RESUMO
BACKGROUND: Topography and tomography are valuable techniques for measuring the corneal shape, but they cannot directly assess its internal mechanical stresses. And nonuniform corneal stress plays a crucial biomechanical role in the progression of diseases and postoperative changes. Given the cornea's inherent transparency, analyzing corneal stresses using the photoelasticity method is highly advantageous. However, quantification of photoelasticity faces challenges in obtaining the stress-optic coefficient due to wrinkles caused by the non-spherical geometry during tensional experiments. OBJECTIVE: In this study, we propose an innovative experimental setup aimed at generating a gradient field of simple shear stress and achieving surface flatness during corneal stretching experiments, enabling the acquisition of the stress-optic coefficient through comparison with numerical results. METHODS: Our designed setup applies fluid pressure and force couples on the cornea. The internal fluid pressure maintains the corneal shape, preventing wrinkles, while the force couples create a stress field leading to isochromatic fringes. RESULTS: We successfully measured the stress-optic coefficients of the porcine anisotropic cornea in ex-vivo as 1.87 × 10-9 (horizontal) and 1.97 × 10-9 (vertical) (m2/N). Each isochromatic fringe order represents a shear stress range of 6.05 × 104 Pa under a low tension. CONCLUSIONS: This study establishes a significant connection between corneal photoelastic patterns and the quantification of corneal stress by enabling direct measurement through advanced photoelastic visualization technology for clinical applications.