An 'AND'-based ratiometric fluorescence probe for the sequential detection of biothiols and hypochlorous acid.

An 'AND'-based ratiometric fluorescence probe for the sequential detection of biothiols and hypochlorous acid was developed. FRET was observed only when RSHClO reacted with biothiols before reacting with hypochlorous acid, a phenomenon that has been confirmed in aqueous solutions and cells. This feature enables the probe to mimic biological processes and is particularly suitable for imaging oxidizing and reducing substances that cannot coexist.