Biochar Blended with Nitrogen Fertilizer Promotes Maize Yield by Altering Soil Enzyme Activities and Organic Carbon Content in Black Soil.

Biochar and nitrogen fertilizers are known to increase soil carbon storage and reduce soil nitrogen loss as amendments, suggesting a promising strategy for highly effectively increasing soil productivity. However, few studies have explored the mechanisms of their effects on crop yield in terms of active carbon fraction and enzyme activity, which ultimately limits the potential for the application of biochar in combination with nitrogen fertilizers. To evaluate the effect of biochar and nitrogen fertilizer on the improvement of black soils in northeast China, a field experiment was conducted in the black soil to compare and analyze the application methods on total organic carbon (TOC), total nitrogen (TN), enzyme activities, and maize yields. Biochar rates: CK, C1, C2, and C3 (0, 9.8, 19.6, and 29.4 Mg·ha-1); N fertilizer rates: N1/2 and N (30 and 60 kg·ha-1). Results indicated that biochar and N fertilizer amendments significantly ameliorated soil fertility, such as TOC and TN, compared to the unamended soil. The TOC levels in the C3 treatment increased by 35.18% and the TN levels by 23.95%. The improvement in TN is more significant when biochar is blended with N fertilizer. Biochar blended with N fertilizer increased maize cellulase, urease, and invertase activities by an average of 53.12%, 58.13%, and 16.54%, respectively. Redundancy analysis showed that TOC, TN, and MBN contributed 42%, 16.2%, and 22.2%, respectively, to the maize yield indicator. Principal component analysis showed that reduced N fertilizer was more effective in improving yields, with a maximum yield increase of 50.74%. Biochar blended with N fertilizer is an effective strategy to improve the fertility and productivity of black soils in northeast China, while nitrogen fertilizer reduction is feasible and necessary for maintaining grain yield.

Effects of residual monensin in livestock manure on nitrogen transformation and microbial community during "crop straw feeding-substrate fermentation-mushroom cultivation" recycling system.

Although crop-livestock integration recycling systems improve nitrogen (N) utilization in agroecosystems, there are limited studies regarding impacts of residual antibiotics in livestock manure on N transformation in entire recycling system. The objective was to evaluate effects of feeding monensin on N recycling during "straw feeding-substrate fermentation-mushroom cultivation". This experiment contained 3 steps. During straw feeding, beef cattle were allocated into 2 groups and fed diets with or without monensin, respectively. During fermentation, beef cattle manure (with or without monensin) and straw (corn or wheat) and were co-fermented for 35 d to produce substrates. During cultivation, Agaricus bisporus was cultivated on 4 substrates to recycle N in the form of mushrooms. Rates of N retention during fermentation were significant higher for monensin and corn straw treatments and there was an significant interaction between straw and antibiotic on N retention rate during cultivation. However, residual monensin significantly reduced amount of recycled N during entire recycling system, due to changes in N transformation-associated enzyme activity, ammonification and denitrification plus microbial community structure and succession. Specifically, residual monensin inhibited growth of dominant bacterial phylum Bacteroidetes and fungal phylum Neocallimastigomycota, and increased bacterial phylla Actinobacteriota and Firmicutes. These alterations in functional microbes increased N retention rates but reduced mushroom yields in antibiotic treatments during cultivation. In conclusion, monensin decreased the N recycling rate in recycling system, but also reduced N losses during fermentation by inhibiting ammonification and denitrification, so, avoiding antibiotics usage is an effective strategy to improve the efficiency of recycling systems.

The effects of soil freeze-thaw processes on water and salt migrations in the western Songnen Plain, China.

Seasonally freeze-thaw (FT) processes affect soil salinisation in cold and arid regions. Therefore, understanding the mechanisms behind soil salinisation during winter and spring is crucial for management strategies effectively alleviating this. This study aimed to explore the soil FT characteristics and their influences on soil water and salt migrations to clarify the underlying mechanism of the springtime soil salinisation in the western Songnen Plain, China. The spatiotemporal distributions of soil water and salt, frozen depths and soil temperatures were examined at depths of 0-200 cm in three typical landscapes (farmland, Leymus chinensis (Trin.) Tzvel (LT) grassland and alkali-spot (AS) land) from October 2015 to June 2016. Results indicated that the strongest freezing process occurred in AS land, which was characterised by the deepest frost depth (165 cm) and highest freezing rate (3.58 cm/d), followed by LT grassland, and then farmland. The freeze-induced upward redistribution and enrichment of soil water and salt caused the rise and expansion of the soil salification layer, which was the main source of explosive accumulations of surface salt in springtime. Therefore, the FT processes contributed to the surface soil salinisation and alkalisation. Landscapes also affected soil water and salt migrations during FT processes, with the trend being AS land > LT grassland > farmland.

Fractal dimension of particle-size distribution and their relationships with alkalinity properties of soils in the western Songnen Plain, China.

The purpose of this study was to identify the fractal dimension and their relationships with alkalinity properties of soils, and to evaluate the potential of fractal dimension as an indicator of alkalinity properties of soil. Six soils with an increasing salinity (electrical conductivity was 0.09, 0.18, 0.62, 0.78, 1.57 and 1.99 dS m-1, respectively) were selected from the western part of the Songnen Plain (China). Salt content, exchangeable sodium percentage, sodium adsorption ratio and other properties of the soils were determined and the soil particle-size distribution (0-2000 µm) was measured using a laser diffraction particle size analyser. Our results show that the overall fractal dimension of the selected soils ranged from 2.35 to 2.60. A linear regression analysis showed a significant negative correlation between fractal dimension and the amount of coarse sand and fine sand (r = - 0.5452, P < 0.05 and r = - 0.8641, P < 0.01, respectively), and a significant positive correlation with silt and clay (r = 0.9726, P < 0.01 and r = 0.9526, P < 0.01, respectively). Thus, soils with higher silt and clay content have higher fractal dimension values. Strong linear relationships between fractal dimension and salt content (P < 0.05), in particular a very significant positive relationship with HCO3- (P < 0.01), also exist. It is therefore possible to conclude that a soil's fractal dimension could serve as a potential indicator of soil alkalization and the variability in alkaline soil texture.

[Adaptability of abnormal tadpole (Rana chensinensis) to water pH, salinity and alkalinity in Changbai Mountain of China].

Under field condition with 16-18 degree C water temperature, single-factor acute toxicity test was used to study the toxicity effects of water pH, salinity and carbonate-alkalinity on abnormal tadpole (R. chensinensis). The results showed that when the water salinity was 0.18 g x L(-1), carbonate-alkalinity was 1.41 mmol x L(-1), and water pH was 4.3-9.7, the survival rate of abnormal tadpole within 96 hours was not affected. The upper limit of LC50 for the pH within 24, 48, 72 and 96 hours was 10.33, 10.18, 10.08 and 10.02, and the prescribed minimum was 3.92, 4.07, 4.11 and 4.16, respectively. The upper limit of LC0 was 9.95, 9.80, 9.70 and 9.70, and the prescribed minimum was 4.23, 4.45, 4.30 and 4.30, and that of LC100 was 10.70, 10.55, 10.45 and 10.33, and the prescribed minimum was 3.55, 3.70, 3.92 and 4.03, respectively. The survival rate of abnormal tadpole within 96 hours was not affected in the water salinity between 2.0-3.0 g x L(-1). When water pH was 7.0-8.5 and carbonate-alkalinity was 1.41 mmol x L(-1), the LC50 of the salinity within 24, 48, 72 and 96 hours was 8.21, 7.25, 5.17 and 3.70 g x L(-1), the LC0 was 7.14, 6.00, 2.67 and 2.20 g x L(-1), and the LC100 was 9.98, 9.00, 7.67 and 5.20 g x L(-1), respectively, while the SC was 1.70 g x L(-1). Under the same water pH and when the water salinity was 0.18 g x L(-1), the LC50 of carbonate-alkalinity within 24, 48, 72 and 96 hours was 14.36, 11.83, 10.35, and 7.68 mmol x L(-1), the LC0 was 8.76, 8.51, 4.65 and 3.88 mmol x L(-1), and the LC100 was 19.96, 15.14, 16.05 and 11.48 mmol x L(-1), respectively, while the SC was 1.70 mmol x L(-1). The survival rate of abnormal tadpole (R. chensinensis) was decreased with increasing water pH, salinity and carbonate-alkalinity. The optimum water salinity and carbonate-alkalinity to the survival and the growth of abnormal tadpole (R. chensinensis) were below 2.0 g x L(-1) and 3.0 mmol x L(-1), respectively, and water pH was between 6.0 and 9.0.