RESUMO
AIM: To investigate the cross-reactivity of the hyperimmune antisera of animals (rabbits) and the sera of oncological patients to polycyclic aromatic hydrocarbons (PAHs) of a similar chemical structure. METHODS: Reactions of antibodies with haptens have been estimated by non-competitive and competitive ELISA with synthesized protein conjugates of PAHs as a coating antigene. RESULTS: All the model rabbit antisera have been stated to react with anthracene, chrysene, pyrene, benzo(a)pyrene and benz(a)anthracene irrespective of the hapten used to immunize animals. The cross-reactivity of serum antibodies to all five PAHs has been found in blood samples of oncological patients. CONCLUSION: It is recommended to use the conjugates of anthracene, chrysene and pyrene for detecting human antibodies to more dangerous environmental carcinogens--benzo(a)pyrene and benz(a)anthracene.
Assuntos
Antígenos/metabolismo , Carcinógenos/análise , Haptenos/análise , Soros Imunes/análise , Neoplasias/sangue , Hidrocarbonetos Policíclicos Aromáticos/imunologia , Animais , Especificidade de Anticorpos , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática , Haptenos/química , Humanos , Imunização , Neoplasias/diagnóstico , Hidrocarbonetos Policíclicos Aromáticos/análise , Coelhos , Sensibilidade e Especificidade , Soroalbumina Bovina/químicaRESUMO
The method is described dealing with the synthesis of conjugates protein-polycyclic aromatic hydrocarbons (PAHs), highly soluble in water, stable without special stabilizers and containing the minimum quantity of cross-linked products. The reaction of protein with PAH containing an aldehyde group, has been carried out in an alkaline solution, and stabilization of the conjugate has been achieved by reduction with sodium borohydride in the presence of a compound blocking the formation of an insoluble polymeric fraction. The efficiency of synthesized conjugates for the induction and immunoassay of Abs to PAH for benzo[a]pyrene is shown.