[Functional and morphologic changes in the tendons and multimicrovacuolar collagen absorbing system of the three-phalanx fingers in disorder of arterial inflow].

Taking into account the fact, that unsatisfactory results of treatment of the thumbs traumatic damage are significantly caused by presence of anatomic disruption of the thumbs palm's own arteries with chronic tissue ischemia occurrence. One of consequences of damage of the three-phalanx thumbs interphalanx joints and the thumbs palm's own arteries is a formation of quickly progressing secondary flexing contracture of proximal interphalanx joints (PIPHJ). In the investigations accomplished there was established, that the changed tissues of the flexors tendons and their sheaths, forming cicatricial blockade distally to the primary trauma site, are taking part in a PIPHJ contracture development while arterial perfusion presence.The tissues damage severity, as well as a severity of clinical features of the tendons and their sheaths damage, depends on a degree of an arterial perfusion disorder present,

[Experiment study of multimicrovacuolar absorbing collagenic system and its role in trophic processes of nonsynovial part of the tendons].

The arterial inflow disorder and occurrence of an acute and subsequent chronic ischemia constitutes frequent complication of a hand and fingers trauma. One of the consequences of ischemic damage of tissues is formation of a flexor contracture of fingers, involving the flexors tendons. In extrasheath zone the blood supply is secured by paratendon--a connective tissue, taking part in the tendons sliding. According to data, obtained during morphological investigation of paratendon in laboratory animals, there was established its principal similarity with multimicrovacuolar collagenic absorbing system of a man. She constitutes a spatial porous structure, packed with cellular elements and vessels. While simulation in experimental conditions of the blood flow disorders, which in physiological environment is secured by paratendon, alike in disorder of the bone-tendon and muscle-tendon pathways of blood supply, there were revealed the coarse changes in the tendon structure and pronounced adhesion of tendons with loss of their sliding capacity, what had permitted to consider the paratendon blood circulation a dominating one.

[Application of Rheosorbilactum in the therapy of traumatic shock: comparative study].

The authors have studied influence of infusion of 800 ml RHEOSORBILACTUM, 500 ml VENOFUNDIN and 800 ml of 0,9% isotonic solution of sodium chloride with speed of 50-55 ml/mines on hemostasis, the maintenance of a liquid in the thorax, hemodynamic parameters in 60 patients with traumatic shock. It was revealed that the use of investigated volume of RHEOSORBILACTUM increases reliably (P<0,05) oxygen delivery for the account of the expressed haemodynamic effect which duration is less than during the use of VENOFUNDIN. It was revealed also that RHEOSORBILACTUM increases reliably (P<0,05) quantity of liquid in the thorax without an considerable difference with other investigated medications. There was no influence of RHEOSORBILACTUM on time of trombus formation tests--prothrombin time, the international normalized index, activated partial thromboplastin time.

[Investigations of fibrin clots formation under the effect of thrombin and ancystron]. / Doslidzhennia formuvannia fibrynovykh zgustkiv za diï trombinu ta antsystronu.

It was shown that heterogeneous surfaces of two phases influence on fibrin films structures. The clot formed between two phases have the surface layer which is different from fibrin clot surface structure. Transmission electron microscopy revealed to determine the thickness of the surface layer for desAA- and desAABB-fibrin. The surface layer thickness is 25,8 and 29 nm for desAA- and desAABB-fibrin respectively.

[Plasminogen activation by antiplasminogen monoclonal antibody IV-1C. Properties and mechanism of reaction]. / Aktyvatsiia plazminhenu antyplazminohenovym monoklonal;nym antytilom IV-1c. Vlastyvosti ta mekhanizm reaktsiï

In review the results of investigation of plasminogen(Pg) activation by antiplasminogen monoclonal antibody IV-1c have been presented. Antigenic determinant of IV-1c was localized in Val709-Gly718 site of Pg protease domain. IV-1c completely inhibited the Pg activation by streptokinase, but increased the rate of Pg activation by t-PA and urokinase. Catalytic properties of plasmin in complex with IV-1c were studied. It was found that IV-1c induced catalytic activity in Pg-IV-1c complex. It was shown that Pg and IV-1c interacts in complex by two-centre mechanism: IV-1c binds with Pg by paratope and by N-terminal lysine of gamma-chain and Pg binds to IV-1c by one of the lysine binding sites and by V709-G718 site of protease domain. The influence of pH, temperature, 1.5 mM Ca2+, Mg2+, Sr2+, Ba2+, Co2+, Ni2+ cations and 10 mM Cl-, F-, Ac-, SO4(2-), HPO4(2-) anions on lag and fast phases of Pg activation by VI-1c was investigated. It was revealed that Val709-Gly718 site was determining in Pg activation by IV-1c and streptokinase.

[The cellular mechanism for the formation of lamellar bodies in type-II pneumocytes in rat lungs]. / Kletochnyi mekhanizm formirovaniia lamelliarnykh tel v pnevmotsitakh tipa II v legkikh krys.

Pneumocyte type II cells from lungs of native rats and of rats that inspired a hypoxic mixture of gases were investigated by transmission electron microscopy. In cells of experimental rats, membrane structures were found that well compare with lamellar bodies. Experimental results and analysis of literature allowed to put forward a hypothesis about the cell mechanism of formation of lamellar bodies from the spiral twisted membranes of the endoplasmic reticulum.

[Tannic acid as a stabilizer of liposomal lipids in electron microscopic research]. / Tanninovaia kislota kak stabilizator liposomnykh lipidov pri élektronno-mikroskopicheskikh issledovaniiakh.

Using electron microscopy the action of tannic acid on the morphology of positively charged liposomes were studied in suspension and after adsorption of the surface membrane of human red blood cell in vitro. After treatment with tannic acid and glutaraldehyde followed by postosmication, an irreversible reconstruction of liposome lipid material with formation of many layers with tight packed lamellae was shown. Similar structures were found on the surface of red blood cells. Monolayer microvesicles 30 nm in diameter were seen after glutaraldehyde fixation postosmication, and final treatment with tannic acid. Similar microvesicles were seen adsorbed on the plasma membrane.

[Structural changes in the liposomes and the lamellar packing of liposomal lipids exposed to glycerin]. / Izmenenie struktury liposom i lamelliarnoi upakovki liposomal'nykh lipidov pri vozdeistvii glitserina.

Glycerol-induced structural changes in multilamellar liposomes (ML) were studied by light and thin-section electron microscopy. It is shown that high concentrations of glycerol induce non-bilayer rearrangements of liposomal lipids in ML. The data obtained are discussed regarding the possible mechanisms of cryoprotector effects on natural (biological) and artificial (liposomal) membranes.