[Lactate dehydrogenase isoenzyme content of the tissue of a cancerous tumor and polyps of the large intestine]. / O soderzhanii izoenzimov laktatdegidrogenazy v tkani rakovoi opukholi i polipov tolstoi kishki.

Electrophoretic assays of LDH-isoenzymes in agar were carried out in 599 homogenates of bioptic and resected tissue material obtained from carcinoma (174), polyps (305) and grossly normal mucosa (120). A significant considerable increase in cathode fractions of LDh4 and LDH5 matched by a decrease in LDH1, LDH2 and LDH3 levels was observed in both tumor and polyp tissue. The degree of the changes varied greatly depending on patients' age, type of tumor and size of polyp. Preoperative telegamma therapy was followed by a significant decrease in LDH5 and an increase in LDH2 levels.

[Changes in lactate dehydrogenase isoforms in the process of oncogenesis]. / Izmeneniia izoform laktatdegidrogenazy v protsesse onkogeneza.

Isoenzymes of lactate dehydrogenase were studied by disc-electrophoresis in polyacrylamide gel, and in the clinic--in 1% agar gel. Oncovirus A12 invasion of the culture of rat embryo fibroblasts (REF) was found to result in the increased percentage of the cathode fractions activity (LDG-4 and LD-5) and in the disappearance of LDG-1 yet during the first day of the experiment prior to hypoxia and enhanced proliferation, i. e. it is most likely to be primary. In the homogenates of cancerous tumor and large intestine polyps of man also a reliable increase of the cathode and a decrease or disappearance of the anode fractions accur. A correlation of the experimental and clinical data allowed a suggestion to be made that LDG isoenzymes changes are genetically conditioned and play an important role in the process of oncogenesis, providing conditions for the increased intensity of glycolysis and proliferation.

[Changes in blood serum proteins and blood electrolytes in patients with rectal fistulas and hemorrhoids]. / Izmeneniia belkov syvorotki i elektrolitov krovi u bol'nykh so svishchami priamoi kishki i gemorroem

Changes in blood serum and electrolytes were studied in 113 patients with rectal fistulas and hemorrhoid. The data obtained provided the opportunity for estimating objectively the intensity of inflammatory and destructive processes in pararectal cellular tissue.