RESUMO
The anemia of inflammation (AI) is characterized by the presence of inflammation and abnormal elevation of hepcidin. Accumulating evidence has proved that Rocaglamide (RocA) was involved in inflammation regulation. Nevertheless, the role of RocA in AI, especially in iron metabolism, has not been investigated, and its underlying mechanism remains elusive. Here, we demonstrated that RocA dramatically suppressed the elevation of hepcidin and ferritin in LPS-treated mice cell line RAW264.7 and peritoneal macrophages. In vivo study showed that RocA can restrain the depletion of serum iron (SI) and transferrin (Tf) saturation caused by LPS. Further investigation showed that RocA suppressed the upregulation of hepcidin mRNA and downregulation of Fpn1 protein expression in the spleen and liver of LPS-treated mice. Mechanistically, this effect was attributed to RocA's ability to inhibit the IL-6/STAT3 pathway, resulting in the suppression of hepcidin mRNA and subsequent increase in Fpn1 and TfR1 expression in LPS-treated macrophages. Moreover, RocA inhibited the elevation of the cellular labile iron pool (LIP) and reactive oxygen species (ROS) induced by LPS in RAW264.7 cells. These findings reveal a pivotal mechanism underlying the roles of RocA in modulating iron homeostasis and also provide a candidate natural product on alleviating AI.
Assuntos
Hepcidinas , Homeostase , Interleucina-6 , Ferro , Lipopolissacarídeos , Receptores da Transferrina , Fator de Transcrição STAT3 , Hepcidinas/metabolismo , Hepcidinas/genética , Animais , Camundongos , Ferro/metabolismo , Células RAW 264.7 , Receptores da Transferrina/metabolismo , Receptores da Transferrina/genética , Lipopolissacarídeos/farmacologia , Interleucina-6/metabolismo , Interleucina-6/genética , Fator de Transcrição STAT3/metabolismo , Fator de Transcrição STAT3/genética , Espécies Reativas de Oxigênio/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Inflamação/metabolismo , Inflamação/genética , Inflamação/patologia , Transdução de Sinais/efeitos dos fármacos , Anemia/metabolismo , Anemia/genética , Anemia/tratamento farmacológico , Anemia/patologia , Ferritinas/metabolismo , Ferritinas/genética , Masculino , Fígado/metabolismo , Fígado/patologia , Macrófagos/metabolismo , Macrófagos/efeitos dos fármacos , Proteínas de Transporte de CátionsRESUMO
Objective: Using the wireless mobile communication technology and Internet technology, cloud platform to build maternal and infant health intelligent management. Methods: Using the J2EE technology based on B/S three-tier to build health intelligent management cloud platform. Vital signs parameters (such as blood pressure, blood oxygen saturation, heart rate, breathing, pulse, temperature, instantaneous heart rate of pregnant women, etc) achieved the real-time detection and wireless remote transmission. Results: Realized the digitization management of maternal health information, health records, health analysis; Through this platform to realize the consulting between the patient and doctor online. Conclusion: The Pregnant women can upload and download various vital signs parameters at any time to check the personal health analysis report through mobile phone client.
Assuntos
Telefone Celular , Monitorização Fisiológica , Sinais Vitais , Tecnologia sem Fio , Feminino , Humanos , Internet , GravidezRESUMO
An online ECG signals analysis service system based on the network has been established and is introduced in this paper which integrates the advantages of the network, database and ECG analysis tools, providing on-line ECG signal analysis service. Users with the knowledge of general operation of a browser, can process the ECG data and get a real-time display of analysis results.
