I/O-efficient iterative matrix inversion with photonic integrated circuits.

Photonic integrated circuits have been extensively explored for optical processing with the aim of breaking the speed and energy efficiency bottlenecks of digital electronics. However, the input/output (IO) bottleneck remains one of the key barriers. Here we report a photonic iterative processor (PIP) for matrix-inversion-intensive applications. The direct reuse of inputted data in the optical domain unlocks the potential to break the IO bottleneck. We demonstrate notable IO advantages with a lossless PIP for real-valued matrix inversion and integral-differential equation solving, as well as a coherent PIP with optical loops integrated on-chip, enabling complex-valued computation and a net inversion time of 1.2 ns. Furthermore, we estimate at least an order of magnitude enhancement in IO efficiency of a PIP over photonic single-pass processors and the state-of-the-art electronic processors for reservoir training tasks and multiple-input and multiple-output (MIMO) precoding tasks, indicating the huge potential of PIP technology in practical applications.

Integrated reconstructive spectrometer with programmable photonic circuits.

Optical spectroscopic sensors are a powerful tool to reveal light-matter interactions in many fields. Miniaturizing the currently bulky spectrometers has become imperative for the wide range of applications that demand in situ or even in vitro characterization systems, a field that is growing rapidly. In this paper, we propose a novel integrated reconstructive spectrometer with programmable photonic circuits by simply using a few engineered MZI elements. This design effectively creates an exponentially scalable number of uncorrelated sampling channels over an ultra-broad bandwidth without incurring additional hardware costs, enabling ultra-high resolution down to single-digit picometers. Experimentally, we implement an on-chip spectrometer with a 6-stage cascaded MZI structure and demonstrate <10 pm resolution with >200 nm bandwidth using only 729 sampling channels. This achieves a bandwidth-to-resolution ratio of over 20,000, which is, to our best knowledge, about one order of magnitude greater than any reported miniaturized spectrometers to date.

N-glycosylation at N57/100/110 affects CD44s localization, function and stability in hepatocellular carcinoma.

The glycosylation levels of proteins in cancer cells are closely related to cancer invasion and migration. CD44 is a transmembrane glycoprotein that is significantly overexpressed in a variety of tumor cells and has been proven to promote the migration and motility of cancer cells, but the effect of its N-glycosylation modification on CD44 protein function in tumors is less studied. Here, we investigated the effect of six N-glycan chains (N25/57/100/110/120/255) on CD44s localization, function and stability in hepatocarcinoma cells. When the six sites were mutated, we found that CD44s lost its membrane localization in Huh7 and MHCC-97H cells. On this basis, we identified three glycosylation sites on CD44s (N57, N100 and N110) that played key roles in intracellular localization. When N57, N100 and N110 were mutated together, CD44 localized to the cytoplasm, while another three-site mutant (N25/N120/N255) was still anchored to the membrane. In addition, the ability of CD44-N57Q/N100Q/N110Q to promote the metastasis and invasion of Huh7 and 97H cells was weakened compared with that of CD44-N25Q/N120Q/N255Q. Furthermore, CD44-N57Q/N100Q/N110Q accumulated abnormally in the ER, and a high level of the ER stress (ERS) marker BiP was detected at the same time compared with wild-type CD44. When the lysosome inhibitor CQ was added, the content of mutant protein that triggered ERS significantly increased, which indicated that the degradation mode of CD44-N57Q/N100Q/N110Q after ERS was mainly through the lysosomal pathway (ERLAD). The results revealed that the N-glycosylation sites N57, N100 and N110 mutated on CD44s affected its function and degraded it by lysosomes after triggering ERS. These findings provide data for new studies on ER-related degradation, further promote the study of the glycan chain function of CD44 and furnish new ideas for the treatment of liver cancer metastasis.

N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the interaction of integrin ß1 with vimentin and promotes hepatocellular carcinoma cell motility.

N-glycosylation has been revealed to be tightly associated with cancer metastasis. As a key transferase that catalyzes the formation of ß1,4 N-acetylglucosamine (ß1,4GlcNAc) branches on the mannose core of N-glycans, N-acetylglucosaminyltransferase IVa (GnT-IVa) has been reported to be involved in hepatocellular carcinoma (HCC) metastasis by forming N-glycans; however, the underlying mechanisms are largely unknown. In the current study, we found that GnT-IVa was upregulated in HCC tissues and positively correlated with worse outcomes in HCC patients. We found that GnT-IVa could promote tumor growth in mice; notably, this effect was attenuated after mutating the enzymatic site (D445A) of GnT-IVa, suggesting that GnT-IVa regulated HCC progression by forming ß1,4GlcNAc branches. To mechanistically investigate the role of GnT-IVa in HCC, we conducted GSEA and GO functional analysis as well as in vitro experiments. The results showed that GnT-IVa could enhance HCC cell migration, invasion and adhesion ability and increase ß1,4GlcNAc branch glycans on integrin ß1 (ITGB1), a tumor-associated glycoprotein that is closely involved in cell motility by interacting with vimentin. Interruption of ß1,4GlcNAc branch glycan modification on ITGB1 could suppress the interaction of ITGB1 with vimentin and inhibit cell motility. These results revealed that GnT-IVa could promote HCC cell motility by affecting the biological functions of ITGB1 through N-glycosylation. In summary, our results revealed that GnT-IVa is highly expressed in HCC and can form ß1,4GlcNAc branches on ITGB1, which are essential for interactions with vimentin to promote HCC cell motility. These findings not only proposed a novel mechanism for GnT-IVa in HCC progression but also revealed the significance of N-glycosylation on ITGB1 during the process, which may provide a novel target for future HCC therapy.

Broadband picometer-scale resolution on-chip spectrometer with reconfigurable photonics.

Miniaturization of optical spectrometers is important to enable spectroscopic analysis to play a role in in situ, or even in vitro and in vivo characterization systems. However, scaled-down spectrometers generally exhibit a strong trade-off between spectral resolution and operating bandwidth, and are often engineered to identify signature spectral peaks only for specific applications. In this paper, we propose and demonstrate a novel global sampling strategy with distributed filters for generating ultra-broadband pseudo-random spectral responses. The geometry of all-pass ring filters is tailored to ensure small self- and cross-correlation for effective information acquisition across the whole spectrum, which dramatically reduces the requirement on sampling channels. We employ the power of reconfigurable photonics in spectrum shaping by embedding the engineered distributed filters. Using a moderate mesh of MZIs, we create 256 diverse spectral responses on a single chip and demonstrate a resolution of 20 pm for single spectral lines and 30 pm for dual spectral lines over a broad bandwidth of 115 nm, to the best of our knowledge achieving a new record of bandwidth-to-resolution ratio. Rigorous simulations reveal that this design will readily be able to achieve single-picometer-scale resolution. We further show that the reconfigurable photonics provides an extra degree of programmability, enabling user-defined features on resolution, computation complexity, and relative error. The use of SiN integration platform enables the spectrometer to exhibit excellent thermal stability of ±2.0 °C, effectively tackling the challenge of temperature variations at picometer-scale resolutions.

Advances in cost-effective integrated spectrometers.

The proliferation of Internet-of-Things has promoted a wide variety of emerging applications that require compact, lightweight, and low-cost optical spectrometers. While substantial progresses have been made in the miniaturization of spectrometers, most of them are with a major focus on the technical side but tend to feature a lower technology readiness level for manufacturability. More importantly, in spite of the advancement in miniaturized spectrometers, their performance and the metrics of real-life applications have seldomly been connected but are highly important. This review paper shows the market trend for chip-scale spectrometers and analyzes the key metrics that are required to adopt miniaturized spectrometers in real-life applications. Recent progress addressing the challenges of miniaturization of spectrometers is summarized, paying a special attention to the CMOS-compatible fabrication platform that shows a clear pathway to massive production. Insights for ways forward are also presented.

NEU4 inhibits motility of HCC cells by cleaving sialic acids on CD44.

Hepatocellular carcinoma (HCC) is an extremely metastatic tumor. Sialic acids (SAs) are associated with cancer development and metastasis. NEU4 is a sialidase that removes SAs from glycoconjugates, while the function of the NEU4 in HCC has not been clearly explored. In our research, we found the NEU4 expression was significantly down-regulated in HCC tissues, which was correlated with high grades and poor outcomes of HCC. The NEU4 expression could be regulated by histone acetylation. In the functional analysis of NEU4, the cell motility was inhibited when NEU4 was overexpressed, and restored when NEU4 expression was down-regulated. Similarly, NEU4 over-expressed HCC cells showed less metastasis in athymic nude mice. Further study revealed that NEU4 could inhibit cell migration by enzymatic decomposition of SAs. Our results verified a NEU4 active site (NEU4E235) and overexpressing inactivates NEU4E235A that weakens the inhibition ability to cell migration. Further, 70 kinds of specific interacting proteins of NEU4 including CD44 were identified through mass spectrum. Moreover, the α2,3-linked SAs on CD44 were decreased and the hyaluronic acid (HA) binding ability was increased when NEU4 over-expressed or activated. Additionally, the mutation of CD44 with six N-glycosylation sites showed less sensibility to NEU4 on cell migration compared with wild-type CD44. In summary, our results revealed the mechanism of low expression of NEU4 in HCC and its inhibitory effect on cell migration by removal of SAs on CD44, which may provide new treatment strategies to control the motility and metastasis of HCC.

Push-pull microring-assisted space-and-wavelength selective switch.

We introduce a novel design of a space-and-wavelength selective switch based on microring-assisted Mach-Zehnder interferometers. Multiple pairs of overcoupled microring resonators are incorporated as efficient and narrowband phase shifters and are driven in push-pull scheme. We design and demonstrate a 2×2×2λ elementary switch block with full spatial and spectral switching capabilities. The switching device's cross talk suppression and extinction ratio both exceed 21 dB. We measure over 75 GHz optical bandwidth per channel and less than 1.5 dB power penalty at 10-9 BER when two 32 Gbps on-off keying signals are loaded simultaneously. This new class of switching elements can further enable compact and high-performance space-and-wavelength selective switch fabrics without the need for wavelength (de)multiplexers or parallel switching planes.

Silicon photonic switch-based optical equalization for mitigating pulsewidth distortion.

Optical transmitters typically require electrical pre-amplification using driver amplifiers to optimize the optical modulation depth. To enhance the detection sensitivity and optimize the overall link budget, equalization is required to compensate for undesired signal distortion induced by the transmitter. In this paper, we propose and demonstrate a novel optical equalization scheme using a silicon photonic micro-ring resonator (MRR)-based switching circuit for mitigating driver-amplifier-induced pulsewidth distortion. The switching circuit simultaneously functions as a spatial optical switch as well as a two-stage optical bandpass filter for optical equalization. The experimental results indicate a 4.5-dB detection sensitivity enhancement at a data rate of 12.5 Gbits/s. The proposed approach is robust to different levels of pulsewidth distortion without additional signal processing, and has possibilities to support higher data rates by adjusting the MRR parameters.

Photonic switching in high performance datacenters [Invited].

Photonic switches are increasingly considered for insertion in high performance datacenter architectures to meet the growing performance demands of interconnection networks. We provide an overview of photonic switching technologies and develop an evaluation methodology for assessing their potential impact on datacenter performance. We begin with a review of three categories of optical switches, namely, free-space switches, III-V integrated switches and silicon integrated switches. The state-of-the-art of MEMS, LCOS, SOA, MZI and MRR switching technologies are covered, together with insights on their performance limitations and scalability considerations. The performance metrics that are required for optical switches to truly emerge in datacenters are discussed and summarized, with special focus on the switching time, cost, power consumption, scalability and optical power penalty. Furthermore, the Pareto front of the switch metric space is analyzed. Finally, we propose a hybrid integrated switch fabric design using the III-V/Si wafer bonding technique and investigate its potential impact on realizing reduced cost and power penalty.

Software-defined networking control plane for seamless integration of multiple silicon photonic switches in Datacom networks.

Silicon photonics based switches offer an effective option for the delivery of dynamic bandwidth for future large-scale Datacom systems while maintaining scalable energy efficiency. The integration of a silicon photonics-based optical switching fabric within electronic Datacom architectures requires novel network topologies and arbitration strategies to effectively manage the active elements in the network. We present a scalable software-defined networking control plane to integrate silicon photonic based switches with conventional Ethernet or InfiniBand networks. Our software-defined control plane manages both electronic packet switches and multiple silicon photonic switches for simultaneous packet and circuit switching. We built an experimental Dragonfly network testbed with 16 electronic packet switches and 2 silicon photonic switches to evaluate our control plane. Observed latencies occupied by each step of the switching procedure demonstrate a total of 344 µs control plane latency for data-center and high performance computing platforms.

Capacity limit for faster-than-Nyquist non-orthogonal frequency-division multiplexing signaling.

Faster-than-Nyquist (FTN) signal achieves higher spectral efficiency and capacity compared to Nyquist signal due to its smaller pulse interval or narrower subcarrier spacing. Shannon limit typically defines the upper-limit capacity of Nyquist signal. To the best of our knowledge, the mathematical expression for the capacity limit of FTN non-orthogonal frequency-division multiplexing (NOFDM) signal is first demonstrated in this paper. The mathematical expression shows that FTN NOFDM signal has the potential to achieve a higher capacity limit compared to Nyquist signal. In this paper, we demonstrate the principle of FTN NOFDM by taking fractional cosine transform-based NOFDM (FrCT-NOFDM) for instance. FrCT-NOFDM is first proposed and implemented by both simulation and experiment. When the bandwidth compression factor α is set to 0.8 in FrCT-NOFDM, the subcarrier spacing is equal to 40% of the symbol rate per subcarrier, thus the transmission rate is about 25% faster than Nyquist rate. FTN NOFDM with higher capacity would be promising in the future communication systems, especially in the bandwidth-limited applications.

Interleaved single-carrier frequency-division multiplexing for optical interconnects.

In this paper, we propose a real-valued interleaved single-carrier frequency-division multiplexing (I-SC-FDM) scheme for intensity-modulation and direct-detection optical interconnects. By simplifying the encoding structure, the computational complexity can be reduced from Nlog2N complex multiplications to N complex multiplications. At the complementary cumulative distribution function of 10-2, a reduction of 10 dB and 7.5 dB for the peak-to-average power ratio (PAPR) of the I-SC-FDM is achieved than that of orthogonal frequency-division multiplexing modulated with QPSK and 16QAM, respectively, when the subcarrier number is set to 4096. We experimentally demonstrate the I-SC-FDM scheme for optical interconnects with data rates of 12 Gbit/s, 24 Gbit/s and 128 Gbit/s transmitted over 22.5-km, 22.5-km and 2.4-km standard single mode fiber, respectively. The I-SC-FDM scheme shows great potential for cost-sensitive and power-sensitive optical interconnects owing to its low computational complexity and low PAPR.