converting enzyme 2 (ACE2) or SARS-CoV-2 spike protein receptor-binding domain (RBD) antibodies as bioreceptors and showed a distinct color shift from pink to blue. To assess their POC feasibility, the conjugates were integrated into facemasks and breathalyzers, wherein aerosolized SARS-CoV-2 antigens were successfully detected, producing a color change within 10 and 30minutes for the breathalyzer and facemask prototypes, respectively. Furthermore, we explored quantitative analysis using varying concentrations of SARS-CoV-2 spike protein. Both conjugates demonstrated a linear relationship between blue color intensity and virus concentration, with linear ranges of 0.08-0.6ng/mL and 0.04-0.5ng/mL, respectively. Low limits of detection and quantification were also achieved. They exhibited specificity, responding solely to SARS-CoV-2 even in complex matrices containing diverse proteins, including the SARS-CoV-1 spike protein. Precision tests yielded coefficient of variations below 2%, showcasing their remarkable reproducibility. This work presents a promising approach for rapid, sensitive, and specific POC detection of SARS-CoV-2 paving the way for improved pandemic response and management.

Synthesis and characterization of polyaniline, polypyrrole and zero-valent iron-based materials for the adsorptive and oxidative removal of bisphenol-A from aqueous solution.

One pot synthesis of a polypyrrole, polyaniline and Fe0 nano-composite (Fe0-PPY/PANI) was achieved by polymerizing aniline and pyrrole with FeCl3 followed by the reduction of Fe3+ to Fe0 with NaBH4. PPY/PANI was synthesized the same way as Fe0-PPY/PANI, except that all the FeCl3 was removed by rinsing. The presence of Fe0 was demonstrated using several analytical techniques; this was shown in comparison to materials that are without Fe0. A series of materials were screened as both adsorbents and catalyst for the activation of H2O2 towards bisphenol A (BPA) removal in batch experiments. Polymers performed better than composites containing Fe0 at adsorption, whereas Fe0 based materials were better catalysts for the activation of H2O2. BPA samples were then spiked with other contaminants including sewage water to test the performance of the various adsorbents and Fenton catalysts. PPY/PANI was found to be a better adsorbent than the rest, whereas Fe0-PPY/PANI was the best Fenton catalyst. The adsorption kinetics of BPA onto PPY/PANI was studied; it was found that the process was governed by the pseudo-second-order kinetic model. The adsorption isotherms revealed that the amount of BPA taken up by PPY/PANI increased with increasing temperature and was governed by the Langmuir adsorption isotherm. The mechanism in which Fe0-PPY/PANI and H2O2 degraded BPA was studied, it was found that surface-bound hydroxyl radicals were responsible for the degradation of BPA. It was also shown that the degradation process included the formation of smaller compounds leading to the reduction of the total organic content by 57%.

Resin-gel incorporation of high concentrations of W6+ and Zn2+ into TiO2-anatase crystal to form quaternary mixed-metal oxides: effect on the a lattice parameter and photodegradation efficiency.

The search for a viable photocatalyst for water remediation is ongoing and in recent times the efforts have predominantly focused on improving the limitations of the TiO2 photocatalyst. This paper reports a dual strategy for improving the photocatalytic properties of TiO2. The first strategy is to dope up to 30% of W6+ and Zn2+ into the crystal lattice of TiO2 using the resin gel technique to synthesize quaternary mixed metal oxides (QMMOs). It was demonstrated by laser Raman spectroscopy, PXRD and various other strategies, including dislodging the dopants from the crystal lattice of TiO2, that these materials were successfully synthesized. More importantly, UV-DRS showed that these materials could absorb visible light. TiO2 and the QMMOs were also supported on 10% NCNTs synthesized from coal fly ash, by slightly modifying the resin gel technique. It was observed from TEM images that the NCNTs were uniformly coated with TiO2 and QMMO nanoparticles. These composites were observed to have lower photoluminescence emission spectra when compared to neat TiO2 and unsupported QMMOs. The two-part strategy employed in this project worked as the QMMOs supported on 10% NCNTs had higher visible light photodegradation efficiencies compared to neat TiO2 and the unsupported QMMOs.

Remarkable sensitivity for detection of bisphenol A on a gold electrode modified with nickel tetraamino phthalocyanine containing Ni-O-Ni bridges.

This work reports the electrocatalysis of bisphenol A on Ni(II) tetraamino metallophthalocyanine (NiTAPc) polymer modified gold electrode containing Ni-O-Ni bridges (represented as Ni(OH)TAPc). The Ni(II)TAPc films were electro-transformed in 0.1 mol L(-1) NaOH aqueous solution to form 'O-Ni-O oxo bridges', forming poly-n-Ni(OH)TAPc (where n is the number of polymerising scans). poly-30-Ni(OH)TAPc, poly-50-Ni(OH)TAPc, poly-70-Ni(OH)TAPc and poly-90-Ni(OH)TAPc films were investigated. The polymeric films were characterised by electrochemical impedance spectroscopy and the charge transfer resistance (R(CT)) values increased with film thickness. The best catalytic activity for the detection of bisphenol A was on poly-70-Ni(OH)TAPc. Electrode resistance to passivation improved with polymer thickness. The electrocatalytic behaviour of bisphenol A was compared to that of p-nitrophenol in terms of electrode passivation and regeneration. The latter was found to passivate the electrode less than the former. The poly-70-Ni(OH)TAPc modified electrode could reliably detect bisphenol A in a concentration range of 7x10(-4) to 3x10(-2)mol L(-1) with a limit of detection of 3.68x10(-9)mol L(-1). The sensitivity was 3.26x10(-4)A mol(-1) L cm(-2).