Carbon dots derived from Poria cocos polysaccharide as an effective"on-off"fluorescence sensor for chromium(Ⅵ)detection / 药物分析学报

Chromium is a harmful contaminant showing mutagenicity and carcinogenicity.Therefore,detection of chromium requires the development of low-cost and high-sensitivity sensors.Herein,blue-fluorescent carbon quantum dots were synthesized by one-step hydrothermal method from alkali-soluble Poria cocos polysaccharide,which is green source,cheap and easy to obtain,and has no pharmacological ac-tivity due to low water solubility.These carbon quantum dots exhibit good fluorescence stability,water solubility,anti-interference and low cytotoxicity,and can be specifically combined with the detection of Cr(Ⅵ)to form a non-fluorescent complex that causes fluorescence quenching,so they can be used as a label-free nanosensor.High-sensitivity detection of Cr(Ⅵ)was achieved through internal filtering and static quenching effects.The fluorescence quenching degree of carbon dots fluorescent probe showed a good linear relationship with Cr(Ⅵ)concentration in the range of 1-100 μM.The linear equation was F0/F=0.9942+0.01472[Cr(Ⅵ)](R2=0.9922),and the detection limit can be as low as 0.25 μM(S/N=3),which has been successfully applied to Cr(Ⅵ)detection in actual water samples herein.

The Impact of Responsive Neurostimulation on the Treatment of Epilepsy.

There is a considerable number of patients with epilepsy that have drug resistant epilepsy (DRE). An additional option for these patients is resective surgery of ictal onset zones. However, a significant portion of DRE patients have unidentified or unresectable ictal zones. For these patients, RNS is a potential treatment option. The RNS system is a closed loop system that delivers stimulation in response to ECoG changes at seizure foci. It is programmed with an algorithm capable of detecting specific patterns of epileptogenic activity and triggers focal stimulation to interrupt seizures. The long term monitoring potential of the RNS system allows for a better understanding of the circadian rhythms behind epilepsy.

Comparison of treatment effect on hypertension ventricular hemorrhage using endoscope-controlled operations, Burr-hole craniotomy and urokinase perfusion through ventricular puncture / 中国综合临床

Objective To investigate the efficacy,safety and practicability of endoscope-controlled microneurosurgery operations for hypertension ventricular hemorrhage.Methods The efficacy and complications of three operation methods,including endoscope-controlled operation,Burr-hole craniotomy,ventricle puncture and drainage of urokinase infusion,were compared retrospectively.Results The complications of endoscopecontrolled operation was significantly lower than the other two methods(x2 =9.966,P ＜ 0.05).Among the 32patients treated by endoscope-controlled operation,2 patients died after the surgery with a fatality rate of 6.25％.Six months after the surgery,the ADL score estimation showed grade Ⅰ 2 cases,grade Ⅱ 14 cases; grade Ⅲ 11cases ; grade Ⅳ3 cases; grade V2 cases.Compared to the other two groups,there was significant difference(x2 =10.499,P ＜ 0.05).Conclusion Endoscope-controlled operation is an effective and safe method in treating patients with hypertension ventricular hemorrhage with less brain damage,better hemorrhage clearance,and less complications when compared with small bone window craniotomy and ventricle puncture and drainage of urokinase infusion operation methods.

Intraventricular epidermoids.

To study the clinical characteristics of intraventricular epidermoids, we analysed retrospectively 12 patients treated in our hospital and reviewed the literature with regard to clinical manifestations, imaging features, diagnosis, surgical procedures and prognosis. Four patients with lateral ventricle epidermoid and eight in the fourth ventricle were included in this group. Intraventricular epidermoids are characteristically hypodense non-enhancing lesions on CT scans. MRI reveals them to have long T1 and T2 relaxation times with slight mass effect. Total removal is ideal, but special attention should be paid to preservation of important neurovascular structures. Close proximity of tumours to cranial nerves and the brain stem pose technical difficulties in total removal. Cranial nerve dysfunction and aseptic meningitis are the main postoperative complications. Long-term prognosis for patients with intraventricular epidermoids and well-preserved neurological conditions is good, even in the case of subtotal excision. Clinical follow-up and MRI allow earlier diagnosis of recurrence.

Cerebellopontine angle epidermoids presenting with trigeminal neuralgia.

We studied the clinical characteristics of cerebellopontine angle (CPA) epidermoids presenting with trigeminal neuralgia (TN). Twenty-four patients were analyzed retrospectively and the literature reviewed with regard to clinical manifestation, imaging features, surgical procedures and prognosis. TN may be the initial symptom of CPA epidermoid, particularly in young patients. Epidermoids are characteristically hypodense nonenhancing lesions on CT scans, while on MRI they exhibit long T1 and T2 relaxation times. Although complete removal is ideal in the surgical management of CPA epidermoid, proximity to cranial nerves and the brain stem may pose technical difficulties in complete resection. In addition to complete resection of the tumour, arterial compression at the root entry zone (REZ) of the trigeminal nerve should be sought, and if found, a microvascular decompression (MVD) should be performed. Cranial nerve dysfunction and aseptic meningitis are the most common operative complications.

Cystic acoustic neuroma.

To define the clinical characteristics of cystic acoustic neuroma, we retrospectively analyzed 22 patients with cystic acoustic neuroma and reviewed the literature with regard to clinical manifestation, imaging features, diagnosis, surgical procedures and prognosis. An acoustic neuroma was defined as cystic according to the following criteria: the presence of hypodense/hypointense areas on CT or MRI, the identification of cystic elements at operation and histological verification. At the end of surgery, the facial nerve was anatomically intact in 86.4% of cystic acoustic neuromas. Complete removal of the tumor was achieved in 18 cases (81.8%). We conclude that patients with cystic acoustic neuroma need prompt surgery with special attention paid to the preservation of the facial nerve.

Neuroendoscopic anatomy and surgery of the cerebellopontine angle.

To probe the feasibility and utility of neuroendoscopic inspection of the anatomy of the cerebellopontine angle (CPA) and of neuroendoscopic assisted microneurosurgery (NEAMN) for CPA lesions via a retrosigmoid approach, we used retrosigmoid NEAMN in 28 patients with CPA lesions. Prior to this, we undertook anatomical observation of bilateral CPA in two adult cadaver heads using the neuroendoscope. NEAMN tumour resection was performed in eight acoustic neuromas, one meningioma and 14 cholesteatomas and NEAMN vascular decompression was performed in five patients with trigeminal neuralgia. Both the neurovascular structures of the CPA and the ventral surface of the pons, as well as the clivus, can be inspected using the neuroendoscope through a retrosigmoid approach with a 2-3 cm diameter bony opening. Complete excision of the tumour with preservation of the facial nerve was achieved in all eight acoustic neuromas. Likewise, total resection of the tumour was possible in the 14 cholesteatomas and one meningioma. Paroxysmal facial pain resolved after NEAMN vascular decompression in the five patients with trigeminal neuralgia. There were no postoperative complications or deaths in this series. The CPA can be divided into three levels - the cranial, medial, and caudal, and each level contains specific neurovascular structures as seen through the neuroendoscope. Knowledge of these divisions is useful to master the common NEAMN procedures of the CPA. NEAMN for CPA lesions via a retrosigmoid approach is a useful adjunct to standard microneurosurgical techniques effect and may decrease the operative risk.

Rerupture of intracranial aneurysms during cerebral angiography.

Three cases of re-rupture of intracranial aneurysms during cerebral angiography (RIADCA) between June and September, 2001 are reported. All cases underwent emergency craniotomy and aneurysm clipping. The subarachnoid blood and the extravasating contrast medium were removed intraoperatively as completely as possible. There was no mortality in this series. The incidence, timing, sex, age, inducing factors, risk factors, prevention measures and prognosis are discussed and reviewed in conjunction with the literature.

Chronic expanding intracerebral hematoma.

In order to study the clinical characteristics of chronic expanding intracerebral hematoma (CEICH), we analyzed retrospectively 21 patients with CEICH and reviewed the literature with regard to clinical manifestations, medical imaging features, surgical findings, pathological examinations, diagnoses, treatments and prognoses, etc. All patients recovered well and did not recur 1-2 months after operation, except one, who died of contralateral intracerebral hemorrhage three months later. Patients were followed-up by computerized tomographic (CT) scanning. MRI was useful for the preoperative diagnosis.

Intracranial tumoural haemorrhage--a report of 58 cases.

In order to study the computerized tomographic (CT) appearances and clinical characteristics of intracranial tumoural haemorrhage (ITH), we analyzed retrospectively fifty-eight patients with ITH and reviewed the literature. As a result, 91% patients had acute or subacute onset and 26% manifested haemorrhage as their first symptoms. CT scanning indicated that intratumoural bleeding occurred in 23 cases, bleeding into parenchyma 18 cases, subarachnoid space 6 cases, ventricle 3 cases and subdural space 8 cases. Thirty-eight patients had emergency operations and the others had selective operations. Both tumours and haematomas were removed all together in all patients. Fifty-five patients were cured or improved and three died during the perioperative stage in our series. Among the patients with ITH, there were 21 metastatic tumours, 19 gliomas, 10 meningiomas, 6 pituitary adenomas, 1 melanoma and 1 acoustic neurilemoma. The onset of most ITH resembled that of cerebrovascular diseases. The location of ITH and the CT appearances of ITH varied in different cerebral tumours. Radical removal of brain tumours with haemorrhage is an effective treatment for ITH, which can greatly decrease the perioperative mortality rate and improve the prognoses of patients.

Clinical characteristics of chronic subdural hematoma evolving from traumatic subdural effusion / 中华外科杂志

<p><b>OBJECTIVE</b>To probe into the incidence, mechanism and clinical characteristics of chronic subdural hematoma (CSDH) evolving from traumatic subdural effusion (TSE).</p><p><b>METHODS</b>The clinical materials of 32 patients with CSDH evolving from TSH were analyzed retrospectively and the correlative literature was reviewed.</p><p><b>RESULTS</b>16.7% of the patients with TSH evolved into CSDH. The time of evolution was 22 - 100 days after head injury. All patients were cured with hematoma drainage.</p><p><b>CONCLUSIONS</b>TSE is one of the origins of CSDH. The clinical characteristics of TSE evolving into CSDH include polarization of patient age, and chronic small effusion. The patients are usually injured deceleratedly and accompanied with mild cerebral damage.</p>

Protective effect of both human TH and GDNF cDNA-engineered SH-SY5Y cells on primary dopaminergic neurons / 中华老年医学杂志

Objective To construct genetically engineered cells that secrete human TH and GDNF at the same time by stable co-transfection pcDNA3.0/hTH and pcDNA3.1/hGDNF in SH-SY5Y cells and study their effects on the gene therapy of Parkinson′s disease (PD). Methods pcDNA3.0/hTH and pcDNA3.1/hGDNF were constructed by inserting human TH and GDNF cDNA into pcDNA3.0 and pcDNA3.1, respectively. SH-SY5Y cells were transfected with pcDNA3.0/hTH and pcDNA3.1/hGDNF and the positive cell clones of human TH and GDNF cDNA engineered cells could be identified by RT-PCR. The engineered cells were co-cultured with primary dopaminergic neurons in mouse. The number and growth condition of primary dopaminergic neurons were examined by Immunocytochemistry. Results The number of primary dopaminergic neurons protected by double-gene engineered cells increased at least by 2.8 times in comparison with the control cells(P