Evaluation of In Vivo Folic Acid Bioavailability in Different Mouse Strains Using Enzymatic Digestion Combined with Ultra Performance Liquid Chromatography.

By analyzing the folic acid content of various mouse strains through the use of in vivo studies, this study sought to determine whether folic acid bioavailability varies between hosts. In order to examine the stability of folic acid in the gastrointestinal tract, the rate at which it enters the blood, its retention in the organs, and its entry into the brain, folic acid was gavaged for 10 days into male and female mice of the following four strains: C57BL/6, BALB/c, ICR, and Kunming. Folic acid was extracted from eight groups of mice via solid phase extraction and triple enzyme extraction; the folic acid was subsequently quantified by ultraperformance liquid chromatography. In contrast to the other groups, female C57BL/6 mice exhibited substantially greater bioavailability as well as variations in organ retention and blood entry rates, as indicated by the experimental findings. This finding indicated that using female C57BL/6 mice to evaluate the bioavailability of folic acid is more effective.

Elucidating the role of confinement and shielding effect over zeolite enveloped Ru catalysts for propane low temperature degradation.

Volatile organic compounds (VOCs) are the main precursor for ozone formation and hazardous to human health. Light alkane as one of the typical VOCs is difficult to degrade to CO2 and H2O by catalytic degradation method due to its strong C-H bond. Herein, a series of ultrafine Ru nanoclusters (<0.95 nm) enveloped in silicalite-1 (S-1) zeolite catalysts were designed and prepared by a simple one-pot method and applied for catalytic degradation of propane. The results demonstrate that the enveloped Ru1@S-1 catalyst has excellent propane degradation performance. Its T95 is as low as 294 °C with moisture, and the turnover frequency (TOF) value is up to 5.07 × 10-3 s-1, evidently higher than that of the comparison supported catalyst (Ru1/S-1). Importantly, Ru1@S-1 exhibits superior thermal stability, water resistance and recyclability, which should be attributed to the confinement and shielding effect of the S-1 shell. The in-situ DRIFTS result reveals that the propane degradation over Ru1@S-1 follows the Mars-van-Krevelen (MvK) mechanism, where the hydroxy from the framework of zeolite can provide the active oxygen species. Our work provides a new candidate and guideline for an efficient and stable catalyst for the low-temperature degradation of the light alkane VOCs.