Absorption and metabolism of flavonoids in Herba Epimedii via rat intestinal perfusion model / 中国中药杂志

<p><b>OBJECTIVE</b>To study the absorption and metabolism of five flavonoids of Herba Epimedii (icariin, epimedin A, epi-medin B, epimedin C and baohuoside I) in rat intestine.</p><p><b>METHOD</b>Using the rat intestinal perfusion model, the contents of the five flavonoids in perfusates were measured by HPLC and the permeability coefficients in different intestines were calculated.</p><p><b>RESULT</b>The permeabilities of five flavonoids in duodenum, jejunum, ileum, colon according to the sequence were as: icariin: 6.365, 5.349, 1.004, 0.601, epimedinA: 4.120, 3.397, 0.401, 0.257, epimedin B: 3.645, 3.135, 0.990 7, 0.434, epimedin C: 3.114, 2.882, 0.688, 0.470, baohuoside I: 2.324, 2.398, 1.529, 1.045, respectively. The analysis of the perfusates showed that icariin, epimedin A, epimedin B, epimedin C could be hydrolyzed in intestine. Icariin had two hydrolytic metabolites, whereas epimedin A, B, C only had one hydrolytic metabolite, respectively.</p><p><b>CONCLUSION</b>The results indicated that the absorption of all the five flavonoids was significantly different in different intestinal segments, and the permeability in ileum and colon was significantly lower than that of the duodenum and jejunum. The metabolites of icariin, epimedin A, epimedin B, epimedin C were related with their structures and enzymes in intestine, and the metabolites were more in duodenum, jejunum, whereas were less in ileum and colon.</p>

Study on the Extraction and Purification Process of Schisandrin / 中国药房

OBJECTIVE: To optimize the process for extraction and purification of Schisandrin in Fructus Schisandra Chinensis. METHODS: The orthogonal test L9( 34) was adopted to optimize the water extraction process using Schisandrin and its extracts as indicators. The parallel test was used to optimize the parameters of the alcohol precipitation technique. RESULTS: The optimized process for extraction and purification of Schisandrin was as follows: extracting three times using 10- fold water, 1. 5h each time, with filter liquids concentrated to 1. 4g of crude drug mL- 1 then precipitated to 80% ; filtering alcohol liquids, and adjusting pH value to 7. CONCLUSIONS: This method can be used as a reference for the extraction of Fructus Schisandra Chinensis.

Extraction of Swertia chirayita(Roxb.ex Flemi) Karsten / 中成药

AIM:To determine the optimal extraction of effective component from Swertia chirayita(Roxb.ex Flemi) Karsten in Tibet. METHODS: HPLC method was used to determine the yield rate and mango glucoside content,the preference extract methods,extraction solvents and extraction method of Swertia chirayita(Roxb.ex Flemi) Karsten were chose by parallel comparative method. RESULTS: 75% ethanol was used to extract medicine materials by Percolate,the flow rate was 4 mL/min/kg,collecting 6 times liquor after percolate. CONCLUSION: The extraction method is simple and convenient,furthermore the extract stability is good too.

Study on Optimized Extraction of Fructus Schisandrae Chinensis / 中国药房

OBJECTIVE: To optimize the extraction process of Fructus Schisandrae Chinensis. METHODS: Taking the contents of schizandrin, schisantherin A, deoxyschizandrin, r-schizandrin as the indexes, the influence on the extraction process by extract solvent and medicinal material particle diameter was examined, and the extraction parameters were optimized. RESULTS: Active components of Fructus Schisandrae Chinensis, after the medicinal material being crushed, can be effectively utilized through extracting by alcohol. The optimized extraction parameters were as follows: the medical material being ground into 40 meshes, extracted twice by 70% alcohol, 2 hours per time. CONCLUSION: The optimized extracting process works better than current technique adopted for formulated granule preparation, and can ensure quality to the highest degree.