RESUMO
BACKGROUND: Implant-related infections are a challenging complication of orthopedic surgery, primarily due to the formation of bacterial biofilms on the implant surface. An antibacterial coating for titanium implants was developed to provide novel insights into the prevention and treatment of implant-related infections. METHODS: Titanium plates were coated with TiO2 nanotubes by anodization, and iodine was doped onto the coating via electrophoretic deposition. The obtained plates were characterized using a range of analytical techniques. Subsequently, Staphylococcus aureus was inoculated onto the surfaces of untreated titanium plates (control group), TiO2-nanocoated titanium plates (TiO2 group), and iodine-doped TiO2-nanocoated titanium plates (I-TiO2 group) to compare their antibacterial properties. RESULTS: Twenty-four hour in vitro antimicrobial activity test of the I-TiO2 group against Staphylococcus aureus was superior to those of the other groups, and this difference was statistically significant (P < 0.05). CONCLUSIONS: This coating technology provides a new theoretical basis for the development of anti-infective implants against Staphylococcus aureus in orthopedics.
Assuntos
Anti-Infecciosos , Iodo , Nanotubos , Infecções Estafilocócicas , Humanos , Staphylococcus aureus , Iodo/farmacologia , Titânio , Materiais Revestidos Biocompatíveis/farmacologia , Antibacterianos/farmacologia , Infecções Estafilocócicas/prevenção & controle , Propriedades de SuperfícieRESUMO
We report a case of postoperative urinary leakage after bilateral laparoscopic totally extraperitoneal (TEP) herniorrhaphy. A man in his upper 80s with a healed cystostomy and appendectomy underwent bilateral TEP herniorrhaphy. Urinary leakage was noted by ultrasound examination 4 days after bilateral TEP. Cystography and computed tomography conclusively confirmed a 6-mm extraperitoneal fistula at the site of the previous cystostomy. The fistula involved the anterior bladder wall and was associated with an extended urinoma. The patient was treated by indwelling catheterization using a Foley catheter and repeated ultrasound-guided puncture and aspiration of the inguinal effusion at the bedside. The patient was completely healed 69 days after the operation with no mesh infection or bladder dysfunction. We believe that urinary leakage is possible after TEP herniorrhaphy in patients with a healed suprapubic cystostomy. Therefore, indwelling catheterization using a Foley catheter should be implemented before surgery, and the Foley catheter can be removed within 1 week after surgery if no postoperative urinary leakage is observed. A history of suprapubic cystotomy should not be regarded as a contraindication for TEP surgery. This is the first report of urinary leakage after bilateral TEP herniorrhaphy in a patient with a healed cystostomy and appendectomy.
Assuntos
Hérnia Inguinal , Laparoscopia , Masculino , Humanos , Cistostomia , Apendicectomia/efeitos adversos , Herniorrafia , Hérnia Inguinal/cirurgia , Laparoscopia/efeitos adversos , Laparoscopia/métodos , Cateterismo Urinário , Resultado do Tratamento , Telas CirúrgicasRESUMO
MG-63 human osteosarcoma cells were transfected with short hairpin RNA (shRNA) against livin and survivin using monomethoxypolyethylene glycolchitosan (mPEGCS) nanoparticles (NPs) as carriers, with the aim of evaluating the effect on cell proliferation and apoptosis. mPEGCS NPs sized ~100 nm were prepared by ionic crosslinking. mPEGCSlivin shRNA, mPEGCSsurvivin shRNA and mPEGCS(livin shRNA + survivin shRNA) NPs were constructed by electrostatic adsorption at NP suspension/gene solution ratios of 3:1 to transfect MG63 cells. The expression levels of livin and survivin mRNA and protein were measured by reverse transcriptionpolymerase chain reaction and western blotting, respectively. The inhibitory effects of downregulated livin and survivin expression on cell proliferation were measured using an MTT assay. The apoptosisinducing effects of livin and surivin knockdown were investigated using a Hoechst staining kit. All shRNA groups resulted in reduced expression of livin and survivin mRNA and protein in MG63 cells. The MTT assay and Hoechst staining indicated that simultaneous knockdown of livin and survivin genes inhibited the proliferation of MG63 cells and promoted their apoptosis, to a greater extent than knocking down either gene individually. The simultaneous interference mediated by mPEGCS NPs significantly reduced livin and survivin expression in MG63 cells, suppressed proliferation and facilitated apoptosis, to a greater extent than knockdown of either livin or survivin alone were. Thus the results indicate a synergistic effect of livin and survivin.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Quitosana/química , Técnicas de Silenciamento de Genes , Proteínas Inibidoras de Apoptose/metabolismo , Nanopartículas/química , Proteínas de Neoplasias/metabolismo , Osteossarcoma/metabolismo , Polietilenoglicóis/química , Interferência de RNA , Apoptose , Western Blotting , Linhagem Celular Tumoral , Proliferação de Células , Forma Celular , Regulação Neoplásica da Expressão Gênica , Humanos , Osteossarcoma/genética , Osteossarcoma/patologia , RNA Mensageiro/metabolismo , RNA Interferente Pequeno/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , SurvivinaRESUMO
This paper is based upon a certain hospital's Mobile Clinical Information System. It discusses the design of the system in detail from three aspects: its function, its framework and the selection of its software and hardware. Finally some discussions are made for its improvement and perfection. PDA (personal digital assistant), clinical information, wireless network
